[1]李雪梅,南武娟.缺氧诱导因子-1α、Notch1和Jagged1共转染对急性心肌梗死模型大鼠心功能的改善作用[J].陕西医学杂志,2022,51(11):1346-1350.[doi:DOI:10.3969/j.issn.1000-7377.2022.11.006]
　LI Xuemei,NAN Wujuan.Improvement of co-transfection of hypoxia inducible factor-1 α,Notch1 and Jagged1 on cardiac function in rats with acute myocardial infarction[J].,2022,51(11):1346-1350.[doi:DOI:10.3969/j.issn.1000-7377.2022.11.006]
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缺氧诱导因子-1α、Notch1和Jagged1共转染对急性心肌梗死模型大鼠心功能的改善作用
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年11期
页码:
1346-1350
栏目:
基础研究
出版日期:
2022-11-05

文章信息/Info

Title:
Improvement of co-transfection of hypoxia inducible factor-1 α,Notch1 and Jagged1 on cardiac function in rats with acute myocardial infarction
作者:
李雪梅1; 南武娟2
(1.榆林市第一医院心血管内科,陕西 榆林 719000; 2.咸阳市第一人民医院急诊医学科,陕西 咸阳 712000)
Author(s):
LI Xuemei; NAN Wujuan
(Department of Cardiovascular Medicine,the First Hospital of Yulin,Yulin 719000,China)
关键词:
急性心肌梗死;  缺氧诱导因子-1α;  Notch1;  Jagged1;  心功能;  改善作用
Keywords:
Acute myocardial infarction;  Hypoxia inducible factor-1α;  Notch1;  Jagged1;  Cardiac function;  Improvement
分类号:
R 542.22
DOI:
DOI:10.3969/j.issn.1000-7377.2022.11.006
文献标志码:
A
摘要:
目的:探讨缺氧诱导因子-1α(HIF-1α)、Notch1和Jagged1共转染对急性心肌梗死(AMI)模型大鼠心功能的改善作用。方法:将30只雄性Wistar大鼠分为模型组(通过结扎冠状动脉左前降支诱发AMI)、共转染组(在结扎冠状动脉左前降支过程中将HIF-1α、Notch1、Jagged1共转染慢病毒注射到大鼠心脏的胸前组织)和对照组(接受假手术处理),每组10只。采用HE染色观察大鼠心肌组织病理学变化。通过蛋白印迹分析心肌组织HIF-1α、Notch1、Jagged1蛋白表达水平。通过反转录定量PCR(RT-qPCR)检测心肌组织HIF-1α、Notch1、Jagged1 mRNA表达水平。通过超声心动图检查大鼠的心功能指标,包括射血分数(EF)、左心室缩短分数(FS)、左心室舒张末期内径(LVEDD)和左心室收缩末期内径(LVESD)。结果:对照组大鼠心肌细胞膜完整,无炎症细胞浸润; 模型组心肌细胞溶解或破坏、心肌结构紊乱、出现大量成纤维细胞或胶原纤维及少量炎性细胞浸润; 共转染组上述组织病理学异常较模型组减轻。模型组HIF-1α、Notch1、Jagged1蛋白和mRNA相对表达水平较对照组降低,共转染组HIF-1α、Notch1、Jagged1蛋白和mRNA相对表达水平较模型组升高(均P<0.05)。模型组EF和FS较对照组降低,共转染组EF和FS较模型组升高(均P<0.05)。模型组LVEDD和LVESD较对照组升高,共转染组LVEDD和LVESD较模型组降低(均P<0.05)。结论:AMI模型大鼠外周血HIF-1α、Notch1、Jagged1表达水平降低,心功能下降。HIF-1α、Notch1、Jagged1共转染可改善AMI模型大鼠的心功能。
Abstract:
Objective:To investigate the improvement of co-transfection of hypoxia inducible factor-1α(HIF-1α),Notch1 and Jagged1 on cardiac function in rats with acute myocardial infarction(AMI).Methods:A total of 30 male Wistar rats were divided into model group(AMI induced by ligation of the left anterior descending coronary artery),co-transfection group(HIF-1α,Notch1,Jagged1 cotransfected lentivirus injected into the chest tissue of rat heart during ligation of the left anterior descending coronary artery)and control group(treated with sham operation),with 10 rats in each group.Histopathological changes of rat myocardium were observed by HE staining.The expression levels of HIF-1α,Notch1 and Jagged1 proteins in myocardial tissue were analyzed by Western blot.The mRNA expression levels of HIF-1α,Notch1 and Jagged1 in myocardial tissue were detected by RT-qPCR.The cardiac function of rats was examined by echocardiography,including EF,FS,LVEDD and LVESD.Results:The myocardial cell membrane of the rats in the control group was intact without inflammatory cell infiltration; the myocardial cells in the model group were dissolved or destroyed,the myocardial structure was disordered,and a large number of fibroblasts or collagen fibers and a small amount of inflammatory cells infiltrated; the co-transfection group showed the abnormality of the above histopathology was less than that of the model group.The relative expression levels of HIF-1α,Notch1,Jagged1 protein and mRNA in the model group were lower than those in the control group,and the relative expression levels of HIF-1α,Notch1,Jagged1 protein and mRNA in the co-transfection group were higher than those in the model group(all P<0.05).The EF and FS in the model group were lower than those in the control group,and the EF and FS in the co-transfection group were higher than those in the model group(all P<0.05).The LVEDD and LVESD in the model group were higher than those in the control group,and the LVEDD and LVESD in the co-transfection group were lower than those in the model group(all P<0.05).Conclusion:The expression levels of HIF-1α,Notch1 and Jagged1 in peripheral blood of AMI rats are decreased,and the cardiac function is decreased.Co-transfection with HIF-1α,Notch1 and Jagged1 can improve cardiac function in rats with AMI.

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备注/Memo

备注/Memo:
基金项目:陕西省卫生健康科研基金资助项目(2021D011)
更新日期/Last Update: 2022-11-09