[1]南武娟,文申英.急性冠脉综合征大鼠外周血巨噬细胞中脂质稳态与动脉粥样硬化指数相关性研究[J].陕西医学杂志,2022,51(4):401-405.[doi:DOI:10.3969/j.issn.1000-7377.2022.04.004]
　NAN Wujuan,WEN Shenying.Correlation between lipid homeostasis in peripheral blood macrophages and atherosclerosis index of rats model with acute coronary syndrome[J].,2022,51(4):401-405.[doi:DOI:10.3969/j.issn.1000-7377.2022.04.004]
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急性冠脉综合征大鼠外周血巨噬细胞中脂质稳态与动脉粥样硬化指数相关性研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年4期
页码:
401-405
栏目:
基础研究
出版日期:
2022-04-05

文章信息/Info

Title:
Correlation between lipid homeostasis in peripheral blood macrophages and atherosclerosis index of rats model with acute coronary syndrome
作者:
南武娟1; 文申英2
(1.咸阳市第一人民医院,陕西 咸阳 712000; 2.商洛市中心医院,陕西 商洛 726000)
Author(s):
NAN Wujuan; WEN Shenying
(Department of Emergency Medicine,the First People's Hospital of Xianyang,Xianyang 712000,China)
关键词:
急性冠状动脉综合征;  巨噬细胞;  脂质稳态;  动脉粥样硬化指数;  影响因素
Keywords:
Acute coronary syndrome;  Macrophage;  Lipid homeostasis;  Atherosclerosis index;  Influencing factor
分类号:
R 36
DOI:
DOI:10.3969/j.issn.1000-7377.2022.04.004
文献标志码:
A
摘要:
目的:探究急性冠状动脉综合征(ACS)模型大鼠外周血巨噬细胞中脂质稳态与动脉粥样硬化指数(AI)的关系。方法:20只大鼠随机分为对照组和模型组,每组10只,对照组大鼠给予皮下注射等体积生理盐水,模型组大鼠皮下注射30 mg/kg盐酸异丙肾上腺素建立ACS模型。HE染色观察两组大鼠心肌和主动脉组织形态学变化; 计算心肌梗死面积比例和易损指数; 酶法测定大鼠外周血巨噬细胞中脂质含量并计算AI值。结果:与对照组相比,模型组大鼠心肌梗死面积比例、易损指数、外周血巨噬细胞中总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)的表达水平、non-HDL-C和AI值均较高(均P<0.05),而外周血巨噬细胞中高密度脂蛋白(HDL-C)的表达水平较低(P<0.05)。Pearson相关性分析显示,大鼠AI值与TC(r=0.627,P<0.001)、TG(r=0.438,P<0.001)和LDL-C(r=0.699,P<0.001)呈正相关,与HDL-C(r=-0.521,P<0.001)呈负相关。多元线性回归分析显示,TC(β=0.616,P<0.001)、TG(β=0.215,P<0.001)、LDL-C(β=0.757,P<0.001)和HDL-C(β=-0.255,P<0.001)是AI值的独立影响因素。结论:ACS模型大鼠外周血巨噬细胞中脂质稳态与AI密切相关,TC、TG、LDL-C和HDL-C是AI值的独立影响因素。
Abstract:
Objective:To investigate the correlation between lipid homeostasis in peripheral blood macrophages and atherosclerosis index(AI)of rats model with acute coronary syndrome(ACS).Methods:20 rats were randomly divided into control group and model group,with 10 rats in each group.In the model group,the ACS model was established by subcutaneous injection of 30 mg/kg isoproterenol hydrochloride in rats.The rats in the control group were given subcutaneous injection of an equal volume of saline.The myocardial and aortic morphology of each group were observed by HE staining.The proportion of myocardial infarction and vulnerability index were calculated.The contents of lipids in peripheral blood macrophages were detected by enzymic method and AI was calculated.Results:Compared with control group,the proportion of myocardial infarction,vulnerability index,expression levels of total cholesterol(TC),triglyceride(TG)and low-density lipoprotein cholesterol(LDL-C),non-HDL-C and AI in model group were higher,while the high-density lipoprotein cholesterol(HDL-C)was lower(all P<0.05).Pearson correlation analysis showed that the AI was positively correlated with expression levels of TC,TG and LDL-C(r=0.627,0.438,0.699,all P<0.001),and was negatively correlated with expression level of HDL-C(r=-0.521,P<0.001).Multiple linear regression analysis showed that AI was independently associated with the expression levels of TC,TG,LDL-C and HDL-C(β=0.616,0.215,0.757,-0.255,all P<0.001).Conclusion:The lipid homeostasis in peripheral blood macrophages of ACS model rats is closely related to AI,and TC,TG,LDL-C and HDL-C are independent influencing factors of AI.

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备注/Memo

备注/Memo:
基金项目:陕西省卫生健康科研基金资助项目(2018C006)
更新日期/Last Update: 2022-04-07