[1]张送萍,尹凌凡,毕艳慧.宫颈高危型人乳头瘤病毒感染与阴道菌群及血清氧化应激水平关系探讨[J].陕西医学杂志,2020,49(8):975.[doi:DOI:10.3969/j.issn.1000-7377.2020.08.016]
　ZHANG Songping,YIN Lingfan,BI Yanhui..Relationship between cervical HR-HPV infection and vaginal flora and serum oxidative stress level[J].,2020,49(8):975.[doi:DOI:10.3969/j.issn.1000-7377.2020.08.016]
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宫颈高危型人乳头瘤病毒感染与阴道菌群及血清氧化应激水平关系探讨
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年8期
页码:
975
栏目:
临床研究
出版日期:
2020-08-05

文章信息/Info

Title:
Relationship between cervical HR-HPV infection and vaginal flora and serum oxidative stress level
作者:
张送萍; 尹凌凡; 毕艳慧
北京中医药大学深圳医院(深圳518172)
Author(s):
ZHANG Songping; YIN Lingfan; BI Yanhui.
Shenzhen Hospital of Beijing University of Chinese Medicine(Shenzhen 518172)
关键词:
人乳头瘤病毒;  阴道菌群;  氧化应激;  淋巴细胞;  炎性因子;  免疫
Keywords:
Human papillomavirus;  Vaginal flora;  Oxidative stress;  Lymphocytes;  Inflammatory factors;  Immune
分类号:
R711.31
DOI:
DOI:10.3969/j.issn.1000-7377.2020.08.016
文献标志码:
A
摘要:
目的:探讨宫颈高危型人乳头瘤病毒(HR-HPV)感染与阴道菌群及血清氧化应激水平的关系。方法:选取有生殖道感染症状的患者112例作为研究对象,采集宫颈分泌物检测HR-HPV感染情况、CD4+T与CD8+T淋巴细胞比例,检测宫颈阴道中病原菌分布情况,抽取外周血检测血清氧化应激水平及炎性因子浓度,分析HR-HPV感染与阴道菌群、局部免疫微环境及血清氧化应激状态的关系。结果:经宫颈分泌物检查结果显示,112例患者HR-HPV感染阳性患者47例(占41.96%); HR-HPV阳性组衣原体、解脲脲原体与细菌性阴道炎检出率明显高于HR-HPV阴性组(P<0.05); HR-HPV阳性组宫颈分泌物CD4+T淋巴细胞比例与CD4+T/CD8+T比值明显低于HR-HPV阴性组(P<0.05)。HR-HPV阳性组血清总氧化态(TOS)、氧化应激指数(OSI)、γ-干扰素(IFN-γ)与白介素2(IL-2)浓度明显高于HR-HPV阴性组(P<0.05),而HR-HPV阳性组血清总还原态(TAS)与白介素4(IL-4)浓度明显低于HR-HPV阴性组(P<0.05)。多因素Logisic回归分析结果显示,HR-HPV感染与衣原体、解脲脲原体感染率呈现独立正相关关系(P<0.05),与血清TOS、OSI、IFN-γ及IL-2浓度呈现独立正相关关系(P<0.05),与宫颈分泌物中CD4+T/CD8+T比值及血清TAS呈现负相关关系(P<0.05)。结论:宫颈HR-HPV感染患者阴道衣原体与解脲脲原体感染率升高,感染部位CD4+T/CD8+T比值降低,血清呈现高炎性因子水平与氧化应激状态。
Abstract:
Objective:To investigate the relationship between cervical high-risk human papillomavirus(HR-HPV)infection and vaginal flora and serum oxidative stress level.Methods:112 patients with reproductive tract infection were selected as the study objects.Cervical secretion was collected to detect the infection of HR-HPV,the proportion of CD4+T and CD8+T lymphocytes,the distribution of pathogenic bacteria in cervix and vagina.The level of serum oxidative stress and the concentration of inflammatory factors were detected in peripheral blood.The relationship between HR-HPV infection and vaginal flora,local immune microenvironment and serum oxidative stress was analyzed.Results:The results of cervical secretion examination showed that 47 of 112 patients were positive for HR-HPV infection,accounting for 41.96%; the detection rate of Chlamydia,Ureaplasma urealyticum and bacterial vaginitis in HR-HPV positive group was significantly higher than that in HR-HPV negative group(P<0.05); the ratio of CD4+T lymphocyte and CD4+T/CD8+T in HR-HPV positive group was significantly lower than that in HR-HPV negative group(P<0.05).The concentrations of total oxidation state(TOS),oxidative stress index(OSI),interferon gamma(IFN-γ)and interleukin-2(IL-2)in HR-HPV positive group were significantly higher than those in HR-HPV negative group(P<0.05),while the concentrations of total reduction state(TAS)and interleukin-4(IL-4)were significantly lower than those in the HR-HPV negative group(P<0.05).Multivariate Logistic regression analysis showed that HR-HPV infection was positively correlated with Chlamydia and Ureaplasma urealyticum infection rate(P<0.05),and positively correlated with the concentrations of serum TOS,OSI,IFN-γ and IL-2(P<0.05),and negatively correlated with CD4+T/CD8+T ratio and serum TAS in cervical secretion(P<0.05).Conclusion:The infection rate of Chlamydia and Ureaplasma urealyticum in the vagina of patients with cervical HR-HPV infection is increased,the ratio of CD4+T/CD8+T in the infected site is decreased,the high levels of inflammatory factors and oxidative stress state in serum are closely related to HR-HPV infection.

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更新日期/Last Update: 2020-08-04