[1]马 骞,朱 军,蔡梦珊,等.基于exoRBase外泌体数据库结直肠癌竞争性内源RNA网络构建及富集分析[J].陕西医学杂志,2021,50(7):797-801.[doi:DOI:10.3969/j.issn.1000-7377.2021.07.007]
　MA Qian,ZHU Jun,CAI Mengshan,et al.Construction and enrichment analysis of competing endogenous RNA network for colorectal cancer based on the exoRBase database[J].,2021,50(7):797-801.[doi:DOI:10.3969/j.issn.1000-7377.2021.07.007]
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基于exoRBase外泌体数据库结直肠癌竞争性内源RNA网络构建及富集分析
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年7期
页码:
797-801
栏目:
临床研究
出版日期:
2021-07-05

文章信息/Info

Title:
Construction and enrichment analysis of competing endogenous RNA network for colorectal cancer based on the exoRBase database
作者:
马 骞1; 2; 朱 军2; 蔡梦珊1; 郝 俊2; 章屹然3; 陈 睿2
(1.西安医学院,陕西 西安710021; 2.空军军医大学西京医院,陕西 西安710032; 3.空军军医大学基础医学院,陕西 西安 710032)
Author(s):
MA Qian; ZHU Jun; CAI Mengshan; HAO Jun; ZHANG Yiran; CHEN Rui
(Xi'an Medical University,Xi'an 710021,China)
关键词:
结直肠癌;  新型诊断标志物;  外泌体;  竞争性内源RNA;  富集分析;  液体活检
Keywords:
Colorectal cancer;  New diagnostic marker;  Exosomes;  Competing endogenous RNA;  Enrichment analysis;  Liquid biopsy
分类号:
R 735.3
DOI:
DOI:10.3969/j.issn.1000-7377.2021.07.007
文献标志码:
A
摘要:
目的:基于结直肠癌(CRC)患者和正常人血浆外泌体RNA筛选差异性RNA,构建竞争性内源RNA(ceRNA)网络,并进行富集分析。方法:利用exoRBase数据库中CRC患者和正常人血浆标本外泌体RNA[包括信使RNA(mRNA)、长链非编码RNA(lncRNA)、环状RNA(circRNA)]表达谱,依据ceRNA假说构建CRC发生相关的ceRNA调控网络,并对网络中mRNA进行基因本体论(GO)注释和京都基因与基因组百科全书(KEGG)通路富集分析,探索ceRNA网络的潜在功能。结果:共筛选出125个差异表达的mRNAs(DEmRNAs)、53个差异表达的lncRNAs(DElncRNAs)和81个差异表达的circRNAs(DEcircRNAs),并与其对应的miRNA预测数据构建ceRNA调控网络,再对ceRNA网络的25个共同差异表达mRNA进行富集分析。GO注释富集分析显示,CRC ceRNA网络中的外泌体mRNA与癌症相关通路主要富集在GTP结合蛋白RAN通路。KEGG通路富集分析显示,调控网络中差异表达的mRNAs主要富集于促分裂原活化蛋白激酶(MAPK)和血管内皮生长因子(VEGF)信号通路。结论:本研究确定了CRC发生相关的外泌体RNA并构建了相应的ceRNA调控网络,为进一步研究CRC转移进展提供了分子基础,同时为CRC的早期诊断提供了新型的液体活检生物学标志物。
Abstract:
Objective:To screen differential RNA based on plasma exosomal RNA of patients with colorectal cancer(CRC)and normal people,and to construct a network of competing endogenous RNA(ceRNA),and to perform enrichment analysis.Methods:Based on the ceRNA hypothesis,the CRC related ceRNA regulatory network was constructed by using exoRBase database of CRC patients and normal human plasma samples exosomal RNA [including messenger RNA(mRNA),long non-coding RNA(lncRNA),circular RNA(circRNA)] expression profile,and GO annotation and KEGG pathway enrichment analysis were performed on mRNA in the network to explore the potential functions of the ceRNA network.Results:A total of 125 DEmRNAs,53 DElncRNAs and 81 DEcircRNAs were screened out,and combined with their corresponding miRNAs prediction data to construct a ceRNA regulatory network,and then the enrichment analysis of 25 mRNAs in the ceRNA network was performed.GO enrichment analysis showed that the exosomal mRNA and cancer-related pathways in the CRC ceRNA network were mainly enriched in the GTP-binding protein RAN pathway.KEGG enrichment analysis showed that the DEmRNAs in the regulatory network were mainly enriched in mitogen-activated protein kinase(MAPK)and vascular endothelial growth factor(VEGF)signaling pathways.Conclusion:This study identified the exosomal RNA related to the occurrence of CRC and constructed the corresponding ceRNA regulatory network,which provides a molecular basis for further research on the metastasis and progress of CRC,and provides a new type of liquid biopsy biological marker for the early diagnosis of CRC.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助面上项目(81672751)
更新日期/Last Update: 2021-07-05