[1]崔 凯,支亚男,王壮壮,等.微小RNA-30对非小细胞肺癌侵袭、转移能力的影响及机制研究[J].陕西医学杂志,2021,50(10):1214-1217，1222.[doi:DOI:10.3969/j.issn.1000-7377.2021.10.009]
　CUI Kai,ZHI Ya'nan,WANG Zhuangzhuang,et al.Effect and mechanism of miR-30 on invasion and metastasis of non-small cell lung cancer[J].,2021,50(10):1214-1217，1222.[doi:DOI:10.3969/j.issn.1000-7377.2021.10.009]
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微小RNA-30对非小细胞肺癌侵袭、转移能力的影响及机制研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年10期
页码:
1214-1217，1222
栏目:
基础研究
出版日期:
2021-10-05

文章信息/Info

Title:
Effect and mechanism of miR-30 on invasion and metastasis of non-small cell lung cancer
作者:
崔 凯; 支亚男; 王壮壮; 公孙鑫; 白峻峰
(西安国际医学中心医院胸腔外科,陕西 西安 710100)
Author(s):
CUI Kai; ZHI Ya'nan; WANG Zhuangzhuang; GONGSUN Xin; BAI Junfeng
(Department of Thoracic Surgery,Xi'an International Medical Center Hospital,Xi'an 710100,China)
关键词:
非小细胞肺癌;  微小RNA-30;  白细胞介素-6;  信号转导和转录活化因子3;  侵袭;  转移
Keywords:
Non-small cell lung cancer;  MiR-30;  IL-6;  STAT3;  Invasion;  Metastasis
分类号:
R 392
DOI:
DOI:10.3969/j.issn.1000-7377.2021.10.009
文献标志码:
A
摘要:
目的:研究微小RNA(miR)-30对非小细胞肺癌(NSCLC)侵袭、转移能力的影响,分析其作用机制。方法:选取30例临床不同病理分期NSCLC患者的癌组织及癌旁组织以及人肺癌细胞系A549细胞和正常肺支气管上皮细胞系HBE细胞为研究对象。比较miR-30在癌组织与癌旁组织、A549细胞与HBE细胞中的表达,分析miR-30对A549细胞迁移、侵袭能力以及白细胞介素-6(IL-6)/信号转导和转录活化因子3(STAT3)信号通路的影响。结果:miR-30在肺癌组织和A549细胞中高表达(均P<0.05)。过表达miR-30的肺癌A549细胞中IL-6/STAT3信号通路分子表达上调,同时细胞的侵袭、迁移能力显著增强,而抑制miR-30表达则可产生相反的结果(均P<0.05)。结论:miR-30能促进NSCLC侵袭和转移能力,其机制可能与激活IL-6/STAT3信号通路有关。
Abstract:
Objective:To study the effect of miR-30 on invasion and metastasis of non-small cell lung cancer(NSCLC)and analyze its mechanism.Methods:The cancer tissues and adjacent tissues of 30 NSCLC patients with different clinical pathological stages,as well as the A549 and HBE cells were selected as the research objects.The expression of miR-30 in cancer tissues and adjacent tissues,A549 and HBE cells were compared.The effects of miR-30 on the migration,invasion and IL-6/STAT3 signaling pathway of A549 cells were analyzed.Results:MiR-30 was highly expressed in lung cancer tissues and A549 cells(all P<0.05).The expression of IL-6/STAT3 signaling pathway was up-regulated in lung cancer A549 cells overexpressing miR-30,and the ability of invasion and migration of A549 cells was significantly enhanced,while the opposite result was obtained by inhibiting the expression of miR-30(all P<0.05).Conclusion:MiR-30 can promote the invasion and metastasis of NSCLC,and its mechanism may be related to the activation of IL-6/STAT3 signaling pathway.

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更新日期/Last Update: 2021-10-08