[1]张锦华,高 明,朱小静,等.促红细胞生成素对高糖诱导大鼠近端肾小管上皮细胞转分化的影响[J].陕西医学杂志,2022,51(10):1183-1186,1200.[doi:DOI:10.3969/j.issn.1000-7377.2022.10.002]
　ZHANG Jinhua,GAO Ming,ZHU Xiaojing,et al.Effect of EPO on transdifferentiation of rat proximal renal tubular epithelial cells induced by high glucose[J].,2022,51(10):1183-1186,1200.[doi:DOI:10.3969/j.issn.1000-7377.2022.10.002]
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促红细胞生成素对高糖诱导大鼠近端肾小管上皮细胞转分化的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年10期
页码:
1183-1186,1200
栏目:
基础研究
出版日期:
2022-10-05

文章信息/Info

Title:
Effect of EPO on transdifferentiation of rat proximal renal tubular epithelial cells induced by high glucose
作者:
张锦华; 高 明; 朱小静; 李立立
(西安市人民医院 西安市第四医院肾脏内科,陕西 西安710004)
Author(s):
ZHANG Jinhua; GAO Ming; ZHU Xiaojing; LI Lili
(Department of Nephrology,Xi'an People's Hospital,Xi'an 710004,China)
关键词:
糖尿病肾病;  促红细胞生成素;  促红素受体;  近端肾小管上皮细胞;  上皮间充质转分化;  氧化应激
Keywords:
Diabetic nephropathy;  Erythropoietin;  EPOR;  Proximal renal tubular epithelial cells;  Epithelial-mesenchymal transition;  Oxidative stress
分类号:
R 587.2
DOI:
DOI:10.3969/j.issn.1000-7377.2022.10.002
文献标志码:
A
摘要:
目的:研究促红细胞生成素(EPO)对高糖诱导的大鼠近端肾小管上皮细胞(NRK52E)转分化(EMT)的影响及其分子机制。方法:在25 mmol/L浓度葡萄糖培养基中培养NRK52E,建立高糖诱导的肾小管上皮细胞EMT模型。实验分为正常对照组(NC组)、甘露醇对照组(OC组)、高葡萄糖浓度组(HG组)、HG+EPO 50 U/ml组(E1组)以及HG+EPO 100 U/ml组(E2组)。其中NC组培养在正常环境中,OC组培养在19.5 mmol/L甘露醇环境中,其余三组培养在高糖环境中,且E1、E2组按要求添加相应浓度的EPO。RT-PCR检测促红素受体(EPOR)、E钙粘蛋白(E-cadherin)及α平滑肌肌动蛋白(α-SMA)mRNA的表达,Western blot检测EPOR、E-cadherin及α-SMA蛋白表达; 荧光探针检测胞内活性氧(ROS)水平。结果:高糖培养成功诱导NRK52E细胞EMT模型,相较于NC组,HG组细胞EPOR、α-SMA表达增加,E-cadherin表达降低,细胞ROS水平显著增加(均P<0.05); EPO干预抑制NRK52E细胞EMT,与HG组比较,E1和E2组E-cadherin表达增加,α-SMA表达降低,细胞内ROS水平降低,且E2组变化更显著。结论:EPO可能通过EPO受体的介导,缓解高糖诱导的氧化应激,抑制高糖诱导的大鼠近端肾小管上皮细胞表型转化。
Abstract:
Objective:To study the effect of erythropoietin(EPO)on the mesenchymal transition(EMT)of rat proximal renal tubular epithelial cells induced by high glucose and its molecular mechanism.Methods:NRK52E cells were cultured in 25 mmol/L glucose medium to establish the EMT model.The experiment was divided into normal control group(NC group),mannitol control group(OC group),high glucose concentration group(HG group),HG+EPO 50 U/ml group(E1 group)and HG+EPO 100 U/ml group(E2 group).The NC group was cultured in normal environment,and the OC group was cultured in 19.5 mmol/L mannitol environment,and the other three groups were cultured in high glucose environment.Moreover,E1 and E2 groups were supplemented with corresponding concentrations of EPO as required.The expression of EPO receptor(EPOR),E-cadherin and α-SMA mRNA were detected by RT-PCR.Western blot was used to detect the protein expressions of EPOR,E-cadherin and α-SMA.Intracellular reactive oxygen species(ROS)were detected by fluorescence probe.Results:The EMT model of NRK52E cells was successfully induced by high glucose culture.Compared with NC group,the expression of EPOR and α-SMA in HG group was increased,the expression of E-cadherin decreased,and the ROS level was significantly increased(all P<0.05).EPO inhibited EMT in NRK52E cells,compared with HG group,E-cadherin expression increased,α-SMA expression and intracellular ROS level were decreased in E1 and E2 groups,and the changes were more significant in E2 group(all P<0.05).Conclusion:EPO may relieve oxidative stress induced by high glucose and inhibit phenotypic transformation of rat proximal renal tubular epithelial cells induced by high glucose through EPO receptor mediation.

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备注/Memo

备注/Memo:
基金项目:陕西省社会发展科技攻关项目(2016SF-101)
更新日期/Last Update: 2022-10-07