[1]王 莉.卵巢浆液性癌组织中起始因子EIF5A2和基质金属蛋白酶-21的表达及临床意义*[J].陕西医学杂志,2020,49(4):411-414.
　WANG Li..Expression and significance of EIF5A2 and MMP-21 in ovarian serous carcinoma[J].,2020,49(4):411-414.
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卵巢浆液性癌组织中起始因子EIF5A2和基质金属蛋白酶-21的表达及临床意义*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年4期
页码:
411-414
栏目:
基础研究
出版日期:
2020-04-05

文章信息/Info

Title:
Expression and significance of EIF5A2 and MMP-21 in ovarian serous carcinoma
文章编号:
DOI:10.3969/j.issn.10007377.2020.04.006
作者:
王 莉
锦州医科大学附属第一医院妇科(锦州 121000)
Author(s):
WANG Li.
Department of Gynecology,the First Affiliated Hospital of Jinzhou Medical University(Jinzhou 121000)
关键词:
卵巢浆液性癌;  真核翻译起始因子5A2;  基质金属蛋白酶-21;  增殖;  预后
Keywords:
Ovarian serous carcinoma;  EIF5A2;  MMP-21;  Proliferation;  Prognosis
分类号:
R737.31
文献标志码:
A
摘要:
目的:检测真核翻译起始因子5A2(EIF5A2)和基质金属蛋白酶-21(MMP-21)在卵巢浆液性癌组织中的表达,分析其临床意义,关注两者与增殖指标的关系。方法:选择79例卵巢浆液性癌作为观察组,选择79例卵巢浆液性囊腺瘤作为对照组,留取术后新鲜组织和石蜡包埋组织。应用免疫组化检测两组石蜡包埋组织中EIF5A2、MMP-21的表达,应用Western Blot法检测观察组中增殖细胞核抗原(PCNA)的表达。结果:两组中EIF5A2和MMP-21的表达差别有统计学意义,观察组中EIF5A2和MMP-21的表达在不同脉管累犯、淋巴结转移及肿瘤最大径的分组中差别有统计学意义,在不同年龄、病理分级、神经累犯的分组中无明显差别。EIF5A2和MMP-21、EIF5A2和PCNA、MMP-21和PCNA均具有正相关性。EIF5A2和MMP-21的表达与生存时间有关。结论:EIF5A2和MMP-21高表达且具有正相关性是卵巢浆液性癌重要的分子事件,两者可能对肿瘤的增殖有一定的影响。联合检测两者的表达与患者的预后有关。
Abstract:
Objective:To detect the expression of eukaryotic translation initiation factor 5A2(EIF5A2)and matrix metalloproteinase-21(MMP-21),analyze their clinical significance,and obtain the relationship between them and proliferation indicator. Methods:79 cases of ovarian serous carcinoma were selected as the observation group and 79 cases of ovarian serous cystadenoma as the control group. The fresh tissues and paraffin embedded tissues were collected after operation. The expression of EIF5A2 and MMP-21 in paraffin embedded tissues of the two groups were detected by immunohistochemistry,and PCNA in the observation group was detected by Western blot. Results:The expression of EIF5A2 and MMP-21 in two groups were statistically different. The expression of EIF5A2 and MMP-21 in the observation group were statistically different in vascular involvement,lymph node metastasis and maximum tumor size,but there was no statistically different in age,pathological grade and neural involvement. Positive correlation was found between EIF5A2 and MMP-21,EIF5A2 and PCNA,MMP-21 and PCNA. The expression of EIF5A2 and MMP-21 were correlated with survival time. Conclusion:High expression and positive correlation of EIF5A2 and MMP-21 are important molecular events in ovarian serous carcinoma. EIF5A2 and MMP-21 may have some influence on the proliferation of the tumor. The combined detection of their expression is related to the prognosis of patients.

参考文献/References:

[1] 袁 渊,常晓斌,姚安梅,等. DKK-1在浆液性卵巢癌患者血清的表达及其临床意义[J]. 陕西医学杂志,2015,44(12):1587-1589.
[2] Zhou BF,Wei JH,Chen ZH,et al. Identification and validation of AIB1 and EIF5A2 for noninvasive detection of bladder cancer in urine samples[J]. Oncotarget,2016,7(27):41703-41714.
[3] Zhang J,Pan Q,Yan W,et al. Overexpression of MMP-21 and MMP-28 is associated with gastric cancer progression and poor prognosis[J]. Oncol Lett,2018,15(5):7776-7782.
[4] 袁 渊,姚安梅,周 敏,等. miR-300在浆液性卵巢癌患者血清中的表达及其临床意义[J]. 陕西医学杂志,2015,44(11):26-28.
[5] Skoog T,Elomaa O,Pasonen-Seppanen SM,et al. Matrix metalloproteinase-21 expression is associated with keratinocyte differentiation and upregulated by retinoic acid in Ha CaT cells[J]. J Invest Dermatol,2009,129(1):119-130.
[6] 陈杰伟,陈克明,蔡小嘉,等. 食管癌中真核翻译起始因子5A2的表达及与预后的相关性[J]. 中山大学学报(医学科学版),2017,38(4):512-516.
[7] Sun J,Xu Z,Lv H,et al. eIF5A2 regulates the resistance of gastric cancer cells to cisplatin via induction of EMT[J]. Am J Transl Res,2018,10(12):4269-4279.
[8] Singh H,Samani D,Nambiar N,et al. Effect of matrix metalloproteinase-21(572C/T)polymorphism on HIV-associated neurocognitive disorder[J]. APMIS,2018,126(4):329-336.
[9] Pu Y,Wang L,Wu H,et al. High MMP-21 expression in metastatic lymph nodes predicts unfavorable overall survival for oral squamous cell carcinoma patients with lymphatic metastasis[J]. Oncol Rep,2014,31(6):2644-2650.
[10] Boyd S,Virolainen S,Parssinen J,et al. MMP-21(Stro- melysin-2)and MMP-21 in human and murine squamous cell cancer[J]. Exp Dermatol,2009,18(12):1044-1052.
[11] Wu T,Li Y,Lu J,et al. Increased MMP-21 expression is associated with poor overall survival of patients with gastric cancer[J]. Med Oncol,2013,30(1):323-324.
[12] 张苗苗,王才智.半乳糖凝集素-3和基质金属蛋白酶-9在宫颈癌组织中的表达及其临床意义[J].中华全科医学,2016,14(12):1994-1997,2007.
[13] Khosravi S,Martinka M,Ong CJ.Prognostic significance of the expression of nuclear EIF5A2 in human melanoma[J]. Cancer Res,2015,75(15):4963-4964.
[14] 刘 楠,李 凯,王君兰,等. 痰热清联合舒利迭对慢性阻塞性肺病急性加重患者炎症氧化应激反应的影响[J]. 陕西中医,2017,38(12):1649-1651.
[15] 单宇鹏,张 婕. 益气维血胶囊联合人重组促红素治疗肿瘤相关性贫血疗效及对患者Hepcidin、TFR2水平的影响[J]. 陕西中医,2019,40(12):1717-1719.

备注/Memo

备注/Memo:
*辽宁省自然科学基金资助项目(20180551184)
更新日期/Last Update: 2020-04-13