[1]刘博强,张伟宁,忽海洋.miRNA-26a-5p靶向调控蛋白酪氨酸磷酸酶基因对冠心病模型大鼠主动脉内皮细胞炎症损伤的影响[J].陕西医学杂志,2021,50(11):1349-1353.[doi:DOI:10.3969/j.issn.1000-7377.2021.11.007]
　LIU Boqiang,ZHANG Weining,HU Haiyang.Effect of targeted regulation of protein tyrosine phosphatase gene by miRNA-26a-5p on inflammatory injury of aortic endothelial cells in rats with coronary heart disease[J].,2021,50(11):1349-1353.[doi:DOI:10.3969/j.issn.1000-7377.2021.11.007]
点击复制

miRNA-26a-5p靶向调控蛋白酪氨酸磷酸酶基因对冠心病模型大鼠主动脉内皮细胞炎症损伤的影响
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年11期
页码:
1349-1353
栏目:
基础研究
出版日期:
2021-11-05

文章信息/Info

Title:
Effect of targeted regulation of protein tyrosine phosphatase gene by miRNA-26a-5p on inflammatory injury of aortic endothelial cells in rats with coronary heart disease
作者:
刘博强; 张伟宁; 忽海洋
(铜川市人民医院,陕西 铜川 727000)
Author(s):
LIU Boqiang; ZHANG Weining; HU Haiyang
(Tongchuan People's Hospital,Tongchuan 727000,China)
关键词:
微小RNA;  miRNA-26a-5p;  PTEN基因;  冠心病;  大鼠;  主动脉内皮细胞
Keywords:
microRNA;  miRNA-26a-5p;  PTEN gene;  Coronary heart disease;  Rats;  Aortic endothelial cells
分类号:
R 541.4
DOI:
DOI:10.3969/j.issn.1000-7377.2021.11.007
文献标志码:
A
摘要:
目的:探究miRNA-26a-5p靶向调控蛋白酪氨酸磷酸酶基因(PTEN)对冠心病(CHD)模型大鼠主动脉内皮细胞炎症损伤的影响。方法:选取健康SD大鼠22只,随机分成正常组和模型组,正常组大鼠不用进行特殊处理,模型组大鼠按高脂饲料喂养法制备成冠心病模型,对比两组大鼠血钙和血脂水平、大鼠主动脉内皮组织的miRNA-26-5p、PTEN、caspase-1 mRNA相对表达量及PTEN、caspase-1蛋白相对表达量。取模型组大鼠剩余的部分主动脉内皮组织进行细胞培养,经细胞转染后,分成空白对照组、阴性组、miRNA-26a-5p mimic组、miRNA-26a-5p inhibitor组和miRNA-26a-5p inhibitor+siPTEN组,对比五组的miRNA-26a-5p、PTEN蛋白,miRNA-26-5p、PTEN、caspase-1 mRNA相对表达量和4个炎症因子(IL-10、IL-1β、IL-6和IL-18)水平。结果:①与正常组对比,模型组的总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDLC)、低密度脂蛋白胆固醇(LDLC)和Ca2+水平均高于正常组(均P<0.05)。②模型组主动脉内皮组织miRNA-26a-5p水平低于正常组,PTEN、caspase-1 mRNA表达水平以及PTEN、caspase-1蛋白表达水平均高于正常组(均P<0.05)。③与空白对照组对比,miRNA-26a-5p mimic组miRNA-26a-5p相对表达升高,PTEN、caspase-1 mRNA相对表达降低,PTEN、caspase-1蛋白表达升高(均P<0.05); miRNA-26a-5p inhibitor组的miRNA-26a-5p相对表达降低,PTEN、caspase-1 mRNA相对表达升高,PTEN、caspase-1蛋白表达降低(均P<0.05)。与miRNA-26a-5p mimic组相比,miRNA-26a-5p inhibitor+siPTEN组的miRNA-26a-5p相对表达降低,PTEN、caspase-1 mRNA相对表达均升高,PTEN、caspase-1蛋白表达均降低(均P<0.05)。④与空白对照组、阴性组相比,miRNA-26a-5p mimic组IL-1β、IL-6和IL-18水平降低,IL-10升高(均P<0.05),miRNA-26a-5p inhibitor组与之相反(P<0.05); 与miRNA-26a-5p mimic组相比,miRNA-26a-5p inhibitor组IL-1β、IL-6和IL-18水平升高,IL-10降低(均P<0.05)。结论:冠心病大鼠主动脉内皮细胞中miRNA-26a-5p呈高表达、PTEN低表达,miRNA-26a-5p靶向调控PTEN能够使IL-1β、IL-6和IL-18炎症因子释放减少,减缓主动脉内皮细胞炎症损伤,具有内皮细胞保护作用。
Abstract:
Objective:To investigate the effect of targeted regulation of protein tyrosine phosphatase gene(PTEN)by miRNA-26a-5p on inflammatory injury of aortic endothelial cells in rats with coronary heart disease(CHD).Methods:22 healthy SD rats were randomly divided into normal group and model group.The rats in the normal group did not need special treatment.The rats in the model group were fed with high-fat diet to prepare the coronary heart disease model.The blood calcium and blood lipid levels,the expression levels of miRNA-26-5p,PTEN,caspase-1 mRNAs,and the expression levels of PTEN and caspase-1 proteins in aortic endothelium of the two groups were compared.Part of the aortic endothelial tissue of the model group was cut for cell culture.After cell transfection,the cells were divided into blank control group,negative control group,miRNA-26a-5p mimic group,miRNA-26a-5p inhibitor group and miRNA-26a-5p inhibitor + siPTEN group.The miRNA-26a-5p,PTEN mRNA,PTEN protein,caspase-1 mRNA,caspase-1 protein and four inflammatory factors(IL-10,IL-1,IL-6 and IL-18)in the five groups were compared.Results:①Compared with the normal group,the levels of TC,TG,LDLC and Ca2+ in the model group were significantly higher than those in the normal group(all P<0.05).②The level of miRNA-26a-5p in the model group was significantly lower than that in the normal group,and the expression levels of PTEN mRNA,caspase-1 mRNA,PTEN protein and caspase-1 protein were significantly higher than those in the normal group(all P<0.05).③Compared with the blank control group,the relative expression of miRNA-26a-5p increased significantly,the relative expression of PTEN and caspase-1 mRNAs decreased significantly,and the expression of PTEN and caspase-1 proteins increased significantly(all P<0.05).In the miRNA-26a-5p inhibitor group,the relative expression of miRNA-26a-5p decreased significantly,the relative expression of PTEN and caspase-1 mRNAs increased,and the expression of PTEN and caspase-1 proteins decreased(all P<0.05).Compared with miRNA-26a-5p mimic group,the relative expression of miRNA-26a-5p in miRNA-26a-5p inhibitor + siPTEN group decreased,the relative expression of PTEN and caspase-1 mRNAs increased,and the expression of PTEN and caspase-1 proteins decreased(all P<0.05).④Compared with blank control group and negative control group,the levels of IL-1β,IL-6 and IL-18 in miRNA-26a-5p mimic group decreased significantly,and IL-10 increased significantly,which was opposite in miRNA-26a-5p inhibitor group(all P<0.05); Compared with miRNA-26a-5p mimic group,miRNA-26a-5p inhibitor group,the levels of IL-1β,IL-6 and IL-18 increased significantly,and IL-10 decreased significantly(all P<0.05).Conclusion:MiRNA-26a-5p was highly expressed and PTEN was lowly expressed in aortic endothelial cells of rats with coronary heart disease.MiRNA-26a-5p targeting PTEN can increase IL-1β,IL-6 and IL-18,reduce the release of inflammatory factors,slow down the inflammatory injury of aortic endothelial cells,and have the protective effect of endothelial cells.

参考文献/References:

[1] 黄敏华,孙雁智.稳定性冠心病患者血清抵抗素水平与心肌梗死相关性研究[J].陕西医学杂志,2020,49(2):187-189,208.
[2] 杨自华,郭平学,王西辉,等.miRNA-244与miRNA-328在胆管癌患者血清中的表达及意义[J].陕西医学杂志,2020,49(5):549-552,556.
[3] 袁晓龙,潘金春,蒋 瑶,等.miR-144、miR-21-3p、miR-142-5p及miR-27b-3p对心肌梗死后血管再生的作用[J].中国实验动物学报,2020,28(3):297-306.
[4] 陈笑梅,牟雅琳,吕 鹏,等.miRNAs调控动脉粥样硬化相关细胞功能研究进展[J].厦门大学学报:自然科学版,2018,57(5):593-601.
[5] Jing R,Zhong QQ,Long TY,et al.Downregulated miRNA-26a-5p induces the apoptosis of endothelial cells in coronary heart disease by inhibiting PI3K/AKT pathway[J].Eur Rev Med Pharmacol Sci,2019,23(11):4940-4947.
[6] 王秋宇,朱军义,邓巧子.MiRNA-92a靶向PTEN通过PI3K/AKT信号通路促进卵巢癌细胞转移[J].临床与病理杂志,2020,40(7):1658-1667.
[7] 蒋长荣,马小峰.SATB1基因沉默介导NF-κB通路对冠心病小鼠主动脉内皮细胞炎症损伤的保护作用[J].东南大学学报:医学版,2020,39(1):35-40.
[8] 胡宇才,董 倩,秦元梅,等.冠心丹参滴丸治疗冠心病心绞痛疗效与安全性系统评价[J].陕西中医,2021,42(1):124-130.
[9] 朱小山,马可忠,周汉云,等.血同型半胱氨酸、高迁移率族蛋白B1与冠心病发生及严重程度的相关性研究[J].中西医结合心脑血管病杂志,2020,18(6):68-71.
[10] 艾 虎,郑耐心,李 乐,等.老年冠心病患者冠状动脉造影血流储备分数与导丝血流储备分数诊断价值的评价[J].中华老年医学杂志,2021,40(4):459-463.
[11] 张红斌,郭志浩,刘 飞,等.冠状动脉慢血流现象与左心功能和冠状动脉直径的相关性分析[J].解放军医药杂志,2020,32(4):52-55.
[12] 宋长来,付 新,孙江涛.冠脉内注射尼可地尔对NSTEMI患者经皮冠状动脉介入治疗术中慢血流/无复流的影响及危险因素分析[J].河北医学,2019,25(8):1249-1253.
[13] Qvd A,Ah B,Kz B,et al.Calcium overload decreases net free radical emission in cardiac mitochondria-science direct[J].Mitochondrion,2020,51(3):126-139.
[14] Song CL,Liu B,Diao HY,et al.Down-regulation of microRNA-320 suppresses cardiomyocyte apoptosis and protects against myocardial ischemia and reperfusion injury by targeting IGF-1[J].Oncotarget,2016,7(26):39740-39757.
[15] Caccioppo A,Franchin L,Grosso A,et al.Ischemia reperfusion injury: Mechanisms of damage/protection and novel strategies for cardiac recovery/regeneration[J].International Journal of Molecular Sciences,2019,20(20):5024.
[16] 崔 琴,屈振杰,陈 吉.miRNA-381靶向PTEN对胃癌细胞增殖与凋亡的影响及相关机制研究[J].临床和实验医学杂志,2020,19(18):1930-1933.
[17] 崔丹瑜,朱丁季,任 昊,等.循环型microRNA-26a及其靶基因PTEN与系统性红斑狼疮的相关性研究[J].中国现代医学杂志,2017,27(26):45-51.
[18] Wu K,Mu X,Jiang J,et al.miRNA-26a-5p and miRNA2659 inhibit the proliferation of bladder cander cells by regulating PDCD10[J].Oncol Rep,2018,40(6): 3523-3532.
[19] 安依涵,杜 璟,侯志文,等.双氢青蒿素抑制PTEN/AKT通路诱导急性髓系白血病细胞凋亡[J].中国实验血液学杂志,2020,28(1): 88-92.
[20] 刘梦涵,杨 琳,刘小军,等.靶向抑制miRNA-21对K562细胞增殖及PTEN-PI3K/AKT通路的影响[J].中华血液学杂志,2016,37(11):982-986.

相似文献/References:

[1]赵 敏,赵 琳,钟社兰.MiR-123调控Bcl-2对宫颈癌细胞侵袭及转移能力的影响*[J].陕西医学杂志,2020,49(5):542.
　ZHAO Min,ZHAO Lin,ZHONG Shelan..Effect of miR-123 regulating Bcl-2 on the invasion and metastasis of cervical cancer[J].,2020,49(11):542.
[2]韦佩祺,苏梓健,梁 芳,等.microRNA在系统性红斑狼疮中的作用机制研究进展[J].陕西医学杂志,2023,52(2):238.[doi:DOI:10.3969/j.issn.1000-7377.2023.02.027]
　WEI Peiqi,SU Zijian,LIANG Fang,et al.Research progress on the mechanism of microRNA in systemic lupus erythematosus and the intervention of traditional Chinese medicine[J].,2023,52(11):238.[doi:DOI:10.3969/j.issn.1000-7377.2023.02.027]

备注/Memo

备注/Memo:
基金项目:陕西省卫生计生科研基金资助项目(2016D077)
更新日期/Last Update: 2021-11-05