[1]陈艳丽,宋 鹏,刘接威,等.慢性鼻窦炎伴鼻息肉患者鼻腔黏膜组织白细胞介素-17A表达特征及检测临床意义[J].陕西医学杂志,2022,51(5):612-616.[doi:DOI:10.3969/j.issn.1000-7377.2022.05.023]
　CHEN Yanli,SONG Peng,LIU Jiewei,et al.Expression characteristics and clinical significance of interleukin-17A in nasal mucosa of patients with chronic rhinosinusitis with nasal polypsis[J].,2022,51(5):612-616.[doi:DOI:10.3969/j.issn.1000-7377.2022.05.023]
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慢性鼻窦炎伴鼻息肉患者鼻腔黏膜组织白细胞介素-17A表达特征及检测临床意义
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年5期
页码:
612-616
栏目:
临床检验
出版日期:
2022-05-05

文章信息/Info

Title:
Expression characteristics and clinical significance of interleukin-17A in nasal mucosa of patients with chronic rhinosinusitis with nasal polypsis
作者:
陈艳丽; 宋 鹏; 刘接威; 徐杨斌; 陈丽文
(宁德师范学院附属宁德市医院耳鼻咽喉头颈外科,福建 宁德352000)
Author(s):
CHEN Yanli; SONG Peng; LIU Jiewei; XU Yangbin; CHEN Liwen
(Department of Otolaryngology,Ningde Municipal Hospital of Ningde Normal University,Ningde 352000,China)
关键词:
鼻窦炎;  鼻息肉;  白细胞介素-17A;  嗜酸性粒细胞;  炎性反应;  相关性
Keywords:
Sinusitis;  Nasal polyps;  IL-17A;  Eosinophils;  Inflammatory response;  Correlation
分类号:
R 765.43
DOI:
DOI:10.3969/j.issn.1000-7377.2022.05.023
文献标志码:
A
摘要:
目的:探讨慢性鼻窦伴炎鼻息肉(CRSwNP)患者鼻腔黏膜组织白细胞介素-17A(IL-17A)表达特征。方法:纳入行鼻内镜手术治疗的CRSwNP患者共84例,其中嗜酸粒细胞性慢性鼻窦炎伴鼻息肉(ECRSwNP)组28例、非嗜酸粒细胞性慢性鼻窦炎伴鼻息肉(nECRSwNP)组56例; 分析两组鼻黏膜组织中IL-17A、CD8+、CD4+、IL-6、转化生长因子-β(TGF-β)及IL-13表达情况,采用FCM确定IL-17A来源,评价IL-17A与IL-6、TGF-β及IL-23表达的相关性。结果:nECRSwNP组IL-17A水平、产IL-17A淋巴细胞比例、产IL-17A的CD8+、CD4+T细胞比例及Tc17、Th17细胞比例分别为(157.82±15.89)pg/ml、(10.56±2.80)%、(6.41±1.65)%、(26.96±4.80)%、(3.95±1.87)%、(1.70±0.67)%,均显著高于ECRSwNP组的(9.10±2.33)pg/ml、(3.19±0.65)%、(2.88±0.62)%、(12.49±2.75)%、(1.63±0.93)%、(0.44±0.15)%(均P<0.01); FCM检测结果显示ECRSwNP组和nECRSwNP组患者IL-17A均主要由Tc17细胞和Th17细胞合成。Spearman相关性分析结果显示,IL-17A与IL-6间呈正相关(P<0.01)。结论:nECRSwNP患者鼻黏膜IL-17A高表达与Tc17、Th17细胞表达分化增强有关,同时IL-17A与IL-6水平间呈正相关,提示该类人群病变组织特殊炎性环境可刺激Tc17/Th17细胞分化。
Abstract:
Objective:To investigate the expression characteristics and clinical significance of interleukin-17A(IL-17A)in nasal mucosa of patients with chronic rhinosinusitis with nasal polypsis(CRSwNP).Methods:A total of 84 CRSwNP patients undergoing endoscopic sinus surgery were included,including 28 patients with eosinophilic chronic rhinosinusitis with nasal polyps(ECRSwNP)and 56 patients with non-eosinophilic chronic rhinosinusitis with nasal polyps(nECRSwNP).The expression levels of IL-17A,CD8+,CD4+,IL-6,transforming growth factor-β(TGF-β)and IL-13 in nasal mucosa of the two groups were analyzed.The source of IL-17A were determined by FCM,and the correlation between IL-17A and IL-6,TGF-β and IL-23 were evaluated.Results:The level of IL-17A,the proportion of IL-17A producing lymphocytes,the proportion of CD8+ and CD4+ T cells producing IL-17A,and the proportion of TC17 and Th17 cells in nECRSwNP group were(157.82±15.89)pg/ml,(10.56±2.80)%,(6.41±1.65)%,(26.96±4.80)%,(3.95±1.87)% and(1.70±0.67)%,and all of them were significantly higher than those in ECRSwNP group [(9.10±2.33)pg/ml,(3.19±0.65)%,(2.88±0.62)%,(12.49±2.75)%,(1.63±0.93)%,(0.44±0.15)%,all P<0.01].FCM results showed that IL-17A was mainly synthesized by Tc17 and Th17 cells in both groups.Spearman correlation analysis showed a positive correlation between IL-17A and IL-6(P<0.01).Conclusion:The high expression of IL-17A in nasal mucosa of patients with nECRSwNP is related to the enhanced expression and differentiation of Tc17 and Th17 cells,and there is a positive correlation between IL-17A and IL-6 levels,suggesting that the special inflammatory environment of pathological tissues in this population can stimulate the differentiation of Tc17 and Th17 cells.

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备注/Memo

备注/Memo:
基金项目:福建省自然科学基金资助项目(2021J011167)
更新日期/Last Update: 2022-05-05