[1]王 瑗,冯建梅,卫白杨.右美托咪定对坐骨神经慢性压迫损伤模型大鼠神经病理性疼痛的缓解作用及机制研究[J].陕西医学杂志,2021,50(12):1492-1495.[doi:DOI:10.3969/j.issn.1000-7377.2021.12.006]
　WANG Yuan,FENG Jianmei,WEI Baiyang.Alleviating effect and mechanism of dexmedetomidine on chronic neuropathic pain in rat model with chronic compression injury of sciatic nerve[J].,2021,50(12):1492-1495.[doi:DOI:10.3969/j.issn.1000-7377.2021.12.006]
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右美托咪定对坐骨神经慢性压迫损伤模型大鼠神经病理性疼痛的缓解作用及机制研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年12期
页码:
1492-1495
栏目:
基础研究
出版日期:
2021-12-05

文章信息/Info

Title:
Alleviating effect and mechanism of dexmedetomidine on chronic neuropathic pain in rat model with chronic compression injury of sciatic nerve
作者:
王 瑗; 冯建梅; 卫白杨
(空军军医大学唐都医院麻醉科,陕西 西安 710038)
Author(s):
WANG Yuan; FENG Jianmei; WEI Baiyang
(Department of Anesthesiology,Tangdu Hospital of Air Force Medical University,Xi'an 710038,China)
关键词:
坐骨神经慢性压迫损伤;  右美托咪定;  神经病理性疼痛;  高迁移率组蛋白1;  Toll样受体4;  糖基化终产物受体
Keywords:
Chronic constriction injury of the sciatic nerve;  Dexmedetomidine;  Neuropathic pain;  High mobility histone 1;  Toll-like receptor 4;  Receptor for advanced glycation endproduct
分类号:
R 745.4
DOI:
DOI:10.3969/j.issn.1000-7377.2021.12.006
文献标志码:
A
摘要:
目的:探究右美托咪定对坐骨神经慢性压迫损伤(CCI)模型大鼠神经病理性疼痛的缓解作用及机制。方法:选择100只雄性SD大鼠,随机分为空白对照组、模型组、右美托咪定低剂量组、右美托咪定中剂量组和右美托咪定高剂量组。除空白对照组外,其他四组均构建坐骨神经CCI模型。比较各组大鼠海马区高迁移率组蛋白1(HMGB1)、肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)表达水平,以及HMGB1及其下游Toll样受体4(TLR4)和糖基化终产物受体(RAGE)mRNA相对表达水平。结果:与空白对照组比较,模型组大鼠海马区HMGB1蛋白相对表达量、TNF-α和IL-1β表达水平、HMGB1及其下游TLR4和RAGE mRNA相对表达水平表达较高(均P<0.05)。与模型组比较,右美托咪定各剂量组上述指标均降低(均P<0.05)。结论:右美托咪定可以减轻坐骨神经CCI模型大鼠神经病理性疼痛,其机制可能与抑制HMGB1从而抑制星形胶质细胞活性和TLR4/核因子-κB信号通路有关。
Abstract:
Objective:To explore the alleviating effect and mechanism of dexmedetomidine on chronic neuropathic pain in rat model with chronic compression injury(CCI)of sciatic nerve.Methods:A total of 100 male SD rats were selected and randomly divided into blank control group,model group,low-dose dexmedetomidine group,middle-dose dexmedetomidine group,and high-dose dexmedetomidine group.Except for the blank control group,the sciatic nerve CCI model was constructed in the other four groups.The relative expression levels of high mobility histone 1(HMGB1),tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in the hippocampus of each group of rats,as well as the relative expression levels of HMGB1 and its downstream Toll-like receptor 4(TLR4)and receptor for advanced glycation endproduct(RAGE)mRNA were compared between groups.Results:Compared with the blank control group,the relative expression levels of HMGB1 protein,TNF-α and IL-1β,and the relative expression levels of HMGB1 and its downstream TLR4 and RAGE mRNA in the hippocampus of the model group were higher(all P<0.05).Compared with the model group,the above indicators in each dose group of dexmedetomidine were decreased(all P<0.05).Conclusion:Dexmedetomidine can alleviate neuropathic pain in sciatic nerve CCI model rats,and its mechanism may be related to the inhibition of HMGB1,thereby inhibiting the activity of astrocytes and the TLR4/NF-κB signaling pathway.

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学基础研究计划项目(2020JQ-950)
更新日期/Last Update: 2021-12-07