[1]张树鹰,冯 瑶.沉默SOCS1基因表达对人口腔癌KB细胞增殖的机制研究*[J].陕西医学杂志,2020,49(10):1211-1214.[doi:DOI:10.3969/j.issn.1000-7377.2020.10.003]
　ZHANG Shuying,FENG Yao..Mechanism of silencing SOCS1 gene table on proliferation of human oral cancer KB cells[J].,2020,49(10):1211-1214.[doi:DOI:10.3969/j.issn.1000-7377.2020.10.003]
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沉默SOCS1基因表达对人口腔癌KB细胞增殖的机制研究*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年10期
页码:
1211-1214
栏目:
基础研究
出版日期:
2020-10-05

文章信息/Info

Title:
Mechanism of silencing SOCS1 gene table on proliferation of human oral cancer KB cells
作者:
张树鹰1; 冯 瑶2
1.锦州医科大学附属第二医院口腔惠民综合门诊(锦州 121000); 2.佳木斯大学附属口腔医院牙体牙髓一科(佳木斯154004)
Author(s):
ZHANG Shuying; FENG Yao.
The Comprehensive Outpatient Department of Stomatology for the Benefit of the People, the Second Affiliated Hospital of Jinzhou Medical University(Jinzhou 121000)
关键词:
口腔癌;  小干扰RNA;  细胞因子信号转导抑制因子1基因;  增殖;  凋亡;  侵袭
Keywords:
Oral cancer;  siRNA;  SOCS1 gene;  Proliferation;  Apoptosis;  Invasion
分类号:
R739.8
DOI:
DOI:10.3969/j.issn.1000-7377.2020.10.003
文献标志码:
A
摘要:
目的:探究小干扰RNA(siRNA)沉默细胞因子信号转导抑制因子1(SOCS1)基因对人口腔癌KB细胞增殖、凋亡及侵袭的影响,旨在为口腔癌患者基因靶向治疗提供参考。方法:将培养好的人口腔癌KB细胞随机分为空白对照组、阴性组、转染组,siRNA沉默SOCSl基因、阴性对照质粒分别转染至转染组、阴性组,而空白对照组不作任何处理,SOCS1基因沉默后,四唑盐法检测三组KB细胞增殖率、流式细胞术检测各组KB细胞凋亡率,同时检测三组细胞侵袭能力。结果:siRNA沉默SOCS1基因在人口腔癌KB细胞中呈阳性表达(细胞膜或细胞质呈棕黄色),转染组基因沉默后24、48、72、96 h人口腔癌KB细胞增殖率明显低于阴性组,比较差异有统计学意义(P<0.05); 转染组人口腔癌KB细胞凋亡率高于空白对照组和阴性组,空白对照组人口腔癌KB细胞凋亡率高于阴性组,比较差异有统计学意义(P<0.05); 转染组穿膜细胞数低于空白对照组和阴性组,阴性组穿膜细胞数低于空白对照组,差异有统计学意义(P<0.05)。结论:siRNA沉默SOCS1基因可减弱人口腔癌KB细胞的增殖能力,并抑制其侵袭能力,有望为口腔癌患者靶向抗肿瘤药物的研发提供新思路。
Abstract:
Objective:To investigate the effect of silencing suppressor of cytokine signal transduction 1(SOCS1)gene by small interfering RNA(siRNA)on the proliferation,apoptosis and invasion of human oral canxcer KB cells,and to provide reference for gene-targeted therapy of patients with oral cancer.Methods:The cultured human oral cancer KB cells were divided into blank control group,negative group and transfection group.The siRNA silencing SOCS1 gene and negative control plasmid were transfected into the transfection group and the negative group,but the blank control group was not given any treatment.After SOCS1 gene silencing,the proliferation rates and the apoptosis rates of KB cells were detected by methyl thiazolyl tetrazolium method and flow cytometry,respectively.The invasion of KB cells was also detected.Results:There was positive expression of siRNA silencing SOCS1 gene in human oral cancer KB cells(brown-yellow cell membrane or cytoplasm).The proliferation rate of human oral cancer KB cells in the transfection group were significantly lower than the negative group at 24 h,48 h,72 h and 96 h after gene silencing(P<0.05).The apoptosis rate of KB cells in the transfection group was higher than that in the blank control group and the negative group,and that in the blank control group was higher than that in the negative group(P<0.05).The number of cells permeating the membrane in the transfection group was smaller than that in the blank control group and the negative group,and that in the negative group was smaller than that in the blank control group(P<0.05).Conclusion:SiRNA silencing SOCS1 gene can weaken the proliferation and inhibit invasion of human oral cancer KB cells.It may provide new ideas for development of targeted anti-tumor drugs for oral cancer patients.

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备注/Memo

备注/Memo:
*黑龙江省卫生和计划生育委员会科研课题(2017-417)
更新日期/Last Update: 2020-10-10