[1]谢江涛,冯乐宵,王世锋,等.常氧及低氧条件下SHG-44胶质瘤细胞中活性氧及低氧诱导因子-1α的表达及意义[J].陕西医学杂志,2021,50(11):1345-1348.[doi:DOI:10.3969/j.issn.1000-7377.2021.11.006]
 XIE Jiangtao,FENG Lexiao,WANG Shifeng,et al.Expression and significance of reactive oxygen species and hypoxia-inducible factor-1α in SHG-44 glioma cells under normoxia and hypoxia[J].,2021,50(11):1345-1348.[doi:DOI:10.3969/j.issn.1000-7377.2021.11.006]
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常氧及低氧条件下SHG-44胶质瘤细胞中活性氧及低氧诱导因子-1α的表达及意义
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年11期
页码:
1345-1348
栏目:
基础研究
出版日期:
2021-11-05

文章信息/Info

Title:
Expression and significance of reactive oxygen species and hypoxia-inducible factor-1α in SHG-44 glioma cells under normoxia and hypoxia
作者:
谢江涛1冯乐宵1王世锋1刘振锋1李云翔2
(1.咸阳市中心医院神经外科,陕西 咸阳712000; 2.乾县人民医院神经外科,陕西 乾县713300)
Author(s):
XIE JiangtaoFENG LexiaoWANG ShifengLIU ZhenfengLI Yunxiang
(Department of Neurosurgery,Xianyang Central Hospital,Xianyang 712000,China)
关键词:
胶质瘤 活性氧 低氧诱导因子-1α N-乙酰半胱氨酸 氯化钴
Keywords:
Glioma Reactive oxygen species Hypoxia inducible factor-1α N-acetylcysteine Cobalt chloride
分类号:
R 739.41
DOI:
DOI:10.3969/j.issn.1000-7377.2021.11.006
文献标志码:
A
摘要:
目的:研究常氧及低氧条件下SHG-44胶质瘤细胞中活性氧(ROS)及低氧诱导因子-1α(HIF-1α)的表达及意义。方法:在常氧条件及低氧条件下培养SHG-44胶质瘤细胞,分为常氧对照组、常氧NAC干预组、低氧对照组、低NAC氧干预组四组。常氧及低氧干预组加入N-乙酰半胱氨酸(NAC),48 h后检测ROS的表达,RT-PCR检测HIF-1α mRNA表达。结果:低氧对照组ROS表达水平明显高于常氧对照组(P<0.05),常氧NAC干预组及低氧NAC干预组给予抗氧化剂NAC之后ROS表达水平均明显下降(均P<0.05); 低氧对照组HIF-1α mRNA表达高于常氧对照组、常氧NAC干预组及低氧NAC干预组(均P<0.05)。结论:低氧条件下ROS及HIF-1α mRNA表达水平明显高于常氧条件,但是应用ROS干预剂NAC之后低氧条件下SHG-44胶质瘤细胞中HIF-1α mRNA表达下降,提示ROS在促进HIF-1α mRNA表达中起重要作用。
Abstract:
Objective:To study the expression and significance of reactive oxygen species(ROS)and hypoxia inducible factor-1α(HIF-1α)in SHG-44 glioma cells under normoxia and hypoxia.Methods:SHG-44 glioma cells were cultured under normoxia and hypoxia conditions and divided into normoxia control group,normoxia intervention group,hypoxia control group,and hypoxia intervention group.The normoxia and hypoxia intervention groups were added with N-acetylcysteine(NAC),and the expression of ROS was detected 48 hours later,and the expression of HIF-1α mRNA was detected by RT-PCR.Results:The expression level of ROS in the hypoxic control group was significantly higher than that of the normoxic control group(all P<0.05).The expression of ROS in the normoxia intervention group and the hypoxia intervention group was significantly reduced after the antioxidant NAC was given(all P<0.05).The expression of HIF-1α mRNA in the hypoxia control group was higher than that of the normoxia control group,the normoxia intervention group and the hypoxia intervention group(all P<0.05).Conclusion:The expression levels of ROS and HIF-1α mRNA under hypoxic conditions were significantly higher than those under normoxia conditions.However,the expression of HIF-1α mRNA in SHG-44 glioma cells decreased under hypoxic conditions after the application of NAC,an intervention agent of ROS.ROS play an important role in promoting the expression of HIF-1α mRNA.

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更新日期/Last Update: 2021-11-05