[1]孙玉敏,李 绒.冠心病患者血清miR-21、miR-29a表达水平与心电图QRS波时限相关性分析[J].陕西医学杂志,2021,50(6):713-716.[doi:DOI:10.3969/j.issn.1000-7377.2021.06.018]
　SUN Yumin,LI Rong.Correlation analysis of serum miR-21,miR-29a and QRS duration in patients with coronary heart disease[J].,2021,50(6):713-716.[doi:DOI:10.3969/j.issn.1000-7377.2021.06.018]
点击复制

冠心病患者血清miR-21、miR-29a表达水平与心电图QRS波时限相关性分析
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年6期
页码:
713-716
栏目:
临床研究
出版日期:
2021-06-05

文章信息/Info

Title:
Correlation analysis of serum miR-21,miR-29a and QRS duration in patients with coronary heart disease
作者:
孙玉敏1; 李 绒2
(1.延安市人民医院功能科,陕西 延安 716000; 2.陕西省核工业二一五医院功能科,陕西 咸阳 712000)
Author(s):
SUN Yumin; LI Rong
(Department of Function,Yan'an People's Hospital,Yan'an 716000,China)
关键词:
冠心病;  微小RNA-21;  微小RNA-29a;  心电图;  QRS波时限;  相关性
Keywords:
CHD;  MicroRNA-21;  MicroRNA-29a;  Electrocardiogram;  QRS duration;  Correlation
分类号:
R 541.4
DOI:
DOI:10.3969/j.issn.1000-7377.2021.06.018
文献标志码:
A
摘要:
目的:探究微小RNA-21(miR-21)、微小RNA-29a(miR-29a)在冠心病(CHD)患者血清中的水平及与心电图QRS波时限的相关性。方法:收集162例CHD患者作为CHD组,并选取同期166例健康体检者作为对照组。采用实时荧光定量PCR(qRT-PCR)法检测两组血清miR-21、miR-29a水平。以酶联免疫吸附法(ELISA)测定基质金属蛋白酶-2(MMP-2)、肿瘤坏死因子-α(TNF-α)水平。使用心电图机检测QRS波时限。分析CHD患者血清miR-21、miR-29a、QRS波时限与MMP-2、TNF-α,以及miR-21、miR-29a与QRS波时限的相关性。采用Logistic回归模型分析CHD影响因素。结果:CHD组三酰甘油、总胆固醇、低密度脂蛋白、miR-21、miR-29a、QRS波时限、MMP-2、TNF-α水平高于对照组,高密度脂蛋白低于对照组(均P<0.05)。CHD患者血清miR-21、miR-29a、QRS波时限与MMP-2、TNF-α,以及miR-21、miR-29a表达水平与QRS波时限呈正相关(均P<0.05)。miR-21、miR-29a、QRS波时限、MMP-2、TNF-α均是影响CHD发生的危险因素(均P<0.05)。结论:CHD患者血清miR-21、miR-29a呈高表达,两者均与心电图QRS波时限呈正相关。miR-21、miR-29a可能与心功能不全相互影响,从而在CHD发病过程中发挥重要作用。
Abstract:
Objective:To investigate the levels of serum microRNA-21(miR-21)and microRNA-29a(miR-29a)in patients with coronary heart disease(CHD)and their correlations with QRS duration of ECG.Methods:162 CHD patients were collected as the CHD group,and 166 healthy physical examination patients during the same period were selected as the control group.The levels of serum miR-21 and miR-29a were detected by real-time fluorescence quantitative PCR(qRT-PCR).The levels of matrix metalloproteinase-2(MMP-2)and tumor necrosis factor-α(TNF-α)were measured by enzyme-linked immunosorbent assay(ELISA).The QRS duration was measured by electrocardiograph in CHD patients.The correlations between serum miR-21,miR-29a,QRS duration and MMP-2,TNF-α,between miR-21,miR-29a and QRS duration were analyzed.The influencing factors of CHD were analyzed by Logistic regression.Results:The levels of triglyceride,total cholesterol,low density lipoprotein,miR-21,miR-29a,QRS duration,MMP-2 and TNF-α in the CHD group were higher than those in the control group(all P<0.05),and high density lipoprotein was lower than that in the control group(P<0.05).In CHD patients,the levels of serum miR-21,miR-29a and QRS duration were positively correlated with the levels of MMP-2 and TNF-α,and the levels of miR-21 and miR-29a were positively correlated with QRS duration(all P<0.05).The levels of miR-21,miR-29a,QRS duration,MMP-2 and TNF-α were all risk factors for CHD(all P<0.05).Conclusion:The levels of serum miR-21 and miR-29a in CHD patients are high,and both of them are positively correlated with QRS duration.The miR-21 and miR-29a may interact with cardiac insufficiency and play an important role in the pathogenesis of CHD.

参考文献/References:

[1] 陈曼丽,宫 蕊,齐莉莉,等.丹红注射液联合麝香保心丸治疗冠心病心绞痛疗效及对患者NF-κB、GMP-140的影响[J].陕西中医,2020,41(3):321-323.
[2] 王晓萍,王志荣.心电图联合心脏彩超诊断冠心病心力衰竭病情严重程度的价值研究[J].中国急救复苏与灾害医学杂志,2019,14(11):1038-1041.
[3] Kaudewitz D,Zampetaki A,Mayr M.MicroRNA biomarkers for coronary artery disease[J].Curr Atheroscler Rep,2015,17(12):70-83.
[4] Liu H,Yang N,Fei Z,et al.Analysis of plasma miR-208a and miR-370 expression levels for early diagnosis of coronary artery disease[J].Biomed Rep,2016,5(3):332-336.
[5] Han H,Qu G,Han C,et al.MiR-34a,miR-21 and miR-23a as potential biomarkers for coronary artery disease:A pilot microarray study and confirmation in a 32 patient cohort[J].Exp Mol Med,2015,47(2):138-143.
[6] Liu CZ,Zhong Q,Huang YQ.Elevated plasma miR-29a levels are associated with increased carotid intima-media thickness in atherosclerosis patients[J].Tohoku J Exp Med,2017,241(3):183-188.
[7] 刘万车.冠状动脉粥样硬化性心脏病的命名及诊断标准[J].中国社区医师,1989,12(1):11-13.
[8] 孙时淼,张 涛.冠心病患者术前血清超氧化物歧化酶和微小RNA-29b表达及与冠脉CTA钙化积分相关性研究[J].陕西医学杂志,2020,49(7):855-857,903.
[9] Panganiban RP,Wang Y,Howrylak J,et al.Circulating microRNAs as biomarkers in patients with allergic rhinitis and asthma[J].J Allergy Clin Immunol,2016,137(5):1423-1432.
[10] Buie JNJ,Goodwin AJ,Cook JA,et al.The role of miRNAs in cardiovascular disease risk factors[J].Atherosclerosis,2016,254(9):271-281.
[11] Li Z,Song Y,Liu L,et al.MiR-199a impairs autophagy and induces cardiac hypertrophy through mTOR activation[J].Cell Death Differ,2017,24(7):1205-1213.
[12] Velle-Forbord T,Eidlaug M,Debik J,et al.Circulating microRNAs as predictive biomarkers of myocardial infarction:Evidence from the HUNT study[J].Atherosclerosis,2019,289(7):1-7.
[13] Wang S,He W,Wang C.MiR-23a regulates the vasculogenesis of coronary artery disease by targeting epidermal growth factor receptor[J].Cardiovasc Ther,2016,34(4):199-208.
[14] Sygitowicz G,Tomaniak M,Blaszczyk O,et al.Circulating microribonucleic acids miR-1,miR-21 and miR-208a in patients with symptomatic heart failure:Preliminary results[J].Arch Cardiovasc Dis,2015,108(12):634-642.
[15] Yan M,Chen C,Gong W,et al.MiR-21-3p regulates cardiac hypertrophic response by targeting histone deacetylase-8[J].Cardiovasc Res,2015,105(3):340-352.
[16] Zhang S,Yin Z,Dai FF,et al.MiR-29a attenuates cardiac hypertrophy through inhibition of PPARδ expression[J].J Cell Physiol,2019,234(8):13252-13262.
[17] 仇晓敏,胡晓晟,蒲建芬,等.冠心病心力衰竭患者QRS波时限与血浆Hcy的相关性分析[J].中华全科医学,2016,14(7):1134-1137.
[18] 白雨辰.冠心病患者血清Hcy水平与心电图QRS波的相关性及意义[J].辽宁医学杂志,2020,34(1):37-40.
[19] 金晓宇,王 阳,栗印军,等.冠心病患者外周血单核细胞中EMMPRIN、MMP-2表达变化及与冠状动脉粥样硬化的关系[J].山东医药,2015,55(17):20-23.
[20] 卢 杰,付 鑫,张继红,等.RBP4/TNF-α在冠心病患者血浆中的表达情况及与冠脉病变程度的相关性研究[J].安徽医药,2017,18(11):1998-2001.

相似文献/References:

[1]李艳艳,贾 伟△.24h动态心电图在2型糖尿病合并冠心病患者心律诊断中的价值分析[J].陕西医学杂志,2019,(1):61.
[2]汤克虎,高峻峰,任尽平.药物涂层球囊在糖尿病患者冠状动脉小血管病变治疗中的应用研究*[J].陕西医学杂志,2019,(4):432.
[3]马党捐 ,康彦智 ,柴茂林.64排128层螺旋CT冠状动脉成像在冠心病患者诊断中的价值研究*[J].陕西医学杂志,2019,(5):624.
　MA Dangjuan,KANG Yanzhi,CHAI Maolin..Value analysis of 64rows 128slice spiral CT coronary angiography in the diagnosis of patients with coronary heart disease[J].,2019,(6):624.
[4]沈 琳,马文苑.曲美他嗪联合氯吡格雷治疗冠心病心绞痛疗效及对患者心电图、血清超敏C反应蛋白和五聚素3的影响[J].陕西医学杂志,2019,(6):794.
　SHEN Lin,MA Wenyuan.ElectrocardiogramRoom,Therapeutic Effect ofTrimetazidine Combined with Clopidogrel on Angina Pectoris and its Hs-CRP[J].,2019,(6):794.
[5]杨延洁,周慧龙△.不同双抗血小板疗程对冠心病合并房颤PCI术后患者远期预后的影响*[J].陕西医学杂志,2019,(12):1653.
[6]魏 勇,梁 琪,左浩源,等.右美托咪定预处理对冠心病非心脏手术患者心肌的保护作用*[J].陕西医学杂志,2019,(12):1706.
　WEI Yong,LIANG Qi,ZUO Haoyuan,et al.Protective effect of dexmedetomidine preconditioning on myocardium in patients with coronary heart disease undergoing nonheart surgery[J].,2019,(6):1706.
[7]刘玉珊.心外膜脂肪定量及可溶性血栓调节蛋白与冠心病病变程度相关性分析[J].陕西医学杂志,2020,49(1):44.
　LIU Yushan..Correlation between the distribution of epicardial adipose tissue and soluble thrombomodulin and the severity of coronary heart disease[J].,2020,49(6):44.
[8]赵 博,王银谦,王红燕.巨噬细胞移动抑制因子与冠心病关系Meta分析[J].陕西医学杂志,2020,49(1):125.
[9]王亚平,彭春艳,朱名安Δ.冠心病合并代谢综合征患者固醇调节元件结合蛋白表达水平与脂代谢指标相关性研究*[J].陕西医学杂志,2020,49(4):419.
　WANG Yaping,PENG Chunyan,ZHU Mingan..Correlation between the levels of SREBPs and the indicators of lipid metabolism in patients with coronary heart disease complicated with metabolic syndrome[J].,2020,49(6):419.
[10]南格利.冠心病颈动脉超声参数与斑块稳定性及炎症反应水平相关性分析[J].陕西医学杂志,2020,49(4):453.
　NAN Geli..Correlation between carotid ultrasound parameters and plaque stability and inflammatory response in patients with coronary heart disease[J].,2020,49(6):453.

备注/Memo

备注/Memo:
基金项目:陕西省科技计划项目(2018SF-256)
更新日期/Last Update: 2021-06-07