[1]王松海,许 鹏,康 超,等.红景天苷对MPTP-帕金森病小鼠模型自噬通路影响的实验研究[J].陕西医学杂志,2020,49(10):1215-1218.[doi:DOI:10.3969/j.issn.1000-7377.2020.10.004]
　WANG Songhai,XU Peng,KANG Chao,et al.Effect of salidroside on the autophagy pathway in the MPTP-induced mouse model of Parkinson's disease[J].,2020,49(10):1215-1218.[doi:DOI:10.3969/j.issn.1000-7377.2020.10.004]
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红景天苷对MPTP-帕金森病小鼠模型自噬通路影响的实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年10期
页码:
1215-1218
栏目:
基础研究
出版日期:
2020-10-05

文章信息/Info

Title:
Effect of salidroside on the autophagy pathway in the MPTP-induced mouse model of Parkinson's disease
作者:
王松海; 许 鹏; 康 超; 陈 捷; 杨晨光; 谢燕华△
陕西省中医医院肿瘤科(西安 710003)
Author(s):
WANG Songhai; XU Peng; KANG Chao; et al.
Department of Oncology,Shaanxi Hospital of Traditional Chinese Medicine(Xi'an 710003)
关键词:
红景天苷;  帕金森病;  小鼠黑质纹状体;  自噬通路相关蛋白
Keywords:
Salidroside;  Parkinson's disease;  Mouse substantia nigra striatum;  Autophagy pathway related proteins
分类号:
R742
DOI:
DOI:10.3969/j.issn.1000-7377.2020.10.004
文献标志码:
A
摘要:
目的:探讨红景天苷(Sal)对帕金森病(PD)小鼠黑质纹状体(SNpc)自噬通路相关蛋白表达的影响及其可能机制。方法:爬杆实验检测小鼠的行为学变化; 免疫荧光组织化学法检测黑质纹状体TH阳性神经元数目的变化; Western blot 检测自噬相关蛋白的表达情况。结果:模型组小鼠Tturn及TLA时间明显延长(P<0.01),与模型组比较,治疗组Tturn及TLA时间明显缩短(P<0.05); 模型组黑质纹状体中TH 阳性神经元的数目明显减少(P<0.01),Sal 干预后小鼠TH阳性神经元有所恢复(P<0.05); 1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)处理后黑质纹状体中LC-3β、Beclin1表达增多,PINK1、Parkin表达减少,而Sal预处理可以明显改善这些变化(P<0.05)。结论:Sal 对 PD小鼠DA能神经元具有保护作用,而其机制可能与抑制黑质纹状体中自噬通路相关蛋白的表达有关。
Abstract:
Objective:To investigate the effect of salidroside(Sal)on the expression of autophagy pathway related proteins in substantia nigra pars compacta(SNpc)of Parkinson's disease(PD)mice and its possible mechanism.Methods:The behavioral changes of the mice were detected by the pole test.The number of TH positive neurons in the SNpc was detected by immunofluorescence histochemistry.The expression of autophagy related proteins was detected by Western blot.Results:Compared with the control group,the model group had significantly longer T-turn and T-LA(P<0.01),while the Sal group had shorter time(P<0.05).The number of TH positive neurons in the SNpc of model group was significantly less than that of control group(P<0.01).After Sal intervention,the number of TH positive neurons recovered(P<0.05).The expression of LC-3β and Beclin 1 increased,PINK1 and Parkin decreased in the SNpc of MPTP-group.However,Sal group could significantly inhibit these changes(P<0.05).Conclusion:Salidroside has protective effect on DA neurons of PD mice,and the mechanism may be related to the inhibition of autophagy pathway related proteins in SNpc.

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更新日期/Last Update: 2020-10-10