[1]王 徽,白玉勤.肺腺癌移植瘤裸鼠模型侵袭组织中酪氨酸蛋白激酶受体A7、表皮生长因子受体和Ki67蛋白表达及意义[J].陕西医学杂志,2021,50(9):1060-1063.[doi:DOI:10.3969/j.issn.1000-7377.2021.09.005]
　WANG Hui,BAI Yuqin.Expression and significance of EphA7,EGFR and Ki67 proteins in invasion tissues of lung adenocarcinoma transplanted tumor in nude mice[J].,2021,50(9):1060-1063.[doi:DOI:10.3969/j.issn.1000-7377.2021.09.005]
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肺腺癌移植瘤裸鼠模型侵袭组织中酪氨酸蛋白激酶受体A7、表皮生长因子受体和Ki67蛋白表达及意义
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年9期
页码:
1060-1063
栏目:
基础研究
出版日期:
2021-09-05

文章信息/Info

Title:
Expression and significance of EphA7,EGFR and Ki67 proteins in invasion tissues of lung adenocarcinoma transplanted tumor in nude mice
作者:
王 徽1; 2; 白玉勤3
(1.锦州医科大学,辽宁 锦州 121001; 2.赤峰学院附属医院神经内科,内蒙古 赤峰 024000; 3.赤峰学院第二附属医院 赤峰市肿瘤医院病理科,内蒙古 赤峰 024000)
Author(s):
WANG Hui; BAI Yuqin
(Jinzhou Medical University,Jinzhou 121001,China)
关键词:
肺腺癌;  裸鼠模型;  肿瘤侵袭;  酪氨酸蛋白激酶受体A7;  表皮生长因子受体;  Ki67蛋白
Keywords:
Lung adenocarcinoma;  Nude mouse model;  Tumor invasion;  Ephrin receptor A7;  EGFR;  Ki67 protein
分类号:
R 734.2
DOI:
DOI:10.3969/j.issn.1000-7377.2021.09.005
文献标志码:
A
摘要:
目的:探讨肺腺癌移植瘤裸鼠模型侵袭组织中酪氨酸蛋白激酶受体A7(EphA7)、表皮生长因子受体(EGFR)、Ki67蛋白表达及意义。方法:通过皮下接种肺腺癌A549细胞的方式建立肺腺癌移植瘤裸鼠模型,通过免疫组化染色观察肺腺癌移植瘤侵袭组织与非侵袭组织中EphA7、EGFR、Ki67蛋白表达情况并进行半定量分析。结果:肺腺癌移植瘤侵袭组织与非侵袭组织中EphA7蛋白表达均为阳性,EGFR蛋白表达均为阴性,阳性细胞表达率比较差异无统计学意义(均P>0.05)。肺腺癌移植瘤侵袭组织中Ki67蛋白表达均为阳性,非侵袭组织中Ki67蛋白表达为阴性或弱阳性,侵袭组织阳性细胞表达率高于非侵袭组织(P<0.05)。结论:EphA7、EGFR蛋白表达与肺腺癌侵袭进展无明显关系,Ki67蛋白表达可能会影响肺腺癌侵袭。
Abstract:
Objective:To investigate the expression and significance of ephrin receptor A7(EphA7),epidermal growth factor receptor(EGFR)and Ki67 proteins in invasion tissues of lung adenocarcinoma transplanted tumor in nude mice.Methods:Nude mouse models of lung adenocarcinoma transplanted tumor were established by subcutaneous vaccination of lung adenocarcinoma A549 cells,and immunohistochemical staining was used to observe the expression of EphA7,EGFR,and Ki67 proteins in invasive and non-invasive tissues of lung adenocarcinoma transplanted tumor.Meanwhile,semi-quantitative analysis was conducted.Results:The expression of EphA7 protein was positive in invasive and non-invasive tissues of lung adenocarcinoma transplanted tumor,and the expression of EGFR protein was negative,and there was no significant difference in the expression rate of positive cells(all P>0.05).The expression of Ki67 protein was positive in invasive tissues,and was negative or weakly positive in non-invasive tissues,and the expression rate of positive cells in invasive tissues was higher than that in non-invasive tissues(P<0.05).Conclusion:The expression of EphA7 and EGFR proteins has no obvious relationship with the invasion and progression of lung adenocarcinoma,and the expression of Ki67 protein may affect the invasion of lung adenocarcinoma.

参考文献/References:

[1] Barta JA,Powell CA,Wisnivesky JP.Global epidemiology of lung cancer[J].Ann Glob Health,2019,85(1):8.
[2] Wu D,Xue W,Li X,et al.Long noncoding RNA SNHG14 facilitates colorectal cancer metastasis through targeting EZH2-regulated EPHA7[J].Cell Death Dis,2019,10(7):514.
[3] Wang X,Wang Z.Identification of the soluble EphA7-interacting protein Nicalin as a regulator of EphA7 expression[J].Mol Cell Biochem,2021,476(1):213-220.
[4] Sigismund S,Avanzato D,Lanzetti L.Emerging functions of the EGFR in cancer[J].Mol Oncol,2018,12(1):3-20.
[5] Harrison PT,Vyse S,Huang PH.Rare epidermal growth factor receptor(EGFR)mutations in non-small cell lung cancer[J].Semin Cancer Biol,2020,61:167-179.
[6] Kreipe H.Ki67:Biological intertumor variance versus variance of assay[J].Pathologe,2018,39(2):272-277.
[7] 白玉勤,吴 宝,张 龙,等.非小细胞肺癌移植瘤侵袭组织中血清蛋白的分布[J].癌变?畸变?突变,2018,30(2):54-57.
[8] Leonard CE,Baydyuk M,Stepler MA,et al.EphA7 isoforms differentially regulate cortical dendrite development[J].PLoS One,2020,15(12):e0231561.
[9] 刘 正,王 静,沈 伟,等.EphA7在骨肉瘤中的表达及对骨肉瘤MG-63细胞增殖、迁移和凋亡的影响[J].中国癌症杂志,2019,29(3):18-24.
[10] 陈 放,熊 玮,杜杨春,等.EphrinA2和EphA7在前列腺癌中的表达及临床意义[J].临床误诊误治,2018,31(9):65-69.
[11] 王 敬,黄卫东,柳 锋.口腔鳞癌中EphA7表达及其与肿瘤干细胞的关系[J].临床肿瘤学杂志,2018,23(2):127-131.
[12] 金 红,莫 迪,李梦雨,等.抑制EphA7表达对非小细胞肺癌细胞迁移和侵袭能力的影响[J].中国老年学杂志,2020,40(1):164-167.
[13] Yoshida Y,Kabara M,Kano K,et al.Capillary-resident EphA7+ pericytes are multipotent cells with anti-ischemic effects through capillary formation[J].Stem Cells Transl Med,2020,9(1):120-130.
[14] Arnold LL,Cecchini A,Stark DA,et al.EphA7 promotes myogenic differentiation via cell-cell contact[J].Elife,2020,21(9):53689.
[15] Eskilsson E,Røsland GV,Solecki G,et al.EGFR heterogeneity and implications for therapeutic intervention in glioblastoma[J].Neuro Oncol,2018,20(6):743-752.
[16] Sabbah DA,Hajjo R,Sweidan K.Review on epidermal growth factor receptor(EGFR)structure,signaling pathways,interactions,and recent updates of EGFR inhibitors[J].Curr Top Med Chem,2020,20(10):815-834.
[17] 刘 丽,杨 洋,张万里,等.厄洛替尼治疗晩期EGFR敏感突变肺腺癌对血管内皮功能及机体慢性炎症反应的影响[J].陕西医学杂志,2018,47(10):1275-1277.
[18] Leonetti A,Sharma S,Minari R,et al.Resistance mechanisms to osimertinib in EGFR-mutated non-small cell lung cancer[J].Br J Cancer,2019,121(9):725-737.
[19] 赵 敏,田 畅,邸 鑫,等.RBM5、EGFR和KRAS在非小细胞肺癌中的表达及其意义[J].中国实验诊断学,2019,23(2):303-308.
[20] Klöppel G,Rosa S.Ki67 labeling index:Assessment and prognostic role in gastroentero pancreatic neuroendocrine neoplasms[J].Virchows Arch,2018,472(3):341-349.

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备注/Memo

备注/Memo:
基金项目:内蒙古自治区高等学校科学研究项目(NJZZ18202)
更新日期/Last Update: 2021-09-06