[1]肖小娟,魏 星,赵莎彤,等.3-甲基腺嘌呤腹腔注射干预大鼠胃窦组织自噬最佳药物剂量与用药时间探讨[J].陕西医学杂志,2021,50(2):131-134.[doi:DOI:10.3969/j.issn.1000-7377.2021.02.001]
 XIAO Xiaojuan,WEI Xing,ZHAO Shatong,et al.Optimal dose and time of intraperitoneal injection of 3-MA for intervention of gastric antrum autophagy in rats[J].,2021,50(2):131-134.[doi:DOI:10.3969/j.issn.1000-7377.2021.02.001]
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3-甲基腺嘌呤腹腔注射干预大鼠胃窦组织自噬最佳药物剂量与用药时间探讨
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年2期
页码:
131-134
栏目:
基础研究
出版日期:
2021-02-05

文章信息/Info

Title:
Optimal dose and time of intraperitoneal injection of 3-MA for intervention of gastric antrum autophagy in rats
作者:
肖小娟魏 星赵莎彤陈 巧陈丝怡彭 艳
(湖南中医药大学,湖南 长沙 410208)
Author(s):
XIAO XiaojuanWEI XingZHAO ShatongCHEN QiaoCHEN SiyiPENG Yan
(Hunan University of Traditional Chinese Medicine,Changsha 410208,China)
关键词:
3-甲基腺嘌呤 大鼠 腹腔注射 自噬 药物剂量 用药时间
Keywords:
3-Methyladenine Rat Intraperitoneal injection Autophagy Drug dose Medication time
分类号:
R 365
DOI:
DOI:10.3969/j.issn.1000-7377.2021.02.001
文献标志码:
A
摘要:
目的:探讨3-甲基腺嘌呤(3-MA)腹腔注射干预大鼠胃窦组织自噬的最佳药物剂量及用药时间。方法:将21只健康雄性 SD大鼠随机分为七组:A组3-MA 10 mg/kg组、B组3-MA 15 mg/kg组、C组3-MA 30 mg/kg组,D组PBS组,E组空白对照组,F组3-MA 30 mg/kg 3 d组、G组3-MA 30 mg/kg 1 d组,每组3只。3-MA分别按照大鼠体重以剂量10、15、30 mg/kg进行腹腔注射,D组大鼠给予等量PBS溶液腹腔注射,空白对照组不予处理,A、B、C、D、E组干预15 d,F组干预3 d,G组干预1 d。干预结束后取胃窦组织,用Western blot法检测其LC3、P62的表达。结果:LC3Ⅱ/LC3Ⅰ比值大小排序为:D、E组>A组>B组>C组(均P<0.05),G组>C组>F组(均P<0.05); P62相对表达量排序为:C组>B组>A组>D、E组(均P<0.05),C组>G组>F组(均P<0.05)。D、E组LC3Ⅱ/LC3Ⅰ比值和P62相对表达量比较均无统计学差异(均P>0.05)。结论:3-MA腹腔注射可以抑制胃窦组织自噬,其中30 mg/kg为3-MA腹腔注射抑制大鼠胃窦组织自噬的最佳药物剂量,腹腔注射干预15 d自噬抑制效果最明显。
Abstract:
Objective:To explore the optimal dose and time of intraperitoneal injection of 3-MA for intervention of gastric antrum autophagy in rats.Methods:21 healthy male SD rats were randomly divided into 7 groups:A group(3-MA 10 mg/kg),B group(3-MA 15 mg/kg),C group(3-MA 30 mg/kg),D group(PBS),E group(blank control),F group(3-MA 30 mg/kg),G group(3-MA 30 mg/kg),3 rats in each group.3-MA was injected intraperitoneally with doses of 10,15,and 30 mg/kg according to the body weight of rats.The rats in group D were injected intraperitoneally with the same amount of PBS.The blank control group was not treated.A,B,C,D,E groups were intervened for 15 days,F group was intervened for 3 days,and G group was intervened for 1 day.After the intervention,the gastric antrum tissue was taken and the expression of LC3 and p62 was detected by Western blot.Results:The order of LC3Ⅱ/LC3Ⅰ ratio was group D,E > group A > group B > group C(all P<0.05),group G > group C > group F(all P<0.05).The relative expression of P62 was sorted as follows:group C >group B >group A >group D,E(all P<0.05),group C >group G >group F(all P<0.05).There was no significant difference in the ratio of LC3Ⅱ/LC3Ⅰ and the relative expression of P62 between group D and group E(all P>0.05).Conclusion:Intraperitoneal injection of 3-MA can inhibit autophagy of gastric antrum tissue,and 30 mg/kg is the optimal dose in rats,and the optimal intervention time is 15 days.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81774431)
更新日期/Last Update: 2021-01-28