[1]刘云璐,王承龙,王 强,等.戊巴比妥钠诱导心力衰竭心肌细胞损伤模型的构建及鉴定[J].陕西医学杂志,2022,51(1):3-6.[doi:DOI:10.3969/j.issn.1000-7377.2022.01.001]
　LIU Yunlu,WANG Chenglong,WANG Qiang,et al.Construction and identification of pentobarbital sodium-induced heart failure myocardial cell injury model[J].,2022,51(1):3-6.[doi:DOI:10.3969/j.issn.1000-7377.2022.01.001]
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戊巴比妥钠诱导心力衰竭心肌细胞损伤模型的构建及鉴定
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年1期
页码:
3-6
栏目:
基础研究
出版日期:
2022-01-05

文章信息/Info

Title:
Construction and identification of pentobarbital sodium-induced heart failure myocardial cell injury model
作者:
刘云璐1; 2; 王承龙1; 2; 王 强3; 杨 静4
(1.中国中医科学院西苑医院,北京 100089; 2.国家中医心血管临床医学研究中心,北京 100089; 3.中国中医科学院望京医院,北京 100102; 4.中国中医科学院医学实验中心,北京 100010)
Author(s):
LIU Yunlu; WANG Chenglong; WANG Qiang; YANG Jing
(Xiyuan Hospital,Chinese Academy of Traditional Chinese Medicine,Beijing 100089,China)
关键词:
戊巴比妥钠;  H9c2;  心肌细胞;  心力衰竭;  心肌损伤模型
Keywords:
Pentobarbital sodium;  H9c2;  Cardiomyocytes;  Heart failure;  Cardiomyocytes injury model
分类号:
R 36
DOI:
DOI:10.3969/j.issn.1000-7377.2022.01.001
文献标志码:
A
摘要:
目的:探讨戊巴比妥钠诱导心力衰竭相关的H9c2大鼠心肌细胞损伤模型的制作并明确相关机制。方法:不同浓度不同造模时间戊巴比妥钠对H9c2心肌细胞进行干预,利用透射电镜检测细胞超微结构、三磷酸腺苷(ATP)、活性氧(ROS)、细胞凋亡、自噬等细胞改变。结果:模型组(戊巴比妥钠干预组)线粒体可见肿胀边缘不清、内膜破裂、嵴断裂等改变,并伴随自噬小体增多。模型组ATP含量显著减少(P<0.01),ROS水平明显增加,细胞凋亡率显示出增长趋势(P>0.05)。结论:戊巴比妥钠诱导的H9c2心肌细胞损伤模型符合心衰心肌细胞病理改变,明确戊巴比妥钠对心肌细胞的影响,可为体外心衰模型的研究提供基础理论。
Abstract:
Objective:To explore the establlshment of pentobarbital sodium induced heart failure cardiomyocytes injury model of rat H9c2 cells and the related mechanism.Methods:Different concentrations and modeling time of pentobarbital sodium intervened H9c2 cardiomyocytes.The changes of cell ultrastructure,ATP,reactive oxygen species(ROS),apoptosis and autophagy were detected by transmission electron microscope.Results:In the model group(pentobarbital sodium intervention group),mitochondria showed swelling,unclear edge,intimal rupture,ridge rupture and other changes,accompanied by the increase of autophagy bodies.In the model group,the content of ATP decreased significantly(P<0.01),the level of ROS increased significantly,and the apoptosis rate showed an increasing trend(P>0.05).Conclusion:The H9c2 cardiomyocytes injury model induced by pentobarbital sodium is consistent with the pathological changes of cardiomyocytes in heart failure.Clarifying the effect of pentobarbital sodium on cardiomyocytes can provide a basic theory for the study of heart failure model in vitro.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81874410,81703865); 中国中医科学院优秀青年科技人才项目(ZZ13-YQ-034)
更新日期/Last Update: 2022-01-09