[1]常 江,张晓乐,余 华,等.绞股蓝总皂苷预处理对大鼠蛛网膜下腔出血后脑水肿和神经炎症反应的影响*[J].陕西医学杂志,2020,49(11):1379-1382,1386.[doi:DOI:10.3969/j.issn.1000-7377.2020.11.003]
　CHANG Jiang,ZHANG Xiaole,YU Hua,et al.Effect of gypenosides on cerebral edema and neuroinflammation after subarachnoid hemorrhage in rats[J].,2020,49(11):1379-1382,1386.[doi:DOI:10.3969/j.issn.1000-7377.2020.11.003]
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绞股蓝总皂苷预处理对大鼠蛛网膜下腔出血后脑水肿和神经炎症反应的影响*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年11期
页码:
1379-1382,1386
栏目:
基础研究
出版日期:
2020-11-05

文章信息/Info

Title:
Effect of gypenosides on cerebral edema and neuroinflammation after subarachnoid hemorrhage in rats
作者:
常 江1; 张晓乐1; 余 华1; 陈 杰1; 李俊玲2△
1.陕西省中医医院脑病科(西安710003); 2.西安市中医医院妇科(西安710021)
Author(s):
CHANG Jiang; ZHANG Xiaole; YU Hua; et al.
Department of Brain Diseases,Shaanxi Provincial Hospital of Traditional Chinese Medicine(Xi'an 710003)
关键词:
绞股蓝总皂苷;  蛛网膜下腔出血;  脑水肿;  神经炎症;  细胞凋亡
Keywords:
Gypenosides;  Subarachnoid hemorrhage;  Cerebral edema;  Neuronflammation;  Apoptosis
分类号:
R743.3
DOI:
DOI:10.3969/j.issn.1000-7377.2020.11.003
文献标志码:
A
摘要:
目的:研究绞股蓝总皂苷预处理对大鼠蛛网膜下腔出血后脑水肿和神经炎症反应的影响。方法:雄性SD大鼠96只,体重为235～260 g,分为四组:假手术对照组(Sham组)、蛛网膜下腔出血组(SAH组)、绞股蓝总皂苷200 mg/(kg·d)组(GP1组)、绞股蓝总皂苷400 mg/(kg·d)组(GP2组)。Sham组线头不刺破血管壁; SAH组大鼠建立SAH模型; GP1和GP2组每天口饲一次绞股蓝总皂苷溶液(溶于无菌生理盐水)200或400 mg/kg共7 d,之后建立SAH模型。模型建立后行脑水含量检测和Evans blue染色,采用ELISA法检测肿瘤坏死因子-α(TNF-α)、白细胞介素6(IL-6)和白细胞介素1β(IL-1β)含量; 采用TUNEL染色法检测脑皮层凋亡细胞数; 采用Western blot法检测脑Nrf2蛋白表达水平。结果:SAH组大鼠脑水含量和外渗率较Sham组显著增多(P<0.05),脑组织促炎因子TNF-α、IL-6、IL-1β表达增加(P<0.05),TUNEL阳性凋亡细胞明显增多(P<0.05),脑Nrf2蛋白表达表达显著下降(P<0.05); GP1和GP2组大鼠较SAH组脑水含量和Evans blue外渗率均明显减少(P<0.05),TNF-α、IL-6、IL-1β含量下调(P<0.05),TUNEL阳性凋亡细胞明显减少(P<0.05),脑Nrf2蛋白明显增加(P<0.05)。结论:绞股蓝总皂苷预处理可通过激活Nrf2信号通路减轻蛛网膜下腔出血造成的脑损伤和神经炎症反应。
Abstract:
Objective:To study the effect of gypenosides on cerebral edema and neuroinflammation after subarachnoid hemorrhage in rats.Methods:A total of 96 male SD rats weighing 235-260 g were randomly arranged into 4 groups:sham-operated control group(Sham group),subarachnoid hemorrhage group(SAH group),gypenosides 200 mg/(kg·d)group(GP1 group),gypenosides 400 mg/(kg·d)group(GP2 group).The endovascular perforation model of SAH was used in the present study.In the Sham group,the tip of nylon filament was merely sent to the right internal carotid artery but without puncture of the artery wall.The SAH model was established in the SAH group.The gypenosides solution was orally administered daily in the GP1 group [200 mg(kg·d)] and the GP2 group [400 mg(kg·d)] respectively for 7 days,followed by establishment of the SAH model.The content of cerebral water and the extravasation of Evans blue were detected 24 h after the surgery to evaluate the extent of brain edema and blood-brain barrier disruption.The protein concentration of TNF-α,IL-6 and IL-1β was examined by ELISA.TUNEL staining was used to evaluate the apoptosis in the injured cortex.The protein expression of Nrf2 was detected by Western blot.Results:Compared with the Sham group,the content of cerebral water and the extravasation of Evans blue in the SAH group were significantly increased(P<0.05),the levels of TNF-α,IL-6 and IL-1β were up-regulated(P<0.05),TUNEL positive apoptotic cells were markedly increased(P<0.05),and the protein expression of Nrf2 was significantly down-regulated(P<0.05).Compared with the SAH group,the content of cerebral water and the extravasation of Evans blue in the rats of GP1 and GP2 group were diminished(P<0.05),the levels of TNF-α,IL-6 and IL-1β were reduced(P<0.05),TUNEL positive apoptotic cells were substantially decreased(P<0.05),and the protein expression of Nrf2 in the brain was up-regulated(P<0.05).Conclusion:The pretreatment of gypenosides can relieve cerebral edema and blood-brain barrier destruction,reduce neuroinflammation,and attenuate apoptosis after subarachnoid hemorrhage.This phenomenon may be associated with activation of Nrf2 signaling pathway.

参考文献/References:

[1] Lantigua H,Ortega-Gutierrez S,Schmidt JM,et al.Subarachnoid hemorrhage:who dies,and why[J].Crit Care,2015,19:309.
[2] Juvela S,Korja M.Intracranial aneurysm parameters for predicting a future subarachnoid hemorrhage:a long-term follow-up study[J].Neurosurgery,2017,81(3):432-440.
[3] 刘永军,高 翔,刘存祥,等.脑池积血对动脉瘤性蛛网膜下腔出血病人术后急性脑积水的影响[J].中西医结合心脑血管病杂志,2018,16(2):227-229.
[4] Ahn SH,Savarraj JP,Pervez M,et al.The subarachnoid hemorrhage early brain edema score predicts delayed cerebral ischemia and clinical outcomes[J].Neurosurgery,2018,83(1):137-145.
[5] Zhang T,Su J,Guo B,et al.Apigenin protects blood-brain barrier and ameliorates early brain injury by inhibiting TLR4-mediated inflammatory pathway in subarachnoid hemorrhage rats[J].Int Immunopharmacol,2015,28(1):79-87.
[6] Wang CX,Xie GB,Zhou CH,et al.Baincalein alleviates early brain injury after experimental subarachnoid hemorrhage in rats:possible involvement of TLR4/NF-kappaB-mediated inflammatory pathway[J].Brain Res,2015,1594:245-255.
[7] 杨 彬.右美托咪定预处理对实验性蛛网膜下腔出血大鼠的脑保护作用及机制[J].中国老年学杂志,2019,39(19):4829-4831.
[8] Mu RH,Fang XY,Wang SS,et al.Antidepressant-like effects of standardized gypenosides:involvement of brain-derived neurotrophic factor signaling in hippocampus[J].Psychopharmacology(Berl),2016,233(17):3211-3221.
[9] 赵 涛,乐 静,李传静,等.绞股蓝总皂苷对2型糖尿病大鼠游离脂肪酸代谢的影响及改善胰岛素抵抗相关机制研究[J].安徽医药,2017,23(1):42-46.
[10] Lee B,Shim I,Lee H.Gypenosides attenuate lipopolysaccharide-induced neuroinflammation and memory impairment in rats[J].Evid Based Complement Alternat Med,2018,2018:4183670.
[11] 阮 丹,雷 婧.绞股蓝总皂苷对非酒精性脂肪肝大鼠Treg/Th17免疫功能的影响[J].中国现代应用药学,2017(12):1683-1688.
[12] Wang XJ,Sun T,Kong L,et al.Gypenosides pre-treatment protects the brain against cerebral ischemia and increases neural stem cells/progenitors in the subventricular zone[J].Int J Dev Neurosci,2014,33:49-56.
[13] Guo Z,Hu Q,Xu L,et al.Lipoxin A4 reduces inflammation through formyl peptide receptor 2/p38 mapk signaling pathway in subarachnoid hemorrhage rats[J].Stroke,2016,47(2):490-497.
[14] Bederson JB,Germano IM,Guarino L.Cortical blood flow and cerebral perfusion pressure in a new noncraniotomy model of subarachnoid hemorrhage in the rat[J].Stroke,1995,26(6):1086-1091.
[15] Wang XJ,Sun T,Kong L,et al.Gypenosides pre-treatment protects the brain against cerebral ischemia and increases neural stem cells/progenitors in the subventricular zone[J].Int J Dev Neurosci,2014,33:49-56.
[16] Altay O,Suzuki H,Hasegawa Y,et al.Isoflurane on brain inflammation[J].Neurobiol Dis,2014,62:365-371.
[17] Savarraj J,Parsha K,Hergenroeder G,et al.Early brain injury associated with systemic inflammation after subarachnoid hemorrhage[J].Neurocrit Care,2018,28(2):203-211.
[18] 邹长林,李明昌.炎症在蛛网膜下腔出血中的作用及机制研究进展[J].国际神经病学神经外科学杂志,2017,44(1):83-86.
[19] Wang P,Niu L,Guo X D,et al.Gypenosides protects dopaminergic neurons in primary culture against MPP(+)-induced oxidative injury[J].Brain Res Bull,2010,83(5):266-271.
[20] Qi G,Zhang L,Xie WL,et al.Protective effect of gypenosides on DNA and RNA of rat neurons in cerebral ischemia-reperfusion injury[J].Acta Pharmacol Sin,2000,21(12):1193-1196.
[21] 常 江,余 华,张晓乐,等.毛蕊异黄酮对大鼠蛛网膜下腔出血后血脑屏障和氧化应激反应的影响[J].吉林中医药,2019,39(10):1347-1350.
[22] Ahmed SM,Luo L,Namani A,et al.Nrf2 signaling pathway:pivotal roles in inflammation[J].Biochim Biophys Acta,2017,863(2):585-597.

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备注/Memo

备注/Memo:
*陕西省科技统筹创新工程计划项目(2015KTZDSF02-03)
更新日期/Last Update: 2020-11-05