[1]信 波,张开亮△,宋晓鹏,等.雌激素受体β拮抗剂对非小细胞肺癌A549细胞增殖、迁移及侵袭能力的影响*[J].陕西医学杂志,2020,49(10):1223-1227.[doi:DOI:10.3969/j.issn.1000-7377.2020.10.006]
　XIN Bo,ZHANG Kailiang,SONG Xiaopeng,et al.Effect of estrogen receptor β antagonist PHTPP on proliferation,migration and invasion of non-small cell lung cancer A549 cells[J].,2020,49(10):1223-1227.[doi:DOI:10.3969/j.issn.1000-7377.2020.10.006]
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雌激素受体β拮抗剂对非小细胞肺癌A549细胞增殖、迁移及侵袭能力的影响*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年10期
页码:
1223-1227
栏目:
基础研究
出版日期:
2020-10-05

文章信息/Info

Title:
Effect of estrogen receptor β antagonist PHTPP on proliferation,migration and invasion of non-small cell lung cancer A549 cells
作者:
信 波1; 张开亮1△; 宋晓鹏1; 李海波1; 贾 丽1; 庞海林2
1.解放军第960医院泰安医疗区(泰安 271000); 2.空军军医大学唐都医院肿瘤科(西安 710038)
Author(s):
XIN Bo; ZHANG Kailiang; SONG Xiaopeng; et al.
Tai'an Medical District,the 960th Hospital of PLA(Tai'an 271000)
关键词:
雌激素受体β拮抗剂;  非小细胞肺癌;  增殖;  迁移;  侵袭
Keywords:
Estrogen receptor β;  Non-small cell lung cancer;  Proliferation;  Migration;  Invasion
分类号:
R734.2
DOI:
DOI:10.3969/j.issn.1000-7377.2020.10.006
文献标志码:
A
摘要:
目的:观察雌激素受体β拮抗剂(PHTPP)对非小细胞肺癌(NSCLC)A549细胞增殖、迁移和侵袭的影响。方法:利用MTT法筛选出PHTPP对A549细胞增殖产生作用的有效浓度和时间; 利用平板克隆形成实验检测PHTPP对A549细胞增殖能力的影响; 利用Transwell实验分别检测PHTPP对A549细胞迁移和侵袭能力的影响。结果:平板克隆实验结果显示PHTPP可拮抗雌激素受体β激动剂(DPN)对A549细胞增殖能力的提高(P<0.05)。Transwell实验结果显示PHTPP可拮抗DPN对A549细胞迁移和侵袭能力的提高(P<0.05)。结论:在体外,应用PHTPP可拮抗在激动雌激素受体β时,A549细胞所提高的增殖、迁移和侵袭能力。
Abstract:
Objective:To observe the effect of estrogen receptor β antagonist(PHTPP)on the proliferation,migration and invasion of non-small cell lung cancer(non-small cell lung cancer,NSCLC)A549 cells.Methods:MTT method was used to screen the effective concentration and time of PHTPP on A549 cell proliferation,plate clone formation assay was used to detect the effect of PHTPP on A549 cell proliferation,and Transwell assay was used to detect the effect of PHTPP on A549 cell migration and invasion.Results:The results of plate cloning assay showed that PHTPP could antagonize the effect of estrogen receptor β agonist(DPN)on the proliferation of A549 cells(P<0.05).The results of Transwell assay showed that PHTPP could antagonize the improvement of migration and invasion of A549 cells induced by DPN(P<0.05).Conclusion:In vitro,PHTPP could antagonize the increased proliferation,migration and invasion of A549 cells when estrogen receptor β was activated.

参考文献/References:

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目(81272348,81902318)
更新日期/Last Update: 2020-10-10