[1]樊 宇,张轶博.角质细胞生长因子-2在金黄色葡萄球菌所致肺部感染中的作用及机制研究[J].陕西医学杂志,2021,50(12):1482-1486.[doi:DOI:10.3969/j.issn.1000-7377.2021.12.004]
　FAN Yu,ZHANG Yibo.Role and mechanism of KGF-2 in lung infection induced by Staphylococcus aureus[J].,2021,50(12):1482-1486.[doi:DOI:10.3969/j.issn.1000-7377.2021.12.004]
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角质细胞生长因子-2在金黄色葡萄球菌所致肺部感染中的作用及机制研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年12期
页码:
1482-1486
栏目:
基础研究
出版日期:
2021-12-05

文章信息/Info

Title:
Role and mechanism of KGF-2 in lung infection induced by Staphylococcus aureus
作者:
樊 宇; 张轶博
(锦州医科大学基础医学院病原生物教研室,辽宁 锦州 121017)
Author(s):
FAN Yu; ZHANG Yibo
(Department of Pathogenic Biology,School of Basic Medicine, Jinzhou Medical University,Jinzhou 121017,China)
关键词:
肺部感染;  金黄色葡萄球菌;  角质细胞生长因子-2;  肺损伤;  巨噬细胞;  炎症反应
Keywords:
Lung infection;  Staphylococcus aureus;  Keratinocyte growth factor-2;  Lung injury;  Macrophage;  Inflammatory response
分类号:
R 563.1
DOI:
DOI:10.3969/j.issn.1000-7377.2021.12.004
文献标志码:
A
摘要:
目的:探讨角质细胞生长因子-2(KGF-2)在金黄色葡萄球菌所致肺部感染中的作用及机制。方法:使用经鼻滴入金黄色葡萄球菌(2×108 CFU/ml)构建肺部感染小鼠模型,以经鼻滴入等体积磷酸缓冲液作为对照组。滴入金黄色葡萄菌群1 h后,将肺部感染小鼠分别经尾静脉注射KGF-2(2.5、5、10 mg/kg)。滴入金黄色葡萄球菌24 h后麻醉处死小鼠,HE染色观察肺组织病理情况; 检测小鼠肺湿/干质量比和肺泡灌洗液中性粒细胞和白细胞水平; 采用酶联免疫法检测肺泡灌洗液炎性因子水平[肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和IL-1β]、氧化应激因子水平[髓过氧化物酶(MPO)、丙二醛(MDA)和总抗氧化能力(T-AOC)]以及巨噬细胞炎性蛋白2(MIP-2)水平; 使用半体内法检测各组小鼠肺泡巨噬细胞的吞噬功能。结果:对照组小鼠肺泡排列完整且无组织损伤,细菌组小鼠肺泡有炎性浸润和水肿,KGF-2不同剂量组小鼠肺组织炎性浸润和水肿情况得到明显改善。细菌组肺湿/干质量比和肺泡灌洗液中性粒细胞、白细胞、TNF-α、IL-6、IL-1β、MPO、MDA、MIP-2水平高于对照组,而T-AOC水平、肺泡巨噬细胞吞噬百分比和吞噬指数低于对照组(均P<0.05)。KGF-2不同剂量组肺湿/干质量比和肺泡灌洗液中性粒细胞、白细胞、TNF-α、IL-6、IL-1β、MPO、MDA水平均低于细菌组,而T-AOC水平、肺泡巨噬细胞吞噬百分比和吞噬指数高于细菌组(均P<0.05)。结论:KGF-2可以改善金黄色葡萄球菌所致肺部感染损伤和肺炎症反应,其机制可能与影响肺泡巨噬细胞功能有关。
Abstract:
Objective:To explore the role and mechanism of keratinocyte growth factor-2(KGF-2)in lung infection induced by Staphylococcus aureus.Methods:The models of lung infection mice were constructed by transnasal instillation of Staphylococcus aureus(2×108 CFU/ml),while those given transnasal instillation of the same volume of phosphate buffer were enrolled as control group.At 1 hour after Staphylococcus aureus instillation,lung infection mice were injected with KGF-2(2.5,5,10 mg/kg)through the tail vein.At 24 hours after Staphylococcus aureus instillation,the mice were anesthetized and then executed.The pathology of lung tissues was observed by HE staining.The lung wet/dry weight ratio and the levels of neutrophils and leukocytes in bronchoalveolar lavage fluid(BALF)were detected.The levels of inflammatory factors [tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),IL-1β],oxidative stress factors [myeloperoxidase(MPO),malondialdehyde(MDA),total antioxidant capacity(T-AOC)] and macrophage inflammatory protein 2(MIP-2)in BALF were detected by enzyme linked immunosorbent assay.The phagocytic function of alveolar macrophages was detected by semi-in vivo method.Results:In control group,there was no lung injury such as inflammatory infiltration and edema in lung tissues.In bacterial group,there was lung injury such as alveolar inflammatory infiltration and edema in lung tissues.Different doses of KGF-2 could significantly improve lung injury.The lung wet/dry weight ratio,the levels of neutrophils,leukocytes,TNF-α,IL-6,IL-1β,MPO,MDA and MIP-2 in bacterial group were higher than those in control group,while T-AOC level,phagocytic percentage and phagocytic index of alveolar macrophages were lower than those in control group(all P<0.05).The lung wet/dry weight ratio,the levels of neutrophils,leukocytes,TNF-α,IL-6,IL-1β,MPO and MDA in different doses of KGF-2 group were lower than those in bacterial group,while T-AOC level,phagocytic percentage and phagocytic index of alveolar macrophages were higher than those in bacterial group(all P<0.05).Conclusion:KGF-2 can improve lung injury and inflammatory response induced by Staphylococcus aureus,which may be related to affecting the function of alveolar macrophages.

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备注/Memo

备注/Memo:
基金项目:内蒙古自治区人类遗传病研究重点实验室开放课题基金资助项目(YC202105)
更新日期/Last Update: 2021-12-07