[1]左 楠,王 远,王 爽.骨髓间充质干细胞移植联合多点微量注射曲安奈德对兔耳增生性瘢痕及创面巨噬细胞极化的影响[J].陕西医学杂志,2022,51(2):140-144.[doi:DOI:10.3969/j.issn.1000-7377.2022.02.003]
　ZUO Nan,WANG Yuan,WANG Shuang.Effects of bone marrow mesenchymal stem cell transplantation combined with multi-point microinjection of triamcinolone acetonide on hypertrophic scars of rabbit ears and polarization of macrophages in wounds[J].,2022,51(2):140-144.[doi:DOI:10.3969/j.issn.1000-7377.2022.02.003]
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骨髓间充质干细胞移植联合多点微量注射曲安奈德对兔耳增生性瘢痕及创面巨噬细胞极化的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年2期
页码:
140-144
栏目:
基础研究
出版日期:
2022-02-05

文章信息/Info

Title:
Effects of bone marrow mesenchymal stem cell transplantation combined with multi-point microinjection of triamcinolone acetonide on hypertrophic scars of rabbit ears and polarization of macrophages in wounds
作者:
左 楠1; 王 远2; 王 爽3
(1.西北大学附属医院 西安市第三医院,陕西 西安 710018; 2.西安市第五医院,陕西 西安 710008; 3.西安交通大学第二附属医院,陕西 西安 710004)
Author(s):
ZUO Nan; WANG Yuan; WANG Shuang
(The Affiliated Hospital of Northwest University,Xi'an 710018,China)
关键词:
骨髓间充质干细胞;  曲安奈德;  巨噬细胞;  增生性瘢痕;  创面;  炎症因子
Keywords:
Bone marrow mesenchymal stem cells;  Triamcinolone acetonide;  Macrophages;  Hypertrophic scars;  Wound surface;  Inflammatory factor
分类号:
R 758.19
DOI:
DOI:10.3969/j.issn.1000-7377.2022.02.003
文献标志码:
A
摘要:
目的:研究骨髓间充质干细胞(BMSCs)联合曲安奈德治疗对兔耳增生性瘢痕形成的影响,以及对创面巨噬细胞极化的影响。方法:60只新西兰兔随机均分为模型组、曲安奈德组和联合治疗组,所有兔均在耳部建立1 cm×1 cm创面。创面建立第21天,模型组不予任何治疗,曲安奈德组在兔耳创面瘢痕内多点注射曲安奈德,联合治疗组在曲安奈德组治疗的基础上在兔耳创面瘢痕内注射BMSCs进行治疗。记录三组兔耳创面愈合和完全上皮化时间,测量创面瘢痕面积和突出高度、瘢痕指数并进行比较。实时荧光定量PCR法检测创面瘢痕炎症因子[肿瘤坏死因子-α(TNF-α)、白细胞介素质1β(IL-1β)、白细胞介素6(IL-6)和单核细胞趋化蛋白(MCP-1)]和巨噬细胞表面标志物[自然杀伤细胞表达的表面受体(CD16)、诱导性一氧化氮合酶(iNOS)、巨噬细胞甘露糖受体(CD206)和精氨酸酶1(Arg1)]表达水平并比较。结果:三组兔耳创面愈合时间比较无统计学差异(均P>0.05),曲安奈德组兔耳创面完全上皮化时间、创面瘢痕面积和突出高度均显著低于模型组,而显著高于联合治疗组(均P<0.05)。曲安奈德组兔耳创面瘢痕炎症因子(TNF-α、IL-1β、IL-6和MCP-1)和M1巨噬细胞表面标志物(CD16和iNOS)表达水平均显著低于模型组,而显著高于联合治疗组(均P<0.05)。曲安奈德组兔耳创面瘢痕M2巨噬细胞表面标志物(CD206和Arg1)表达水平均显著高于模型组,而显著低于联合治疗组(均P<0.05)。结论:BMSCs联合曲安奈德对兔耳增生性瘢痕的形成具有抑制作用,其机制可能与促进M2巨噬细胞极化,降低瘢痕的炎症反应有关。
Abstract:
Objective:To study the effect of bone marrow mesenchymal stem cells(BMSCs)combined with triamcinolone acetonide treatment on the formation of hypertrophic scars in rabbit ears and the effect on the polarization of macrophages on the wound surface.Methods:Sixty New Zealand rabbits were randomly divided into model group,triamcinolone acetonide group and combined treatment group.All rabbits had 1 cm×1 cm wounds in their ears.On the 21st day of wound establishment,the model group received no treatment.The triamcinolone acetonide group was given multiple injections of triamcinolone acetonide into the scar of the rabbit ears,and the combined treatment group was injected with BMSCs into the scar of the rabbit ears on the basis of the treatment of the triamcinolone acetonide group.The wound healing and complete epithelialization time of the rabbit ears in the three groups were recorded,and the scar area,protrusion height and scar index of the wound were measured and compared.The expression levels of inflammatory factors(TNF-α,IL-1β,IL-6 and MCP-1)and macrophage surface markers(CD16,iNOS,CD206 and Arg1)in wound scars were detected by real-time fluorescent quantitative PCR method and compared.Results:There was no significant difference in wound healing time between the three groups(P>0.05).The complete epithelialization time,wound scar area and protrusion height in the triamcinolone acetonide group were significantly lower than those in the model group,but significantly higher than those in the combined treatment group(all P<0.05).The expression levels of inflammatory factors(TNF-α,IL-1β,IL-6 and MCP-1)and M1 macrophage surface markers(CD16 and iNOS)in the triamcinolone acetonide group were significantly lower than those in the model group,but significantly higher than those in the combination treatment group(all P<0.05).The expression levels of M2 macrophage surface markers(CD206 and Arg1)in the triamcinolone acetonide group were significantly higher than those in the model group,but significantly lower than those in the combined treatment group(all P<0.05).Conclusion:BMSCs combined with triamcinolone acetonide has an inhibitory effect on the formation of hypertrophic scars in rabbit ears,and its mechanism may be related to promoting the polarization of M2 macrophages and reducing the inflammatory response of scars.

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学基础研究计划项目(2019JQ-965)
更新日期/Last Update: 2022-02-09