[1]田蕴霖,白淑玮,邵 娟.α1-抗胰蛋白酶抑制高糖诱导大鼠视网膜Müller细胞凋亡实验研究[J].陕西医学杂志,2021,50(8):907-909,933.[doi:DOI:10.3969/j.issn.1000-7377.2021.08.001]
　TIAN Yunlin,BAI Shuwei,SHAO Juan.Inhibitory effect of α1-antitrypsin on apoptosis of rat retinal Müller cells induced by high glucose[J].,2021,50(8):907-909,933.[doi:DOI:10.3969/j.issn.1000-7377.2021.08.001]
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α1-抗胰蛋白酶抑制高糖诱导大鼠视网膜Müller细胞凋亡实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年8期
页码:
907-909,933
栏目:
基础研究
出版日期:
2021-08-05

文章信息/Info

Title:
Inhibitory effect of α1-antitrypsin on apoptosis of rat retinal Müller cells induced by high glucose
作者:
田蕴霖; 白淑玮; 邵 娟
(西安市人民医院 西安市第四医院眼科,陕西 西安 710004)
Author(s):
TIAN Yunlin; BAI Shuwei; SHAO Juan
(Department of Ophthalmology,Xi'an People's Hospital,Xi'an 710004,China)
关键词:
α1-抗胰蛋白酶;  高糖;  视网膜;  Müller细胞;  细胞凋亡;  大鼠
Keywords:
α1-antitrypsin;  High glucose;  Retina;  Müller cells;  Apoptosis;  Rat
分类号:
R 774.1
DOI:
DOI:10.3969/j.issn.1000-7377.2021.08.001
文献标志码:
A
摘要:
目的:探讨α1-抗胰蛋白酶(AAT)抑制高糖诱导大鼠视网膜Müller细胞凋亡的效果与机制。方法:大鼠视网永生化膜Müller细胞系在高糖培养基上培养,分为三组:模型组、试验A组、试验B组,分别加入不同浓度的外源性AAT 100 μl(加药浓度分别为0、10、100 pg/ml),处理不同时间点换回高糖培养基进行培养。采用MTT法检测细胞增殖,流式细胞仪检测细胞凋亡,Transwell小室法检测细胞侵袭,Western blot法检测蛋白表达。结果:处理3、6 h后,试验A组、试验B组的细胞增殖指数、细胞侵袭指数均高于对照组(均P<0.05),试验B组高于试验A组(P<0.05)。处理3、6 h后,试验A组、试验B组的细胞凋亡指数、信号转导及转录活化因子3(STAT3)蛋白相对表达水平均低于对照组(均P<0.05),试验B组低于试验A组(P<0.05)。结论:AAT可抑制高糖诱导大鼠视网膜Müller细胞的凋亡,促进细胞增殖与侵袭,其作用机制可能与抑制STAT3表达有关。
Abstract:
Objective:To investigate the effect and mechanism of α1-antitrypsin(AAT)on inhibiting the apoptosis of rat retinal Müller cells induced by high glucose.Methods:The rat visual net immortalized membrane Müller r cell line were cultured on high glucose medium and divided into three groups:model group,test A group,and test B group.The three groups were added with different concentrations of exogenous AAT 100 μl(the concentration was 0,10,100 pg/ml,respectively).The treatment were changed to high glucose medium for culture at different time points.MTT method was used to detect cell proliferation,and flow cytometry was used to detect cell apoptosis,and Transwell chamber method was used to detect cell invasion,and Western blot method was used to detect protein expression.Results:3 and 6 hours after treatment,the cell proliferation index and cell invasion index of test A group and test B group were higher than those of control group,and test B group was higher than test A group(all P<0.05); the relative expression levels of apoptosis index,signal transducers and activators of transcription 3(STAT3)protein in test A group and test B group were lower than those in the control group,and test B group was lower than test A group(all P<0.05).Conclusion:AAT can inhibit the apoptosis of rat retinal Müller cells induced by high glucose,and promote cell proliferation and invasion.Its mechanism may be related to the inhibition of STAT3 expression.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81302198)
更新日期/Last Update: 2021-08-05