[1]李正民,李江静,庄莹莹,等.益生菌对脂多糖诱发大鼠脑内神经炎症及认知功能障碍保护作用的实验研究[J].陕西医学杂志,2021,50(7):771-775.[doi:DOI:10.3969/j.issn.1000-7377.2021.07.001]
　LI Zhengmin,LI Jiangjing,ZHUANG Yingying,et al.Effects of probiotics on brain neuroinflammation and cognitive dysfunction induced by lipopolysaccharide in rats[J].,2021,50(7):771-775.[doi:DOI:10.3969/j.issn.1000-7377.2021.07.001]
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益生菌对脂多糖诱发大鼠脑内神经炎症及认知功能障碍保护作用的实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年7期
页码:
771-775
栏目:
基础研究
出版日期:
2021-07-05

文章信息/Info

Title:
Effects of probiotics on brain neuroinflammation and cognitive dysfunction induced by lipopolysaccharide in rats
作者:
李正民; 李江静; 庄莹莹; 闫 辉; 刘伟娜; 屈丽虹
(空军军医大学唐都医院麻醉科,陕西 西安 710038)
Author(s):
LI Zhengmin; LI Jiangjing; ZHUANG Yingying; YAN Hui; LIU Weina; QU Lihong
(Department of Anesthesiology,Tangdu Hospital,Air Force Medical University,Xi'an 710038,China)
关键词:
认知功能障碍;  益生菌;  脂多糖;  炎症因子;  大鼠
Keywords:
Cognitive dysfunction;  Probiotics;  Lipopolysaccharide;  Inflammatory cytokines;  Rats
分类号:
R 741
DOI:
DOI:10.3969/j.issn.1000-7377.2021.07.001
文献标志码:
A
摘要:
目的:研究益生菌对脂多糖(LPS)诱发大鼠脑内神经炎症及认知功能障碍的保护作用,为神经炎症以及神经退行性病变的防治研究提供进一步的实验基础。方法:成年雄性SD大鼠随机分为四组:对照组、LPS组、LPS+益生菌组、益生菌组。通过Morris水迷宫观察大鼠空间学习记忆能力,通过ELISA 实验方法,研究大鼠大脑皮层组织肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)以及诱导型一氧化氮合酶(iNOS)的表达变化。通过蛋白免疫印迹Western blot和PCR研究大鼠脑皮质脑源性神经营养因子(BDNF)的表达。结果:水迷宫结果显示:LPS可致大鼠认知功能障碍,学习记忆能力下降,口服益生菌可改善认知能力。ELISA结果显示:与对照组相比,LPS组大鼠大脑皮层组织TNF-α、IL-1β、IL-6、iNOS水平升高,差异有统计学意义(P<0.05),而LPS+益生菌组与LPS组相比,IL-1β、IL-6、iNOS水平降低,差异有统计学意义(均P<0.05)。结论:益生菌可减轻LPS所致的神经炎症,改善LPS所致的大鼠认知功能障碍。
Abstract:
Objective:To investigate the protective effect of probiotics on brain neuroinflammation and cognitive dysfunction induced by lipopolysaccharide(LPS)in rats,and to provide a further laboratory basis for the prevention and treatment of neuroinflammation and neurodegenerative diseases.Methods:Adult male SD rats were randomly divided into control group,LPS group,LPS+probiotics group and probiotics group.The spatial learning and memory ability of rats was observed by Morris water maze.The expression levels of TNF-α,interleukin-1β(IL-1β),interleukin-6(IL-6)and inducible nitric oxide synthase(iNOS)in the cerebral cortex of rats were investigated by ELISA.The expression of brain-derived neurotrophic factor(BDNF)in the cerebral cortex of rats was studied by Western blot and PCR.Results:The results of Morris water maze showed that LPS could cause cognitive dysfunction and decrease of learning and memory ability in rats,and oral probiotics could change this cognitive ability.Compared with the control group,the levels of TNF-α,IL-1β,IL-6 and iNOS in cerebral cortex were increased in LPS group,and the levels of IL-1β,IL-6 and iNOS in LPS+probiotics group were lower than those in LPS group(all P<0.05).Western blot and PCR results showed that LPS could decrease the expression of BDNF in the cerebral cortex of rats,and oral probiotics could increase the expression of BDNF.Conclusion:Probiotics can alleviate LPS-induced neuroinflammation,increase the expression of BDNF in the cerebral cortex,and improve cognitive dysfunction in rats.

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学重大基础研究项目(2016ZDJC-16)
更新日期/Last Update: 2021-07-05