[1]白治苗,刘玉锋,赵 乐,等.核因子-κB p65和骨桥蛋白在子宫内膜癌组织与细胞中的表达及相关性研究[J].陕西医学杂志,2022,51(3):370-374.[doi:DOI:10.3969/j.issn.1000-7377.2022.03.025]
　BAI Zhimiao,LIU Yufeng,ZHAO Le,et al.Expression of nuclear factor kappa B p65 and osteopontin in endometrial carcinoma and their correlation[J].,2022,51(3):370-374.[doi:DOI:10.3969/j.issn.1000-7377.2022.03.025]
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核因子-κB p65和骨桥蛋白在子宫内膜癌组织与细胞中的表达及相关性研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年3期
页码:
370-374
栏目:
临床病理
出版日期:
2022-03-05

文章信息/Info

Title:
Expression of nuclear factor kappa B p65 and osteopontin in endometrial carcinoma and their correlation
作者:
白治苗; 刘玉锋; 赵 乐; 郭玉琳
(榆林市第二医院妇科,陕西 榆林 719000)
Author(s):
BAI Zhimiao; LIU Yufeng; ZHAO Le; GUO Yulin
(Department of Gynecology,Yulin No.2 Hospital,Yulin 719000,China)
关键词:
子宫内膜腺癌;  核因子-κB;  骨桥蛋白;  分子通路;  相关性
Keywords:
Endometrial Cancer;  Nuclear factor kappa B;  Osteopontin;  Molecular pathway;  Correlation
分类号:
R 737.33
DOI:
DOI:10.3969/j.issn.1000-7377.2022.03.025
文献标志码:
A
摘要:
目的:探讨核因子-κB(NF-κB )p65和骨桥蛋白(OPN)在子宫内膜癌组织及细胞中的表达,并分析两者的相关性。方法:收集子宫内膜腺癌患者35例(子宫内膜腺癌组)、子宫内膜非典型增生患者35例(非典型增生内膜组)和正常子宫内膜患者35例(正常子宫内膜组),采用免疫组织化学法检测三组子宫内膜组织中NF-κB p65、OPN蛋白的表达。采用反转录PCR(RT-PCR)及Western blot检测正常内膜细胞及子宫内膜癌HEC-1-B细胞中两者蛋白及mRNA的相对表达水平。采用Pearson法分析NF-κB p65与OPN表达的相关性。结果:三组患者组NF-κB p65与OPN蛋白平均积分光密度(IOD)值比较差异有统计学意义(P<0.05)。子宫内膜腺癌组和非典型增生内膜组NF-κB p65与OPN蛋白IOD值高于正常子宫内膜组,且子宫内膜腺癌组高于非典型增生内膜组(均P<0.05)。Western blot和RT-PCR结果显示,NF-κB p65和OPN蛋白及mRNA在子宫内膜癌HEC-1-B细胞中的相对表达水平明显高于正常内膜细胞(均P<0.05)。Pearson相关性分析显示,子宫内膜腺癌组和非典型增生内膜组中,NF-κB p65和OPN蛋白表达呈正相关(r=0.78、0.88,均P<0.001),但在正常子宫内膜组中两者蛋白表达无相关性(P>0.05)。结论:NF-κB p65和OPN在子宫内膜癌组织及细胞中表达较高且呈正相关,有可能是形成该病的分子通路,为该病靶向治疗提供了理论依据及重要靶点。
Abstract:
Objective:To investigate the expression of nuclear factor kappa B(NF-κB )p65 and osteopontin(OPN)in endometrial carcinoma tissues and cells,and analyze the correlation between them.Methods:35 patients with endometrial adenocarcinoma(endometrial adenocarcinoma group),35 patients with endometrial atypical hyperplasia(atypical hyperplasia group)and 35 patients with normal endometrium(normal endometrium group)were collected.The expressions of NF-κB p65 and OPN in the endometrium of the three groups were detected by immunohistochemical method.RT-PCR and Western blot were used to detect the relative expression levels of their proteins and mRNAs in normal endometrial cells and endometrial cancer HEC-1-B cells.The correlation between NF-κB p65 and OPN expression was analyzed by Pearson method.Results:There was significant difference in average integrated optical density(IOD)of NF-κB p65 and OPN among three groups(P<0.05).The IOD values of NF-κB p65 and OPN in endometrial adenocarcinoma group and atypical hyperplasia group were higher than those in normal endometrium group,and endometrial adenocarcinoma group was higher than atypical hyperplasia group(all P<0.05).Western blot and RT-PCR results showed that the relative expression levels of NF-κB p65 and OPN proteins and mRNAs in endometrial carcinoma HEC-1-B cells were significantly higher than those in normal endometrial cells(all P<0.05).Pearson correlation analysis showed that there was a positive correlation between the expression of NF-κB p65 and OPN proteins in endometrial adenocarcinoma group and atypical hyperplasia group(r=0.78,0.88,all P<0.001),but there was no correlation in normal endometrium group(P>0.05).Conclusion:The expression of NF-κB p65 and OPN in endometrial cancer tissues and cells is high and positively correlated,which may be the molecular pathway of the disease,and provides a theoretical basis and important target for the targeted treatment.

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备注/Memo

备注/Memo:
基金项目:陕西省榆林市科技计划项目(YF-2020-055)
更新日期/Last Update: 2022-03-04