[1]袁 冲,高嘉伟,张玲玲.2017-2019年西安市某三甲医院甲型流行性感冒病毒检测结果与分布特征分析[J].陕西医学杂志,2021,50(1):103-106.[doi:DOI:10.3969/j.issn.1000-7377.2021.01.028]
　YUAN Chong,Gao Jiawei,ZHANG Lingling.Distribution characteristics and detection results of influenza A virus in tertiary grade A hospital in Xi'an,2017-2019[J].,2021,50(1):103-106.[doi:DOI:10.3969/j.issn.1000-7377.2021.01.028]
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2017-2019年西安市某三甲医院甲型流行性感冒病毒检测结果与分布特征分析

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年1期
页码:
103-106
栏目:
调查研究
出版日期:
2021-01-05

文章信息/Info

Title:
Distribution characteristics and detection results of influenza A virus in tertiary grade A hospital in Xi'an,2017-2019
作者:
袁 冲; 高嘉伟; 张玲玲
(空军军医大学西京医院,陕西 西安710032)
Author(s):
YUAN Chong; Gao Jiawei; ZHANG Lingling
(Xijing Hospital,Air Force Medical University,Xi'an 710032,China)
关键词:
甲型流感;  分布特征;  核酸检测;  胶体金法;  病原学
Keywords:
Influenza A virus;  Distribution characteristics;  Nucleic acid detection;  Colloidal gold method;  Etiology
分类号:
R 511.7
DOI:
DOI:10.3969/j.issn.1000-7377.2021.01.028
文献标志码:
A
摘要:
目的:分析西安市某三甲医院流感疑似病例流感病毒检测结果,了解该地区甲型流感流行特征和病原学情况,为流感早期诊治及防控工作提供参考依据。方法: 对2017-2019年就诊于本院的3904例流感疑似病例,采集患者咽拭子标本,用甲型流感抗原检测试剂盒(胶体金法)进行甲型流感病毒快速检测,同时应用Real-time RT-PCR进行流感病毒核酸检测,运用SPSS 19.0软件对检测结果进行统计分析; 然后收集确诊为甲型流感患者的基本信息及病原学监测资料,运用描述性流行病学方法进行分析。结果:共检出甲型流感病毒阳性患者1229例,其中男性679例,检出率为30.93%; 女性550例,检出率为32.18%; 不同性别之间检出率比较差异无统计学意义(χ2=2.74,P >0.05)。甲型流感确诊患者以青、中年为主,分别占39%、33%; 流感高发时间为每年的1、2月及12月,近年来,本院2019年1月确诊患者最多约占48%; 确诊患者分布科室以发热门诊、急诊科、呼吸内科门诊、儿科等居多,依次为31.24%、30.01%、6.51%、4.31%。纳入的3904例流感疑似病例,Real-time RT-PCR法检测病毒RNA阳性率为31.48%(1229/3904),胶体金法检测病毒抗原阳性率为14.16%(553/3904),两组阳性率比较差异有统计学意义(χ2=332.30,P<0.001)。结论: 本地区甲型流感的分布呈现出一些新的特征,需要采取针对性措施,应对甲流患者集中分布科室设置隔离屏障,避免院内交叉感染。胶体金法检测速度快,但对早期感染患者检测效果欠佳,可进一步行甲流病毒核酸检测。
Abstract:
Objective:To analyze the detection results of influenza virus in suspected cases of influenza in tertiary grade A hospital in Xi'an,and to understand the epidemic characteristics and etiology of influenza in this area,so as to provide reference for the early diagnosis,treatment,prevention and control of influenza.Methods:3904 suspected cases of influenza were collected in our hospital from 2017-2019.Rapid detection of influenza A virus was carried out with the influenza A antigen detection kit(colloidal gold method).Influenza A virus nucleic acid was detected with real-time RT-PCR.SPSS 19.0 statistical software package was used for statistical analysis.Then collect the basic information and pathogenic surveillance data of patients diagnosed with influenza A were collected and analyzed by using descriptive epidemiological methods.Results:A total of 1229 cases of influenza A virus were detected,including 679 cases of male(30.9%),550 cases of female(32.8%),and there was no significant difference between the different genders(χ2=2.74,P>0.05).The majority of confirmed influenza A patients were young and middle-aged,accounting for 39% and 33% respectively; the high incidence of influenza A was in January,February and December each year.In recent years,the most confirmed patients in our hospital in January 2019,accounted for about 48%,and the majority of confirmed patients were in fever clinic、emergency department、respiratory department、paediatrics,the proportion was respectively 31.24%,30.01%,6.51%,4.31%.Among the 3904 suspected cases of influenza,the positive rate of PCR method was 31.48%(1229/3904),and the positive rate of colloidal gold method was 14.16%(553/3904),and there was statistically significant difference between the two groups(χ2=332.30,P<0.001).Conclusion:The influenza A virus in this area presents some new characteristics,so it is necessary to take targeted measures to isolate patients and avoid cross-infection.Colloidal gold method has more speed,and its positive results is higher consistency with virus nucleic acid detection,which can be used to diagnose influenza A virus infection,but its false negative rate is also higher.Therefore,when an antigen test is negative but influenza A virus infection is clinically suspected,a further influenza A virus nucleic acid test can be performed.

参考文献/References:

[1] Morimoto K,Hooper DC,Carbaugh H,et al.Rabies virus quasispecies:implications for pathogenesis[J].Proc Natl Aca Sci USA,1998,95(6):3152-3156.
[2] Grebe KM,Yewdell JW,Bennink JR.Heterosubtypic immunity to influenza A vims:where do we stand[J].Microbes and Infection,2008,10:1024-1029.
[3] 张 美,李侗曾,牟丹蕾,等.成人甲型流感病毒核酸持续时间及其相关影响因素分析[J].北京医学,2019,41(6):484-486.
[4] 武涧松,原 杰,贺婷婷,等.流感暴发期间5447例成人流感样病例的流感病毒检测结果与分布特征[J].中华医院感染学杂志,2018,28(23):3521-3526.
[5] 徐翠玲,孙闪华,张彦平,等.2009-2010 年中国内地甲型H1N1流感确诊病例流行特征分析[J].疾病监测,2011,26(10):780-784.
[6] 余云芳,张 谨,马 静,等.2011—2015 年宜昌市流感流行特征及病原学分析[J].公共卫生与预防医学,2016,27(4):38-41.
[7] 刘琳琳,叶国军,方 斌 等.武汉地区普通人群甲型H1N1 流感病毒抗体水平分析[J].现代预防医学,2013,40(3):576-580.
[8] Cauchemez S,Boelle PY,Donnelly CA,et al.Real time estimates in early detection of SARS[J].Emerg Infect Dis,2006,12:110-113.
[9] Fraser C,Donnelly CA,Cauchemez S,et al.Pandemic potential of a strain of influenza A(H1N1):early findings[J].Science,2009,324:1557-1561.
[10] Trifonov V,Khiabanian H,Greenbaum B,et al.The origin o f the recent swine influenza A(H1N1)virus infecting humans[J].Euro Surveill,2009,14(17):191-193.
[11] 龙 芸,王孟昭.流感的流行特征[J].中国临床医学,2001,8(5):580.
[12] 杨厚宇,王旻晋,梁宗安.呼吸道病毒核酸检测结果分析[J].四川医学,2017,38(4):415-420.
[13] 吉茂礼,程 涛.甲型H1N1流感病毒RNA定量分析在轻型与肺炎患者中的应用[J].陕西医学杂志,2018,47(11):1503-1506.

备注/Memo

备注/Memo:
基金项目:陕西省自然基础研究计划项目(S2016YFJQ1066); 中国输血协会威高科研基金资助项目(CSBT-2018-04)
更新日期/Last Update: 2021-01-05