[1]高贵洲,张宏瑞,梁晓华,等.竹节参皂苷通过Toll样受体4/核因子-κB信号通路调控免疫功能对肺癌大鼠模型的影响*[J].陕西医学杂志,2020,49(12):1539-1542,1548.[doi:DOI:10.3969/j.issn.1000-7377.2020.12.001]
　GAO Guizhou,ZHANG Hongrui,LIANG Xiaohua,et al.Role of saponins from Panax japonicus inhibiting primary lung cancer through regulating TRL4/NF-κB signaling pathway mediated immunologic function[J].,2020,49(12):1539-1542,1548.[doi:DOI:10.3969/j.issn.1000-7377.2020.12.001]
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竹节参皂苷通过Toll样受体4/核因子-κB信号通路调控免疫功能对肺癌大鼠模型的影响*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年12期
页码:
1539-1542,1548
栏目:
基础研究
出版日期:
2020-12-05

文章信息/Info

Title:
Role of saponins from Panax japonicus inhibiting primary lung cancer through regulating TRL4/NF-κB signaling pathway mediated immunologic function
作者:
高贵洲; 张宏瑞; 梁晓华; 张 涛; 王晓东△
空军军医大学唐都医院胸外科(西安 710038)
Author(s):
GAO Guizhou; ZHANG Hongrui; LIANG Xiaohua; et al.
Department of Thoracic Surgery,Tangdu Hospital,Air Force Medical University(Xi'an 710038)
关键词:
竹节参皂苷;  原发性肺癌;  Toll样受体4;  核因子-κB;  环氧合酶2
Keywords:
Saponins from Panax japonicus;  Primary lung cancer;  TRL4;  NF-κB;  COX2
分类号:
R734.2
DOI:
DOI:10.3969/j.issn.1000-7377.2020.12.001
文献标志码:
A
摘要:
目的:研究竹节参皂苷对原发性肺癌的治疗作用以及对Toll样受体4(TRL4)/核因子-κB(NF-κB)信号通路的调控作用,阐明竹节参皂苷治疗原发性肺癌的作用机制。方法:大鼠灌注碘化油液诱导原发性肺癌模型。造模后1周,竹节参皂苷溶于生理盐水后灌胃给药,连续20周。观察大鼠的一般状态,计算肺癌肿瘤组织的体积和数量,ELISA测定炎症因子表达情况,流式细胞术测定CD3+、CD4+、CD8+免疫细胞数量,Western blot测定TRL4、NF-κB p65和环氧合酶2(COX2)蛋白表达情况。结果:竹节参皂苷可有效抑制肿瘤体积增大和数量增加,减少咳嗽频率,增加CD3+和CD4+免疫细胞数量,减少CD8+数量,抑制血清中炎症因子水平。Western blot结果表明,竹节参皂苷可有效抑制TRL4、NF-κB p65和COX2蛋白表达。结论:竹节参皂苷可有效抑制原发性肿瘤增生,其作用机制可能是通过抑制TRL4/NF-κB信号通路调节机体免疫反应,抑制炎症状态。
Abstract:
Objective:To study the therapeutic effect of saponins from Panax japonicus on primary lung cancer and the regulation of TRL4/NF-κB signaling pathway,and to clarify the mechanism of saponins from Panax japonicus in treating primary lung cancer.Methods:The model of primary lung cancer was induced by infusion of iodized oil in rats.One week after the establishment of the model,saponins from Panax japonicus were dissolved in normal saline and then administered by gavage for 20 consecutive weeks.The general state of rats were observed,and the volumes and quantity of tumor tissue in lung were calculated.The inflammatory factors were determined by ELISA method.Flow cytometry was used to determine the number of CD3+,CD4+ and CD8+ immune cells.The expression of TRL4,NF-κB p65 and COX2 protein was detected by Western blot.Results:Saponins from Panax japonicus could effectively inhibit the increase of tumor volume and quantity,decrease the cough frequency,increase the number of CD3+ and CD4+ immune cells,decrease the number of CD8+ immune cell,and inhibit the level of inflammatory factors in serum.The results of Western blot showed that saponins from Panax japonicus could effectively inhibit the expression of TRL4,NF-κB p65 and COX2 protein.Conclusion:Saponins from Panaxjaponicus can effectively inhibit the proliferation of primary tumor,and its mechanism may be through inhibiting the TRL4/NF-κB signaling pathway to regulate the body's immune response and inhibit inflammation.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目(81672269)
更新日期/Last Update: 2020-12-11