[1]洪 甲,杨 洋,栾丽霞.lncRNA-p23154通过miR-375/KLF4途径对SKOV3细胞增殖、侵袭的影响[J].陕西医学杂志,2021,50(11):1336-1339.[doi:DOI:10.3969/j.issn.1000-7377.2021.11.004]
　HONG Jia,YANG Yang,LUAN Lixia.Effect of lncRNA-p23154 on proliferation and invasion of SKOV3 cells through miR-375/Krüppel-like factor 4 pathway[J].,2021,50(11):1336-1339.[doi:DOI:10.3969/j.issn.1000-7377.2021.11.004]
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lncRNA-p23154通过miR-375/KLF4途径对SKOV3细胞增殖、侵袭的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年11期
页码:
1336-1339
栏目:
基础研究
出版日期:
2021-11-05

文章信息/Info

Title:
Effect of lncRNA-p23154 on proliferation and invasion of SKOV3 cells through miR-375/Krüppel-like factor 4 pathway
作者:
洪 甲1; 杨 洋2; 栾丽霞2
(1.西安国际医学中心医院产科,陕西 西安 710100; 2.西安医学院第一附医院妇科,陕西 西安 710077)
Author(s):
HONG Jia; YANG Yang; LUAN Lixia
(Department of Obstetrics,Xi'an International Medical Center Hospital,Xi'an 710100,China)
关键词:
长链非编码RNA;  微小RNA;  卵巢上皮性癌;  SKOV3细胞;  细胞增殖;  细胞侵袭
Keywords:
Long non-coding RNA;  microRNA;  Epithelial ovarian cancer;  SKOV3 cells;  Cell proliferation;  Cell invasion
分类号:
R 737.31
DOI:
DOI:10.3969/j.issn.1000-7377.2021.11.004
文献标志码:
A
摘要:
目的:研究长链非编码RNA-p23154(lncRNA-p23154)通过调节微小RNA375和Krüppel样因子4(KLF4)的表达对人卵巢癌细胞(SKOV3)增殖、侵袭能力的影响。方法:以SKOV3细胞为研究对象,通过转染实验,过表达(过表达组)与抑制(抑制组)细胞中lncRNA-p23154,并设置转染空质粒的SKOV3细胞为对照组,荧光实时定量聚合酶链式反应(qRT-PCR)检测转染效果; CCK8法及Tanswell实验检测各组SKOV3细胞增殖、侵袭情况; qRT-PCR、Western blot检测各组中miR-375及其靶基因KLF4的表达情况。结果:qRT-PCR显示SKOV3细胞中lncRNA-p23154过表达与抑制效果显著(均P<0.05); 与过表达组相比,抑制组SKOV3细胞增殖和侵袭能力均升高(均P<0.05); qRT-PCR、Western blot检测发现,过表达组miR-375在mRNA水平低于抑制组、过表达组KLF4在mRNA和蛋白水平均高于抑制组(均P<0.05)。结论:lncRNA-p23154可能通过miR-375/KLF4途径,影响SKOV3细胞增殖、侵袭能力。
Abstract:
Objective:To observe the effect of long non-coding RNA(lncRNA)p23154 on the proliferation and invasion of ovarian cancer cells(SKOV3)by regulating miR-375 and Krüppel-like factor 4(KLF4).Methods:Over-expression(Over-expression group)and inhibition(inhibition group)of lncRNA p23154 in SKOV3 cells,and set up transfected cells with empty plasmid as control group(NC group).The qRT-PCR was used to detect the transfection effect.CCK8 method and Tanswell experiment were used to detect the proliferation and invasion of SKOV3 cells in each group.Western blot and qRT-PCR were used to detect the expression of miR-375 and its target genes KLF4 in each group.Results:The qRT-PCR showed that lncRNA p23154 overexpression and inhibition were significant(all P<0.05).The proliferation and invasion ability of SKOV3 cells in the inhibition group were both increased(all P<0.05).The level of miR-375 in the overexpression group was lower than that in the inhibition group,and KLF4 mRNA and protein levels in the overexpression group were higher than those in the inhibition group(all P<0.05).Conclusion:LncRNA p23154 may affect the proliferation and invasion of SKOV3 cells through the miR-375/KLF4 pathway.

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备注/Memo

备注/Memo:
基金项目:西安市科技计划项目[20YXYJ0005(6)]
更新日期/Last Update: 2021-11-05