[1]张筱东,谢星星△,张正祥,等.胃癌循环肿瘤细胞膜滤过分离技术检测及其与临床病理特征关系研究[J].陕西医学杂志,2020,49(12):1684-1687.[doi:DOI:10.3969/j.issn.1000-7377.2020.12.039]
　ZHANG Xiaodong,XIE Xingxing,ZHANG Zhengxiang,et al.Detection of circulating tumor cells in gastric cancer by membrane filtration and its correlation with clinicopathological features[J].,2020,49(12):1684-1687.[doi:DOI:10.3969/j.issn.1000-7377.2020.12.039]
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胃癌循环肿瘤细胞膜滤过分离技术检测及其与临床病理特征关系研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年12期
页码:
1684-1687
栏目:
临床病理
出版日期:
2020-12-05

文章信息/Info

Title:
Detection of circulating tumor cells in gastric cancer by membrane filtration and its correlation with clinicopathological features
作者:
张筱东1; 谢星星1△; 张正祥2; 李 佳2; 张 琦2
1.陕西省延安市人民医院检验科(延安 716000); 2.延安大学医学院(延安 716000)
Author(s):
ZHANG Xiaodong; XIE Xingxing; ZHANG Zhengxiang; et al.
Department of Laboratory Medicine,Yan'an People's Hospital,Shaanxi Province(Yan'an 716000)
关键词:
循环肿瘤细胞;  膜滤过分离肿瘤细胞技术;  胃癌;  临床病理特征
Keywords:
Circulating tumor cells;  Isolation by size of epithelial tumor cells;  Gastric cancer;  Clinicopathological characteristics
分类号:
R735.2
DOI:
DOI:10.3969/j.issn.1000-7377.2020.12.039
文献标志码:
A
摘要:
目的:研究胃癌循环肿瘤细胞(CTC)检出情况与胃癌临床病理特征之间的关系。方法:选取胃癌患者84例作为试验组,选取同期30例健康体检者为对照组,采集外周血后用膜滤过分离肿瘤细胞技术(ISET)检测鉴定CTC[包括循环肿瘤微栓(CTM)],并分析其与胃癌患者临床病理特征的关系。结果:对照组外周血标本均未检测到CTC或CTM,试验组外周血标本CTC/CTM总阳性率为 60.71%。不同病理分期胃癌患者CTC/CTM阳性率比较,差异有统计学意义(P<0.05)。不同病理类型胃癌患者外周血 CTC/CTM 阳性率比较,差异没有统计学意义(P>0.05)。胃癌患者外周血 CTC和CTM 检出情况在胃癌瘤块大小、有无淋巴结转移、有无血管侵犯和有无远处转移灶方面比较,差异均有统计学意义(P<0.01)。结论:循环肿瘤细胞检出率与胃癌临床病理特征密切相关,对胃癌的早期诊断和预后判断有潜在的应用价值。
Abstract:
Objective:To study the relationship between the detection of circulating tumor cells(CTC)in gastric cancer and the clinicopathological features of gastric cancer.Methods:84 patients with gastric cancer were selected as the experimental group,and 30 healthy people were selected as the control group.The peripheral blood samples were collected and the isolation by size of epithelial tumor cells(ISET)was used to detect and identify CTC(including CTM).Results:No CTC or CTM was detected in the control group.The total positive rate of CTC and CTM in the experimental group was 60.71%.There was significant difference in the positive rate of CTC and CTM in different pathological stages of gastric cancer(P<0.05).There was no significant difference in the positive rate of CTC and CTM in peripheral blood of patients with different pathological types of gastric cancer(P>0.05).The positive rate of CTC and CTM in peripheral blood of patients with gastric cancer was closely related to tumor size,lymph node metastasis,vascular invasion and distant metastasis(all P<0.01).Conclusion:The detection rate of circulating tumor cells is closely related to the clinicopathological characteristics of gastric cancer,which has potential application value in early diagnosis and prognosis judgment of gastric cancer.

参考文献/References:

[1] Fabisiewicz A,Szostakowska-Rodzos M,Zaczek AJ,et al.Circulating tumor cells in early and advanced breast cancer; biology and prognostic value[J].Int J Mol Sci,2020,21(5):1671.
[2] 蒋晓芬,邵传锋,张 浩,等.循环肿瘤细胞联合癌胚抗原检测在Ⅰ、Ⅱ期结直肠癌诊断中的应用研究[J].中华全科医学,2018,16(10):1653-1655.
[3] Li TT,Liu H,Yu J,et al.Prognostic and predictive blood biomarkers in gastric cancer and the potential application of circulating tumor cells[J].World J gastroenterol,2018,24(21):2236-2246.
[4] Okajima W,Komatsu S,Ichikawa D,et al.Liquid biopsy in patients with hepatocellular carcinoma:circulating tumor cells and cell-free nucleic acids[J].World J Gastroenterol,2017,23(31):5650-5668.
[5] 符兆英.胃癌多药耐药在ABC转运蛋白、细胞凋亡和长链非编码RNA方面的研究进展[J].世界华人消化杂志,2017,25(32):2838-2850.
[6] 米志宽,符兆英,王爱红,等.乳浆大戟抑制原代人胃癌多药耐药细胞增殖和诱导凋亡的研究[J].陕西医学杂志,2017,46(7):836-839.
[7] Fu Z,Han X,Du J,et al.Euphorbia lunulata extract acts on multidrug resistant gastric cancer cells to inhibit cell proliferation,migration and invasion,arrest cell cycle progression,and induce apoptosis[J].J Ethnopharmacol,2018,212:8-17.
[8] Guo X,Han X,Tian Z,et al.Full extract of euphorbia esula reversed chemoresistance,inhibited cell migration/invasion,and induced apoptosis of multidrug-resistant SGC7901/VCR cells[J].Phcog Mag,2018,14(56):411-417.
[9] León-Mateos L,Vieito M,Anido U,et al.Clinical application of circulating tumour cells in prostate cancer:from bench to bedside and back[J].Int J Mol Sci,2016,17(9):1580.
[10] Menyailo ME,Tretyakova MS,Denisov EV.Heterogeneity of circulating tumor cells in breast cancer:identifying metastatic seeds[J].Int J Mol Sci,2020,21(5):1696.
[11] Fu Z,Xiang J.Aptamers,the nucleic acid antibodies,in cancer therapy[J].Int J Mol Sci,2020,21(8):2793.
[12] 田红英,符兆英.SELEX技术的原理和核酸适配体在医学上的应用进展[J].细胞与分子免疫学杂志,2012,28(10):1103-1106.
[13] 韩小东,符兆英,郭姝彤,等.核酸适配体筛选方法研究进展[J].陕西医学杂志,2018,47(3):407-408.
[14] Chen F,Wang S,Fang Y,et al.Feasibility of a novel one-stop ISET device to capture CTCs and its clinical application[J].Oncotarget,2017,8(2):3029-3041.
[15] Sun N,Li X,Wang Z,et al.High-purity capture of CTCs based on micro-beads enhanced isolation by size of epithelial tumor cells(ISET)method[J].Biosens Bioelectron,2018,102:157-163.

更新日期/Last Update: 2020-12-11