[1]孟卫荣,李珍珍,韩军涛△.RORα在脂多糖所致小鼠巨噬细胞炎症反应中的表达变化与作用实验研究*[J].陕西医学杂志,2020,49(7):788-791.[doi:DOI:10.3969/j.issn.10007377.2020.07.005]
 MENG Weirong,LI Zhenzhen,HAN Juntao..Expression and role of RORα in lipopolysaccharide induced macrophage inflammatory response in mice[J].,2020,49(7):788-791.[doi:DOI:10.3969/j.issn.10007377.2020.07.005]
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RORα在脂多糖所致小鼠巨噬细胞炎症反应中的表达变化与作用实验研究*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年7期
页码:
788-791
栏目:
基础研究
出版日期:
2020-07-05

文章信息/Info

Title:
Expression and role of RORα in lipopolysaccharide induced macrophage inflammatory response in mice
作者:
孟卫荣李珍珍韩军涛
空军军医大学第一附属医院烧伤与皮肤外科(西安 710032)
Author(s):
MENG WeirongLI ZhenzhenHAN Juntao.
Department of Burns and Cutaneous Surgery,the First Affiliated Hospital of Air Force Medical University(Xi'an 710032)
关键词:
RORα 巨噬细胞 脂多糖 炎症反应 小鼠 炎症因子
Keywords:
RORα Macrophage Lipopolysaccharide Inflammatory reaction Mouse Inflammatory factor
分类号:
R364.5
DOI:
DOI:10.3969/j.issn.10007377.2020.07.005
文献标志码:
A
摘要:
目的:探讨RORα在脂多糖所致小鼠巨噬细胞炎症反应过程中的表达变化与作用。方法:取小鼠骨髓来源的巨噬细胞(BMDMs),加入100 ng/ml脂多糖(LPS)处理不同时间点后,利用荧光定量PCR(qRT-PCR)法检测细胞中炎症因子白介素1β(IL-1β)和肿瘤坏死因子-α(TNF-α),以及RORα的转录水平变化,进一步利用Western blot检测RORα蛋白水平变化; 分别采用RORα激动剂SR1078和拮抗剂SR3335预处理细胞24 h后,LPS处理2 h,利用ELISA法检测细胞上清中炎症因子IL-1β,TNF-α和IL-6的含量。结果:100 ng/ml LPS在不同时间点均可显著促进BMDMs炎症因子IL-1β和TNF-α的转录水平表达(P<0.05),而在LPS处理1~12 h显著抑制了RORα的转录水平(P<0.05),同时蛋白表达水平也明显降低(P<0.05)。与LPS处理组相比,SR1078预处理,可显著抑制炎症因子的释放,而SR3335进一步激活了细胞炎症因子的释放(P<0.05)。结论:RORα对脂多糖所致小鼠巨噬细胞炎症反应具有调节作用。
Abstract:
Objective:To investigate the expression and role of RORα in lipopolysaccharide induced macrophage inflammation in mice.Methods:Mouse bone marrow-derived macrophages(BMDMs)were used and treated with 100 ng/ml lipolypolysaccharide(LPS)at different time points.The transcription levels of inflammatory factors IL-1β and TNF-α,and RORα in the BMDMs were analyzed by quantitative real time PCR(qRT-PCR),and the protein level of RORα was further detected by Western blot.The cells were pretreated with RORα agonist SR1078 and antagonist SR3335 for 24 hours respectively,and then treated with LPS for 2 hours.The concentration of IL-1β,TNF-α and IL-6 in the cell supernatant were measured by ELISA.Results:The transcription levels of IL-1β and TNF-α were significantly upregulated in BMDMs exposed to 100 ng/ml LPS at different time points(P<0.05),while the transcription levels of RORα were significantly inhibited after LPS treatment about 1-12 hours(P<0.05),and the protein expression levels were also significantly decreased(P<0.05).Compared with the LPS treatment group,SR1078 pretreatment significantly inhibited the release of inflammatory cytokines,while SR3335 further activated the release of inflammatory cytokines(P<0.05).Conclusion:RORα can regulate the inflammatory response of mouse BMDMs in response to LPS.

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备注/Memo

备注/Memo:
*空军军医大学第一附属医院学科助推计划项目(XJZT18MJ09)
更新日期/Last Update: 2020-07-28