[1]车 宇,梁 静△,杨怡萍,等.沉默TUG1基因对肝门部胆管癌QBC939细胞增殖、凋亡及周期的影响[J].陕西医学杂志,2020,49(5):538-541，591.
　CHE Yu,LIANG Jing,YANG Yiping,et al.Effects of silencing TUG1 on the proliferation,apoptosis and cell cycle of QBC939 cells[J].,2020,49(5):538-541，591.
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沉默TUG1基因对肝门部胆管癌QBC939细胞增殖、凋亡及周期的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年5期
页码:
538-541，591
栏目:
基础研究
出版日期:
2020-05-05

文章信息/Info

Title:
Effects of silencing TUG1 on the proliferation,apoptosis and cell cycle of QBC939 cells
文章编号:
DOI:10.3969/j.issn.10007377.2020.05.007
作者:
车 宇; 梁 静△; 杨怡萍; 廖 娟; 王 蒨; 蔡英全; 邵 帅
陕西省肿瘤医院放疗科(西安 710061)
Author(s):
CHE Yu; LIANG Jing; YANG Yiping; et al.
Department of Radiotherapy,Shaanxi Cancer Hospital(Xi'an 710061)
关键词:
长链非编码RNA;  牛磺酸上调基因1;  QBC939细胞;  增殖;  凋亡;  细胞周期
Keywords:
Long noncoding RNA;  Taurine-upregulated gene 1;  QBC939 cells;  Proliferation;  Apoptosis;  Cell cycle
分类号:
R735.8
文献标志码:
A
摘要:
目的:研究长链非编码RNA牛磺酸上调基因1(TUG1)对肝门部胆管癌QBC939细胞增殖、凋亡及周期的影响。方法:通过荧光实时定量PCR(RT-QPCR)检测人肝内胆管上皮细胞系HIBEpic、人肝门部胆管癌细胞系QBC939中TUG1的表达; 通过RNA干涉技术沉默QBC939细胞中TUG1的表达,检测siRNA在QBC939细胞中对TUG1-siRNA的沉默效果; MTT法检测沉默TUG1对QBC939细胞增殖的影响; 流式细胞术检测沉默TUG1对QBC939细胞凋亡和周期的影响。结果:肝门部胆管癌细胞QBC939中TUG1的表达较人肝内胆管上皮细胞HIBEpic显著上调; TUG1-siRNA可有效沉默QBC939细胞中TUG1的表达; 沉默TUG1的表达可抑制QBC939细胞的增殖能力,促进QBC939细胞的细胞凋亡,使QBC939细胞周期阻滞于G1期。结论:TUG1在肝门部胆管癌细胞中表达上调并促进肿瘤细胞增殖,是潜在的治疗靶点。
Abstract:
Objective:To investigate the effects of LncRNA TUG1 on the proliferation,apoptosis and cell cycle of human hilar cholangiocarcinoma line QBC939. Methods:TUG1 expression in human intrahepatic cholangiocarcinoma cell line HIBEpic and QBC939 were detected by quantitative real time PCR(RT-QPCR). The expression of TUG1 in QBC939 cells was silenced by RNA interference technology,and the silencing effect of siRNA was detected. MTT method was used to detect the effect of silence TUG1 on the proliferation of QBC939 cells. Flow cytometry was used to detect the effect of TUG1 on the apoptosis and cycle of QBC939 cells. Results:Compared with HIBEpic,the expression level of TUG1 in QBC939 cells was significantly higher,which was effectively silenced by TUG1-siRNA. Silencing the expression of TUG1 could inhibit the proliferation of QBC939 cells,promote the apoptosis of QBC939 cells,and block the cell cycle of QBC939 cells in G1 phase. Conclusion:The expression of TUG1 in hilar cholangiocarcinoma cells is up-regulated and promotes tumor cell proliferation,which is a potential therapeutic target.

参考文献/References:

[1] Marcano-Bonilla L,Mohamed EA,Mounajjed T,et al. Biliary tract cancers:epidemiology,molecular pathogenesis and genetic risk associations [J]. Chin Clin Oncol,2016,5(5):61.
[2] 孙亚利.肝门部胆管癌患者112例根治性切除术后的管理[J].陕西医学杂志,2016,45(10):1438-1439.
[3] 孙瑞华,蔡新生.中医药对大肠癌的精准治疗及研究进展[J].陕西中医,2019,40(11):1653-1656.
[4] Tran TB,Ethun CG,Pawlik TM,et al.Actual 5-year survivors after surgical resection of hilar cholangiocarcinoma[J]. Ann Surg Oncol,2019,26(2):611-618
[5] Haga H,Yan I,Takahashi K,et al. Emerging insights into the role of microRNAs in the pathogenesis of cholangiocarcinoma [J]. Gene Expr,2014,16(2):93-99.
[6] 胡晋平,何丽洁,王汉民.长链非编码RNA在人类疾病中的研究进展[J].陕西医学杂志,2016,45(4):503-504.
[7] Ulitsky I,Bartel DP. lincRNAs:genomics,evolution,and mechanisms[J]. Cell,2013,154(1):26-46.
[8] 谭 楠,刘婷婷,赵 昆.长链非编码RNA H19在非小细胞肺癌患者血清中的表达及临床意义[J].陕西医学杂志,2018,47(5):554-556,570.
[9] Young TL,Matsuda T,Cepko CL. The noncoding RNA taurine up-regulated gene 1 is required for differentiation of the murine retina [J]. Curr Biol,2005,15(6):501-512.
[10] Zhang Q,Geng PL,Yin P,et al. LncRNA TUG1 sponges miR-145 to promote cancer progression and regulate glutamine metabolism via Sirt3/GDH axis[J].Oncotarget,2017,8(69):113650-113661.
[11] Fan S,Yang Z,Ke Z,et al. Downregulation of the long non-coding RNA TUG1 is associated with cell proliferation,migration,and invasion in breast cancer [J]. Biomed Pharmacother,2017,95(2017):1636-1643.
[12] Ji D,Zhong X,Leng K,et al. The role of long non-coding RNA AFAP1-AS1 in human malignant tumors[J]. Pathol Res Pract,2018,214(10):1524-1531.
[13] Zheng WY,Ji DL,Xu Y,et al. NcRNAs and cholangiocarcinoma[J]. J Cancer,2018,9(1):100-107.
[14] Tan Y,Huang Z,Li X,et al. Long non-coding RNA MALAT1 interacts with miR-204 to modulate human hilar cholangiocarcinoma proliferation,migration,and invasion by targeting CXCR4[J]. J Cell Biochem,2017,118(11):3643-3653.
[15] Yu Y,Zhang M,Liu J. Long Non-coding RNA PVT1 promotes cell proliferation and migration by silencing ANGPTL4 expression in cholangiocarcinoma[J].Mol Ther Nucleic Acids,2017,13(2017):503-513.
[16] Li Y,Cai Q,Li W,et al. Long non-coding RNA EPIC1 promotes cholangiocarcinoma cell growth[J]. Biochem Biophys Res Commun,2018,504(4):654-659.
[17] Zhang EB. P53-regulated long non-coding RNA TUG1 affects cell proliferation in human non-small cell lung cancer,partly through epigenetically regulating HOXB7 expression[J]. Cell Death Dis,2014,5(5):e1243.
[18] Huang MD,Chen WM,Qi FZ,et al. Long non-coding RNA TUG1 is up-regulated in hepatocellular carcinoma and promotes cell growth and apoptosis by epigenetically silencing of KLF2[J].Molecular Cancer,2015,14(1):165.

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更新日期/Last Update: 2020-07-28