[1]舒 放,王海峰,薛飞肖,等.乙型肝炎病毒S基因不同区域嵌合丙型肝炎病毒中和抗原表位病毒样颗粒实验研究[J].陕西医学杂志,2021,50(6):660-663.[doi:DOI:10.3969/j.issn.1000-7377.2021.06.005]
　SHU Fang,WANG Haifeng,XUE Feixiao,et al.Comparison of chimeric virus-like particles of HCV neutralizing epitopes in different regions of HBV S gene[J].,2021,50(6):660-663.[doi:DOI:10.3969/j.issn.1000-7377.2021.06.005]
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乙型肝炎病毒S基因不同区域嵌合丙型肝炎病毒中和抗原表位病毒样颗粒实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年6期
页码:
660-663
栏目:
基础研究
出版日期:
2021-06-05

文章信息/Info

Title:
Comparison of chimeric virus-like particles of HCV neutralizing epitopes in different regions of HBV S gene
作者:
舒 放1; 王海峰1; 薛飞肖1; 吕海港2
(1.西北大学附属医院 西安市第三医院检验科,陕西 西安 710018; 2.西安交通大学附属红会医院麻醉科,陕西 西安 710054)
Author(s):
SHU Fang; WANG Haifeng; XUE Feixiao; LYU Haigang
(Department of Clinical Laboratory,Affiliated Hospital of Northwest University,Xi'an 710018,China)
关键词:
乙型肝炎病毒;  丙型肝炎病毒;  病毒样颗粒;  中和抗原;  表达载体;  胞外区
Keywords:
HBV;  HCV;  Virus-like particle;  Neutralizing antigen;  Expression vector;  Extracellular region
分类号:
R 392.1
DOI:
DOI:10.3969/j.issn.1000-7377.2021.06.005
文献标志码:
A
摘要:
目的:串联丙型肝炎病毒(HCV)中和抗原表位嵌合在乙型肝炎病毒(HBV)S基因不同区域并对形成的病毒样颗粒(VLP)进行比较。方法:选取3种HCV中和抗原表位进行串联,分别嵌合在HBV S基因亲水区和氨基端胞外区,构建两种表达载体pCI-NEmS和pCI-S1EmS2。通过10次重复实验转染人胚胎肾细胞293T(HEK293T细胞)后收集培养上清,经过浓缩、纯化制备VLP-NEmS和VLP-S1EmS2。采用电化学发光法对两种VLP进行乙型肝炎表面抗原(HBsAg)定量检测,分析两种VLP含量均值的差异。结果:VLP-S1EmS2的HBsAg均值低于VLP-HBS(P<0.05),而VLP-NEmS与VLP-HBS比较差异无统计学意义(P=0.157)。结论:胞外区嵌合VLP-NEmS与亲水区VLP-S1EmS2浓度结果有区别,胞外区含量较高。
Abstract:
Objective:To construct HCV series neutralizing epitope chimera in different regions of HBV S gene,and to compare the formed virus-like particle(VLP).Methods:Three HCV neutralizing epitopes were selected to form series and respectively chimerized in the hydrophilic region and the extracellular region of amino terminal with the HBV S gene to construct two expression vectors pCI-NEmS and pCI-S1EmS2.HEK293T cells were transfected and the culture supernatant was collected and concentrated and purified to prepare VLP-NEmS and VLP-S1EmS2 for 10 times repeated experiments.The electrochemiluminescence method was used to quantitatively detect the HBsAg of the two VLPs.Results:The mean value of HBsAg of VLP-S1EmS2 was lower than that of VLP-HBS(P<0.05),but there was no significant difference between VLP-NEmS and VLP-HBS(P=0.157).Conclusion:The concentration of chimeric VLP-NEmS in the extracellular region is higher than that of VLP-S1EmS2 in the hydrophilic region.

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备注/Memo

备注/Memo:
基金项目:陕西省重点研发计划项目(2019SF-156)
更新日期/Last Update: 2021-06-07