[1]李长超,柴艳梅,刘 丹,等.类风湿关节炎患者血清IL-13水平表达及对外周血单个核细胞中Th17细胞亚群分化的影响[J].陕西医学杂志,2021,50(2):197-200.[doi:DOI:10.3969/j.issn.1000-7377.2021.02.018]
　LI Changchao,CHAI Yanmei,LIU Dan,et al.Expression of serum IL-13 in patients with rheumatoid arthritis and its effect on differentiation of Th17 cell subsets in PBMC[J].,2021,50(2):197-200.[doi:DOI:10.3969/j.issn.1000-7377.2021.02.018]
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类风湿关节炎患者血清IL-13水平表达及对外周血单个核细胞中Th17细胞亚群分化的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年2期
页码:
197-200
栏目:
临床研究
出版日期:
2021-02-05

文章信息/Info

Title:
Expression of serum IL-13 in patients with rheumatoid arthritis and its effect on differentiation of Th17 cell subsets in PBMC
作者:
李长超1; 柴艳梅1; 刘 丹2; 周 成1; 陈 蓉1; 黄文峰1
(1.荆门市第二人民医院,湖北 荆门 448000; 2.西安市第五医院,陕西 西安710082 )
Author(s):
LI Changchao; CHAI Yanmei; LIU Dan; ZHOU Cheng; CHEN Rong; HUANG Wenfeng
(Jingmen No.2 People's Hospital,Jingmen 448000,China)
关键词:
类风湿关节炎;  Th17细胞;  白细胞介素-13;  疾病严重程度;  免疫炎性反应;  相关性
Keywords:
Rheumatoid arthritis;  Th17 cell;  Interleukin-13;  Severity of the disease;  Immune inflammatory reaction;  Correlation
分类号:
R 593.2
DOI:
DOI:10.3969/j.issn.1000-7377.2021.02.018
文献标志码:
A
摘要:
目的:探讨类风湿关节炎(RA)患者血清IL-13表达及对RA患者外周血单个核细胞(PBMC)中Th17细胞亚群分化的影响。方法:通过酶联免疫吸附试验(ELISA)检测活动期RA患者(活动组,n=30)、稳定期RA患者(稳定组,n=30)和健康体检者(正常组,n=30)外周血血清白细胞介素-13(IL-13)表达水平,分析血清IL-13水平与RA疾病活动评分(DAS-28)、红细胞沉降率(ESR)及C反应蛋白(CRP)的相关性; 提取RA患者和健康体检者PBMC(设为对照组),分离纯化人CD4+T细胞并用IL-13分组干预,采用ELISA和RT-PCR方法检测其上清和细胞中IL-17A表达水平。结果:①相比正常组,RA患者血清IL-13表达显著升高(P<0.001),其表达水平与DAS28评分、ESR和CRP呈正相关(均P<0.001); ②RA患者CD4+ T细胞经重组人白细胞介素13(rhIL-13)刺激后其上清中IL-17A蛋白及mRNA表达均显著降低(均P<0.001)。结论:IL-13表达水平与RA患者疾病严重程度和免疫炎性反应密切相关,可能通过抑制RA患者CD4+T细胞向Th17分化减少IL-17A分泌而控制RA发展。
Abstract:
Objective:To investigate the expression of serum interleukin(IL)-13 in patients with rheumatoid arthritis and and its effect on differentiation of Th17 cell subsets in peripheral blood mononuclear cell(PBMC).Methods:Enzyme linked immunosorbent assay(ELISA)was used to detect the expression of serum IL-13 in patients with active RA(active group,30 cases),stable RA(stable group,30 cases)and healthy controls(normal group,30 cases).The relationship between serum IL-13 level and disease activity score(DAS28),erythrocyte sedimentation rate(ESR)and C-reactive protein(CRP)was analyzed.PBMCs were isolated from RA patients and healthy people,and human CD4+ T cells were isolated and purified.IL-13 was divided into groups for intervention.The expression level of IL-17A in supernatant and cells was detected by ELISA and RT-PCR.Results:Compared with the normal group,serum IL-13 expression level in RA patients was significantly increased(P<0.001),and its expression level was positively correlated with DAS28 score,ESR and CRP(all P<0.001).The expression of IL-17A protein and mRNA in the supernatant of CD4+T cells in RA patients after stimulation with rhIL-13 was significantly decreased(all P<0.001).Conclusion:The expression level of IL-13 is closely related to the disease severity and immune inflammatory response of RA patients,which may control the development of RA by inhibiting the differentiation of CD4+T cells to Th17 and reducing the secretion of IL-17A.

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备注/Memo

备注/Memo:
基金项目:西安市卫生健康委员会卫生科研人才项目(2020MS06); 西安市科技计划项目20YXYJ0003(7)
更新日期/Last Update: 2021-01-28