[1]王松海,康 超,谢燕华,等.红景天苷通过抑制线粒体自噬通路减轻1-甲基-4-苯基吡啶离子诱导的PC12细胞凋亡实验研究[J].陕西医学杂志,2021,50(6):652-656.[doi:DOI:10.3969/j.issn.1000-7377.2021.06.003]
　WANG Songhai,KANG Chao,XIE Yanhua,et al.Experimental study of salidroside attenuating MPP+-induced apoptosis of PC12 cells through inhibiting mitochondrial autophagy pathway[J].,2021,50(6):652-656.[doi:DOI:10.3969/j.issn.1000-7377.2021.06.003]
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红景天苷通过抑制线粒体自噬通路减轻1-甲基-4-苯基吡啶离子诱导的PC12细胞凋亡实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年6期
页码:
652-656
栏目:
基础研究
出版日期:
2021-06-05

文章信息/Info

Title:
Experimental study of salidroside attenuating MPP+-induced apoptosis of PC12 cells through inhibiting mitochondrial autophagy pathway
作者:
王松海; 康 超; 谢燕华; 陈 捷; 刘 楠
(陕西省中医医院肿瘤科,陕西 西安 710003)
Author(s):
WANG Songhai; KANG Chao; XIE Yanhua; CHEN Jie; LIU Nan
(Department of Oncology,Shaanxi Provincial Hospital of Chinese Medicine,Xi'an 710003,China)
关键词:
红景天苷;  线粒体自噬通路;  帕金森病;  凋亡;  线粒体膜电位
Keywords:
Salidroside;  Mitochondrial autophagy pathway;  Parkinson's disease;  Apoptosis;  Mitochondrial membrane potential
分类号:
R 742.5
DOI:
DOI:10.3969/j.issn.1000-7377.2021.06.003
文献标志码:
A
摘要:
目的:研究红景天苷(Sal)对1-甲基-4-苯基吡啶离子(MPP+)诱导的PC12细胞凋亡的影响及其可能机制。方法:PC12细胞培养后分为四组,对照组不加任何实验受试物,单纯药物组加入100 μmol/L Sal。MPP+模型组加入MPP+(终浓度500 μmol/L),不同剂量 Sal组加入终浓度10、100 μmol/L Sal。MTT法检测PC12细胞活性,流式细胞仪检测细胞凋亡情况,荧光染料罗丹明123(Rh123)检测线粒体膜电位(MMP)变化,Western blot检测LC-3β、Beclin1、PINK1、Parkin、β-actin蛋白表达情况。结果:MPP+(500 μmol/L)作用于PC12细胞24 h后,与对照组比较,细胞存活率下降,凋亡细胞比例增加,MMP降低,细胞内LC-3β、Beclin1表达增多且PINK1、Parkin表达减少(均P<0.05)。Sal(10、100 μmol/L)预处理24 h后,与MPP+模型组比较,细胞存活率增加,凋亡细胞比例下降,MMP升高,细胞内LC-3β、Beclin1表达减少且PINK1、Parkin表达增加(均P<0.05)。结论:Sal对MPP+诱导的PC12细胞凋亡具有浓度依赖性的抑制作用,其机制可能与激活线粒体自噬通路有关。
Abstract:
Objective:To study the effect of salidroside(Sal)on the apoptosis of PC12 cells induced by 1-methyl-4-phenylpyridine ion(MPP+)and its possible mechanism.Methods:After culturing PC12 cells,they were divided into four groups.The control group did not add any test substance.The drug-only group was added with 100 μmol/L Sal.The MPP+ model group was added with MPP+(final concentration 500 μmol/L).The different dose Sal group was added with final concentration of 10,100 μmol/L Sal.MTT method was used to detect PC12 cells viability,and flow cytometry was used to detect cell apoptosis,and fluorescent dye rhodamine 123(Rh123)was used to detect mitochondrial membrane potential(MMP)changes,and Western blot was used to detect expression of LC-3β,Beclin1,PINK1,Parkin,and β-actin protein.Results:After MPP+(500 μmol/L)acted on PC12 cells for 24 hours,compared with the control group,the cell survival rate decreased,the proportion of apoptotic cells increased,MMP decreased,the expression of LC-3β and Beclin1 in the cells increased,and the expression of PINK1 and Parkin decreased(all P<0.05).After pretreatment with Sal(10,100 μmol/L)for 24 hours,compared with the MPP+ model group,the cell survival rate increased,the proportion of apoptotic cells decreased,MMP increased,the expression of LC-3β and Beclin1 decreased,and the expression of PINK1 and Parkin increased(all P<0.05).Conclusion:Sal plays a possible neuroprotective role in MPP+-induced apoptosis of PC12 cells by a concentration-dependent manner,and the mechanism of may be related to activate mitochondrial autophagy pathway.

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备注/Memo

备注/Memo:
基金项目:西安市科技局基金资助项目[2017122SF/YX016(8)]
更新日期/Last Update: 2021-06-07