[1]王玉珏,辛宁宁△.mGluR6对大鼠胚胎神经干细胞生物学功能的影响*[J].陕西医学杂志,2020,49(3):279-284.
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mGluR6对大鼠胚胎神经干细胞生物学功能的影响*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年3期
页码:
279-284
栏目:
基础研究
出版日期:
2020-03-05

文章信息/Info

文章编号:
DOI:〖HT5K〗10.3969/j.issn.10007377.2020.03.005
作者:
王玉珏1辛宁宁2△
1.延安大学医学院医学影像学专业(延安716000); 2.咸阳师范学院校医院(咸阳712000)
关键词:
神经干细胞 代谢型谷氨酸受体6 细胞增殖 细胞凋亡 胚胎 大鼠
分类号:
R392.3
文献标志码:
A
摘要:
目的:探讨代谢型谷氨酸受体6(mGluR6)对大鼠胚胎神经干细胞生物学功能的影响。方法:利用MTT比色法分析mGluR6过表达载体、mGluR6 siRNA在不同时间对大鼠胚胎神经干细胞活力的影响,神经球测量方法分析mGluR6对大鼠神经球增殖的影响,流式细胞术分析mGluR6对大鼠NSCs细胞周期及凋亡的影响。结果:MTT结果表明,在处理24h,48h和72h后,过表达mGluR6显著增强大鼠NSCs的细胞活力,促进NSCs增殖,神经球直径显著增大; mGluR6 siRNA抑制大鼠NSCs的增殖。过表达mGluR6后,与对照组相比G1/G0期和细胞比率显著下降,S期细胞比率显著上升,促进了细胞G1到S期转换; 沉默mGluR6后,G1/G0期和细胞比率显著增加,S期细胞比率显著下降,抑制了大鼠NSCs细胞G1到S 期转换。应用流式细胞术显示,过表达mGluR6 48h后,大鼠NSCs细胞早凋和晚凋均显著下降; 沉默mGluR6显著诱导大鼠NSCs的早凋和晚凋。结论:mGluR6可促进大鼠胚胎神经干细胞的增殖,抑制NSCs凋亡。

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备注/Memo

备注/Memo:
*陕西省科技厅社会发展科技攻关项目(2016SF-190)
更新日期/Last Update: 2020-03-25