[1]范 阳,刘展会,豆涛涛,等.冷诱导RNA结合蛋白在体外循环期间导致急性肾损伤机制实验研究[J].陕西医学杂志,2022,51(8):929-933.[doi:DOI:10.3969/j.issn.1000-7377.2022.08.006]
　FAN Yang,LIU Zhanhui,DOU Taotao,et al.Mechanisms of cold-induced RNA-binding protein leading to acute kidney injury during cardiopulmonary bypass[J].,2022,51(8):929-933.[doi:DOI:10.3969/j.issn.1000-7377.2022.08.006]
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冷诱导RNA结合蛋白在体外循环期间导致急性肾损伤机制实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年8期
页码:
929-933
栏目:
基础研究
出版日期:
2022-08-05

文章信息/Info

Title:
Mechanisms of cold-induced RNA-binding protein leading to acute kidney injury during cardiopulmonary bypass
作者:
范 阳1; 刘展会2; 豆涛涛2; 张西安2
(1.西安医学院,陕西 西安 710021; 2.西安市第九医院,陕西 西安 710054)
Author(s):
FAN Yang; LIU Zhanhui; DOU Taotao; ZHANG Xi'an
(Xi'an Medical University,Xi'an 710021,China)
关键词:
冷诱导RNA结合蛋白;  体外循环;  炎症;  急性肾损伤;  低温;  缺氧
Keywords:
Cold-inducible RNA-binding protein;  Cardiopulmonary bypass;  Inflammation;  Acute kidney injury;  Hypothermia;  Hypoxia
分类号:
R 692.5
DOI:
DOI:10.3969/j.issn.1000-7377.2022.08.006
文献标志码:
A
摘要:
目的:探讨在体外循环(CPB)期间细胞外冷诱导RNA 结合蛋白(CIRP)对急性肾损伤(AKI)的影响,并探索其作用机制。方法:H9c2细胞在常温常氧(NN)、低温缺氧(HH)、HH后复温复氧(HHNN)和HHNN中加入CIRP抑制剂(C23)四种条件下培养。NRK-52E细胞进一步与H9c2细胞的培养基共同培养。通过ELISA评估CIRP、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)水平的表达。TUNEL染色检测NRK-52E细胞凋亡情况。结果:低温缺氧可诱导H9c2细胞分泌CIRP,并通过TLR-4/MyD88通路促进IL-6和TNF-α的表达。高表达的CIRP和其他炎症因子可诱导NRK-52E细胞凋亡。而且C23可以阻断CIRP介导的促炎反应和对NRK-52E细胞的损伤作用。结论:低温缺氧条件下心肌细胞释放大量CIRP,并激活炎性反应,通过TLR-4/MyD88通路释放TNF-α和IL-6,诱导肾小管上皮细胞凋亡。
Abstract:
Objective:To investigate the effect of extracellular cold-inducible RNA-binding protein(CIRP)on acute kidney injury(AKI)during cardiopulmonary bypass(CPB)and explore its mechanism of action.Methods:H9c2 cells were cultured in four conditions:normal temperature and normoxia(NN),hypothermia and hypoxia(HH),rewarming and reoxygenation after HH(HHNN)and adding CIRP inhibitor in HHNN(C23).NRK-52E cells were further incubated with the medium of H9c2 cells.The expression of CIRP,interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)levels were assessed by ELISA.The apoptosis of NRK-52E cells was detected by TUNEL staining.Results:Hypothermia and hypoxia could induce H9c2 cells to secrete CIRP,and promote the expression of IL-6 and TNF-α through the TLR-4/MyD88 pathway.High expression of CIRP and other inflammatory factors can induce apoptosis of NRK-52E cells.Moreover,C23 could block CIRP-mediated pro-inflammatory and damage to NRK-52E cells.Conclusion:Cardiomyocytes release a large amount of CIRP and activate inflammatory reaction under hypothermia and hypoxia.TNF-α and IL-6 are released through TLR-4/MyD88 pathway to induce apoptosis of renal tubular epithelial cells.

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更新日期/Last Update: 2022-08-04