[1]李福平,王 茂,邱春丽,等.莱菔硫烷联合维生素D对乳腺癌细胞增殖及凋亡的影响[J].陕西医学杂志,2022,51(8):916-920.[doi:DOI:10.3969/j.issn.1000-7377.2022.08.003]
　LI Fuping,WANG Mao,QIU Chunli,et al.Effect of sulforaphane combined with vitamin D on proliferation and apoptosis of breast cancer cells[J].,2022,51(8):916-920.[doi:DOI:10.3969/j.issn.1000-7377.2022.08.003]
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莱菔硫烷联合维生素D对乳腺癌细胞增殖及凋亡的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年8期
页码:
916-920
栏目:
基础研究
出版日期:
2022-08-05

文章信息/Info

Title:
Effect of sulforaphane combined with vitamin D on proliferation and apoptosis of breast cancer cells
作者:
李福平1; 王 茂2; 邱春丽3; 马海龙2; 李 宏2; 代引海2
(1.陕西中医药大学,陕西 咸阳 712046; 2.陕西中医药大学第二附属医院,陕西 咸阳 712000; 3.咸阳市中心医院肿瘤内科,陕西 咸阳 712000)
Author(s):
LI Fuping; WANG Mao; QIU Chunli; MA Hailong; LI Hong; DAI Yinhai
(Shaanxi University of Chinese Medicine,Xianyang 712046,China)
关键词:
乳腺癌;  MCF-7细胞;  莱菔硫烷;  维生素D;  细胞增殖;  细胞凋亡
Keywords:
Breast cancer;  MCF-7 cell;  Sulforaphane;  Vitamin D;  Cell proliferation;  Apoptosis
分类号:
R 737.9
DOI:
DOI:10.3969/j.issn.1000-7377.2022.08.003
文献标志码:
A
摘要:
目的:探究莱菔硫烷(SFN)联合维生素D(VD)对人乳腺癌MCF-7细胞增殖及凋亡的影响,为预防和控制乳腺癌提供新的策略。方法:实验分为空白对照组、SFN组、VD组和SFN+VD组。人乳腺癌MCF-7细胞培养至对数生长期,倒置显微镜、细胞血球板计数、CCK-8法和流式细胞术分别观察、分析和检测对照组和处理组干预MCF-7细胞后的细胞形态、数量、细胞增殖活力和细胞凋亡情况。结果:倒置显微镜下可以观察到,MCF-7细胞数量随药物浓度增加而逐渐减少,细胞形态也逐渐皱缩。细胞血球板计数结果显示,随着SFN或VD浓度的上升,人乳腺癌MCF-7细胞的数目随之下降。CCK-8检测结果表明,SFN和VD均可抑制MCF-7细胞增殖能力,且随药物浓度的增加,这种抑制效果更加显著; SFN+VD与两类药物单独作用相比,其对MCF-7细胞增殖的抑制效果更加显著,当药物浓度为10 μmol/L时,SFN组、VD组和SFN+VD组中的MCF-7细胞增殖活力分别为对照组的47.54%、43.38%和32.29%,差异有统计学意义(均P<0.01)。流式细胞术检测结果显示,MCF-7细胞的凋亡比例随药物浓度的增加而显著上升,且SFN联合VD组最为显著。其中,对照组、10 μmol/L的SFN组、VD组和SFN+VD处理组中的MCF-7细胞凋亡率分别为9.99%、33.51%、57.90%和90.83%,差异有统计学意义(均P<0.01)。结论:SFN联合VD能够加强对人乳腺癌MCF-7细胞生长的抑制作用,并诱导乳腺癌细胞凋亡。
Abstract:
Objective:To explore the effect of sulforaphane(SFN)combined with vitamin D(VD)on the proliferation and apoptosis of human MCF-7 breast cancer cells,and to provide new strategies for the prevention and control of breast cancer. Methods:The experiment consisted of blank control group,SFN treatment group,VD treatment group,and SFN+VD treatment group.MCF-7 cells were cultured to a logarithmic growth phase.The morphology,number,and cell proliferation activity and apoptosis of MCF-7 cells were observed,analyzed and detected by inverted microscope,counting plate,CCK-8 method and flow cytometry after intervention in control group and treatment groups.Results:Under inverted microscope,it can be observed that the number of MCF-7 cells decreased gradually with the increase of drug concentration,and the cell morphology also gradually shrank.The result of counting plate showed that the number of MCF-7 cells decreased with the increase of SFN or VD concentration.The result of CCK-8 test showed that SFN and VD could inhibit the proliferation of MCF-7 cells,and the inhibitory effect was more obvious with the increase of drug concentration.The inhibitory effect of SFN combined with VD on the proliferation of MCF-7 cells was more significant than that of the two substances alone.When the drug concentration was 10 μmol/L,the proliferation activity of MCF-7 cells in SFN group,VD group and SFN+VD group were 47.54%,43.38%,and 32.29% of control group respectively,and the differences were statistically significant(all P<0.01).The result of flow cytometry showed that the percentage of apoptotic cells increased significantly with increasing drug concentration,especially in SFN+VD group. The apoptosis rates of MCF-7 cells in control group,10 μmol/L SFN group,VD group and SFN+VD group were 9.99%,33.51%,57.90% and 90.83% respectively,and the differences were statistically significant(all P<0.01).Conclusion:SFN combined with VD can enhance the inhibitory effect on the growth of MCF-7 cells and induce apoptosis of MCF-7 cells.

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备注/Memo

备注/Memo:
基金项目:陕西省重点研发计划项目(2022SF-563); 陕西省咸阳市科技计划项目(2016K02-94); 陕西中医药大学学科创新团队项目(2019-SY03)
更新日期/Last Update: 2022-08-04