[1]郭 黎,王 静△.核因子-κB信号通路对肺炎模型小鼠免疫功能和炎症反应的影响[J].陕西医学杂志,2020,49(11):1383-1386.[doi:DOI:10.3969/j.issn.1000-7377.2020.11.004]
　GUO Li,WANG Jing..Effect of NF-κB signal pathway on immune function and inflammation level in mice with pneumonia[J].,2020,49(11):1383-1386.[doi:DOI:10.3969/j.issn.1000-7377.2020.11.004]
点击复制

核因子-κB信号通路对肺炎模型小鼠免疫功能和炎症反应的影响
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年11期
页码:
1383-1386
栏目:
基础研究
出版日期:
2020-11-05

文章信息/Info

Title:
Effect of NF-κB signal pathway on immune function and inflammation level in mice with pneumonia
作者:
郭 黎1; 王 静2△
1.陕西省安康市中心医院(安康 725000); 2.陕西省杨凌示范区医院(杨凌 712100)
Author(s):
GUO Li; WANG Jing.
Ankang Central Hospital,Shaanxi Province(Ankang 725000)
关键词:
核因子-κB;  肺炎;  T细胞亚群;  免疫功能;  炎症反应;  二硫代氨基甲酸吡咯烷;  抑制剂
Keywords:
Nuclear factor-kappa B;  Pneumonia;  T cell subsets;  Immune function;  Inflammatory response;  1-Pyrrolidinecarbodithioic acid;  Inhibitor
分类号:
R563.1
DOI:
DOI:10.3969/j.issn.1000-7377.2020.11.004
文献标志码:
A
摘要:
目的:探讨核因子-κB(NF-κB)信号通路对肺炎模型小鼠免疫功能和炎症反应的影响。方法:采用肺炎链球菌诱导肺炎小鼠模型,然后将其分为模型对照组(腹腔注射120 mg/kg生理盐水)和模型+PDTC组(腹腔注射120 mg/kg NF-κB通路抑制剂PDTC),各8只; 另取8只BALB/c小鼠作为正常对照组(腹腔注射等剂量生理盐水),均连续注射2 周后,取各组大鼠右肺上叶组织,测定湿重(W)和干重(D),并计算含水量(TLW)和干湿比(D/W)、Western blot检测各组小鼠肺组织NF-κB蛋白表达、流式细胞术检测各组小鼠外周血中T淋巴细胞亚群CD3+、CD4+、CD8+占比、ELISA试剂盒检测小鼠血清中TNF-α、IL-6和IL-10的含量。结果:①与正常对照组相比,模型对照组肺组织TLW显著增加,而D/W比值显著降低(P<0.05); 与模型对照组相比,模型+PDTC组小鼠肺组织TLW显著降低,D/W比值显著增加(P<0.05)。②与正常对照组相比,模型对照组肺组织NF-κB蛋白相对表达量显著增加(P<0.05); 与模型对照组相比,模型+PDTC组小鼠肺组织NF-κB蛋白相对表达量显著降低(P<0.05)。③与正常对照组相比,模型对照组小鼠外周血CD3+和CD4+百分比以及CD4+/CD8+比值均显著减小(P<0.05); 而与模型对照组相比,模型+PDTC组小鼠以上指标占比或比值均显著升高(P<0.05)。④与正常对照组相比,模型对照组小鼠血清促炎因子TNF-α和IL-6含量均显著升高,抗炎因子IL-10含量显著降低(P<0.05); 而与模型对照组相比,模型+PDTC组小鼠血清中TNF-α和IL-6含量均显著降低,而IL-10含量显著升高(P<0.05)。结论:肺炎的发病机制可能与NF-κB信号通路激活有关,而通过抑制该通路可显著改善肺炎小鼠肺组织病理状态,增强其免疫功能,并改善其炎症反应水平。
Abstract:
Objective:To investigate the effect of nuclear factor-kappaB(NF-κB)signaling pathway on the immune function and inflammation level of pneumonia model mice.Methods:The mice model of pneumonia were induced by Streptococcus pneumonia and divided into model control group(intraperitoneal injection of 120 mg/kg saline)and model + PDTC group(intraperitoneal injection of 120 mg/kg NF-κB pathway inhibitor PDTC),with 8 mice each.Another 8 BALB/c mice were taken as normal control group(intraperitoneal injection of equal dose of saline).After 2 weeks of continuous injection,the upper lobe tissue of the right lung of each group was taken,and the wet weight(W)and dry weight(D)were measured,and the water content(TLW)and dry-wet ratio(D/W)were calculated.Western blot was used to detect the expression of NF-κB protein in the lung tissue of each group.Flow cytometry was used to detect the proportion of T lymphocyte subsets CD3+,CD4+,and CD8+ in the peripheral blood of each group.The contents of TNF-α,IL-6 and IL-10 in the serum of the mice were detected by ELISA kit.Results:Compared with the normal control group,the lung tissue TLW of the model control group increased significantly,while the D/W ratio decreased significantly(P<0.05).Compared with the model control group,the TLW of the lung tissue of the model + PDTC group was significantly reduced,and the D/W ratio was significantly increased(P<0.05).Compared with the normal control group,the relative expression of NF-κB protein in the model control group increased significantly(P<0.05).Compared with the model control group,the relative expression of NF-κB proteinin the model + PDTC group was significantly reduced(P<0.05).Compared with the normal control group,the peripheral blood CD3+ and CD4+ percentages and the CD4+/CD8+ ratio of the model control group were significantly reduced(P<0.05).Compared with the model control group,the proportion or ratio of the above indicators in the model + PDTC group increased significantly(P<0.05).Compared with the normal control group,the serum pro-inflammatory factors TNF-α and IL-6 levels of the model control group were significantly increased,and the anti-inflammatory factor IL-10 level was significantly reduced(P<0.05).Compared with the model control group,the serum levels of TNF-α and IL-6 in the model + PDTC group were significantly reduced,while the IL-10 level was significantly increased(P<0.05).Conclusion:The pathogenesis of pneumonia may be related to the activation of NF-κB signaling pathway.Inhibiting this pathway can significantly improve the pathological state of lung tissue in mice with pneumonia,enhance their immune function,and improve the level of inflammation.

参考文献/References:

[1] 廖 震,楼凌云,管敏昌,等.血清IL-18、IL-33及FeNO与儿童支原体肺炎的相关性研究[J].中华全科医学,2019,17(6):984-986.
[2] 王开金,吴 灿,陆 兵.细菌性肺炎导致感染后咳嗽患者气道炎症及内环境改变的临床研究[J].中国微生态学杂志,2017,29(12):1440-1442.
[3] 刘 菲,高治华.多巴酚丁胺与酚妥拉明联合小剂量多巴胺治疗小儿重症肺炎临床研究[J].陕西医学杂志,2019,48(1):89-91,95.
[4] 钟玉梅.小儿重症肺炎并发症的诊治进展[J].现代医学与健康研究,2017,21(1):193-195.
[5] 杨季云,张思仲,郭 红,等.肿瘤坏死因子α通过激活NF-κB信号通路加快肝细胞周期进程[J].生物化学与生物物理进展,2007,34(6):604-610.
[6] 宋世雄,潘灵辉.调控Rac1/AKT/NF-κB信号通路对呼吸机相关性肺损伤大鼠的影响[J].广西医科大学学报,2018,35(4):454-458.
[7] 张 栋,王庆甫,张晓哲,等.β-catenin与NF-κB信号通路在膝骨关节炎滑膜炎症中的表达特点及意义[J].中国骨伤,2019,32(8):721-726.
[8] 张辉果,董志巧,王晓利,等.麻杏石甘汤及其拆方对肺炎链球菌致肺炎小鼠的影响[J].中成药,2019,41(9):2084-2089.
[9] 王 佳,冯星火,国丽娜,等.银翘散对流感病毒感染性重症肺炎小鼠肺组织的修复机制研究[J].陕西中医,2020,41(2):156-159.
[10] 刘 霞.超声雾化吸入治疗小儿肺炎的疗效观察及护理[J].临床医学研究与实践,2017,2(24):192-193.
[11] 赵丽秦,武一鸣.快速血清学和微生物培养检测对小儿肺炎支原体感染的诊断价值[J].临床医学研究与实践,2018,3(5):121-122.
[12] Sinan E,Celia P,Bernard H.Diagnosis and management of community and hospital acquired pneumonia in adults:Delayed prescription of antibiotics is nothing more than a rearguard action[J].BMJ,2017,349:6722.
[13] Ai JY,Kun XI,Yan QY,et al.Effect of Qingfei Mixture on pediatric mycoplasma pneumoniae pneumonia with phlegm heat obstructing fei(lung)syndrome[J].Chinese Journal of Combined Medicine:English Edition,2017,23(9):681-688.
[14] 邓云森,李盛强,林丽妮,等.血清25-(OH)D、hs-CRP、PCT与新生儿重症肺炎的关系研究[J].中国急救复苏与灾害医学杂志,2020,15(1):75-77.
[15] 陈灵红,杨德华,林红霞.儿童重症肺炎支原体肺炎的细胞因子水平观察[J].全科医学临床与教育,2017,15(4):432-435.

相似文献/References:

[1]金 璞,任郭侠△,赵 琳.陕西省新型冠状病毒肺炎流行病学与时空分布情况分析[J].陕西医学杂志,2020,49(5):515.
　JIN Pu,REN Guoxia,ZHAO Lin..Epidemiology and spatiotemporal distribution of COVID-19 in Shaanxi Province[J].,2020,49(11):515.
[2]杨建华,马伟欢△,宁 博,等.陕西宝鸡市新型冠状病毒肺炎流行病学及临床特征分析[J].陕西医学杂志,2020,49(5):519.
　YANG Jianhua,MA Weihuan,Ning Bo,et al.Epidemiological and clinical characteristics of NCP patients in Baoji,Shaanxi[J].,2020,49(11):519.
[3]胡 伟.布地奈德雾化吸入治疗新生儿肺炎临床效果及对患儿各项潮气呼吸指标的影响研究[J].陕西医学杂志,2021,50(3):353.[doi:DOI:10.3969/j.issn.1000-7377.2021.03.025]
　HU Wei.Therapeutic effect of budesonide aerosol inhalation in treatment of neonatal pneumonia and its influence on tidal breathing indexes and adverse reactions[J].,2021,50(11):353.[doi:DOI:10.3969/j.issn.1000-7377.2021.03.025]
[4]顾盼瑾,张立红,胡锡池,等.黄芪多糖联合替加环素对白细胞减少性鲍曼不动杆菌感染肺炎大鼠的疗效及机制研究[J].陕西医学杂志,2023,52(10):1319.[doi:DOI:10.3969/j.issn.1000-7377.2023.10.008]
　GU Panjin,ZHANG Lihong,HU Xichi,et al.Effect and mechanism analysis of astragalus polysaccharide combined with tigacycline in rats infected with pneumonia caused by leukopenic acinetobacter baumannii[J].,2023,52(11):1319.[doi:DOI:10.3969/j.issn.1000-7377.2023.10.008]

更新日期/Last Update: 2020-11-05