[1]龚 磊,吕真冰,喻 晶,等.SIRT1、Phospho-SIRT1在结肠癌组织中的表达及临床意义*[J].陕西医学杂志,2020,49(3):274-278.
　GONG Lei,LV Zhenbing,YU Jing,et al.Expression and clinical significance of SIRT1 and Phospho-SIRT1 in colon cancer tissues[J].,2020,49(3):274-278.
点击复制

SIRT1、Phospho-SIRT1在结肠癌组织中的表达及临床意义*
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年3期
页码:
274-278
栏目:
基础研究
出版日期:
2020-03-05

文章信息/Info

Title:
Expression and clinical significance of SIRT1 and Phospho-SIRT1 in colon cancer tissues
文章编号:
DOI:〖HT5K〗10.3969/j.issn.10007377.2020.03.004
作者:
龚 磊1; 吕真冰1; 喻 晶1; 任明扬1; 田云鸿1; 彭 洪2△
1.川北医学院第二临床学院(四川省南充市中心医院)胃肠外科(南充 637000); 2.川北医学院第二临床学院(四川省南充市中心医院)中西医结合肛肠科(南充 637000)
Author(s):
GONG Lei; LV Zhenbing; YU Jing; et al.
The Second Clinical College of North Sichuan Medical College(Gastrointestinal Surgery,Nanchong Central Hospital,Sichuan Province)(Nanchong 637000)
关键词:
结肠癌;  SIRT1;  Phospho-SIRT1;  TNM分期;  淋巴结转移
Keywords:
Colon cancer;  SIRT1;  Phospho-SIRT1;  TNM stage;  Lymph node metastasis
分类号:
R363.2
文献标志码:
A
摘要:
目的:探讨沉默信息调节因子同源类似物1(SIRT1)和磷酸化沉默信息调节因子同源类似物1(Phospho-SIRT1)在结肠癌组织中的表达及临床意义。方法:选取行手术切除的结肠癌存档蜡块和癌旁正常组织(距病变部位>5cm处,且证实为无癌浸润)石蜡标本各60例。采用Western blot法检测结肠癌组织中SIRT1、Phospho-SIRT1水平,并分析结肠癌患者3年生存率影响因素。结果:SIRT1、Phospho-SIRT1在结肠癌组织中的阳性表达率均高于癌旁正常组织(P<0.05)。TNM分期I～II期的患者SIRT1、Phospho-SIRT1阳性表达率均低于Ⅲ～Ⅳ期患者(P<0.05); P53阳性患者SIRT1阳性表达率低于P53阴性患者,Ki67阳性患者Phospho-SIRT1阳性表达率高于Ki67阴性患者(P<0.05)。单因素分析显示,TNM分期I～II期的患者3年生存率高于Ⅲ～Ⅳ期患者(P<0.05),淋巴结有转移的患者3年生存率低于淋巴结无转移患者(P<0.05); SIRT1、Phospho-SIRT1阳性患者3年生存率均低于阴性患者,差异均有统计学意义(P<0.05)。多因素分析显示,TNM Ⅲ～Ⅳ分期、淋巴结有转移、SIRT1和Phospho-SIRT1阳性表达是影响结肠癌患者预后的独立危险因素(P<0.05)。结论:SIRT1、Phospho-SIRT1在结肠癌组织中的表达均高于癌旁正常组织; SIRT1、Phospho-SIRT1水平与患者的TNM分期和淋巴结转移有关; 其中SIRT1水平与患者P53蛋白表达相关,Phospho-SIRT1水平与患者的Ki67蛋白表达相关; TNM分期、淋巴结转移、SIRT1、Phospho-SIRT1四个因素是影响结肠癌患者预后生存的关键。
Abstract:
Objective:To explore expression and clinical significance of silent information regulator homologues 1(SIRT1)and phosphorylation SIRT1(Phospho-SIRT1)in colon cancer tissues.Methods:Sixty archived paraffin blocks of colon cancer surgically removed in our hospital and sixty paraffin specimens of adjacent normal tissues(more than 5 cm from the lesion and confirmed to be cancer-free infiltration)were enrolled.The levels of SIRT1 and Phospho-SIRT1 in colon cancer tissues were detected by Western blot.The influencing factors of 3-year survival rate of colon cancer patients were analyzed.Results:The positive expression rates of SIRT1 and Phospho-SIRT1 in colon cancer tissues were higher than those in adjacent normal tissues(P<0.05).The positive expression rates of SIRT1 and Phospho-SIRT1 in patients with TNM stage Ⅰ to Ⅱ were lower than those with TNM stage Ⅲ to Ⅳ(P<0.05).The positive expression rate of SIRT1 in patients with positive P53 was lower than that with negative P53.The positive expression rate of Phospho-SIRT1 in patients with positive Ki67 was higher than that with negative Ki-67(P<0.05).Univariate analysis showed that 3-year survival rate of patients with TNM stage Ⅰ to Ⅱ was higher than that of patients with stage Ⅲ to Ⅳ(P<0.05).The 3-year survival rate of patients with lymph node metastasis was lower than that of patients without lymph node metastasis(P<0.05).The 3-year survival rate of patients with positive SIRT1 and Phospho-SIRT1 was lower than that of patients with negative SIRT1 and Phospho-SIRT1(P<0.05).Multivariate analysis showed that TNM stage Ⅲ to Ⅳ,lymph node metastasis,positive expression of SIRT1 and Phospho-SIRT1 were independent risk factors influencing prognosis of patients with colon cancer(P<0.05).Conclusion:The expression of SIRT1 and Phospho-SIRT1 in colon cancer tissues is higher than that in adjacent normal tissues.The levels of SIRT1 and Phospho-SIRT1 are related to TNM staging and lymph node metastasis of patients.The level of SIRT1 is related to the expression of P53 protein.The level of Phospho-SIRT1 is related to the expression of Ki67 protein,TNM staging,lymph node metastasis,SIRT1,and Phospho-SIRT1 are key factors influencing prognosis of patients with colon cancer.

参考文献/References:

[1] 李 黎,朴炳奎,周雍明.通下逐瘀法治疗直肠癌临床研究[J].陕西中医,2019,40(5):561-563.
[2] Deng X,Zheng H,Li D,et al.MicroRNA-34a regulates proliferation and apoptosis of gastric cancer cells by targeting silent information regulator 1[J].Experimental &Therapeutic Medicine,2018,15(4):3705-3714.
[3] 朱文侠,杨 玲,成 钧,等.miR-142-3p在结直肠癌组织与细胞中的表达及对细胞增殖影响研究[J].陕西医学杂志,2018,47(3):282-286.
[4] Edge SB,Compton CC.The American joint committee on cancer:the 7th edition of the AJCC cancer staging manual and the future of TNM[J].Annals of Surgical Oncology,2010,17(6):1471-1474.
[5] Jang SH,Min KW,Paik SS,et al.Loss of SIRT1 histone deacetylase expression associates with tumour progression in colorectal adenocarcinoma[J].J Clin Pathol,2012,65(8):735-739.
[6] 司君利,孟菲菲,崔京远,等.结肠癌中MEG3和GAS5的表达及与预后的关系[J].中国现代普通外科进展,2017,20(9):673-676.
[7] 刘娟娟.复方苦参注射液联合奥沙利铂亚叶酸钙治疗中晚期结直肠癌疗效观察[J].陕西中医,2017,38(10):1409-1410.
[8] 农晰婷,杨 光,张 昱.siRNA靶向抑制MACC_1对结直肠癌细胞增殖和侵袭能力的影响[J].陕西医学杂志,2016,45(1):9-11.
[9] 江继端,李卫东.不同手术类型对老年直肠癌患者术后认知功能的影响[J].西部医学,2018,30(7):80-84,92.
[10] Ma S,Feng J,Zhang R,et al.SIRT1 activation by resveratrol alleviates cardiac dysfunction via mitochondrial regulation in diabetic cardiomyopathy mice[J].Oxidative Medicine and Cellular Longevity,2017,15(2):1-15.
[11] Koronowski KB,Khoury N,Saul I,et al.Neuronal SIRT1(Silent information regulator 2 homologue 1)regulates glycolysis and mediates resveratrol-induced ischemic tolerance.[J].Stroke,2017,48(11):3117-3121.
[12] Lee YH,Song NY,Kim DH,et al.Abstract 1250:Curcumin inhibits migration and growth of human colon cancer cells through covalent modification of oncogenic SIRT1:cysteine 67 as a potential binding site[J].Cancer Research,2017,77(13 Supplement):1250-1250..
[13] 李 合,李 军,陈兴超.miR-34a调节SIRT1表达抑制结肠癌干细胞增殖和侵袭[J].中国组织工程研究,2018,22(17):62-67.
[14] 张新元,隋淑凤,张惠清,等.SIRT1蛋白在胃癌中的表达及其与P-gp、Top-Ⅱα关系的研究[J].中国现代普通外科进展,2018,21(4):257-261,266.
[15] 于 娜,陈建明,李晓才,等.SIRT1对肺癌A549细胞增殖和迁移的影响[J].广东医学,2017,38(1):65-71..
[16] 黄立勇,潘 杰,沈徐宁,等.沉默信息调节因子1在结直肠癌组织中的表达及意义[J].国际肿瘤学杂志,2016,43(3):188-192.
[17] 张亚斌,庞仲辉,姚永锋,等.p53与bcl-2基因变化在股骨头缺血性坏死发病中的影响实验研究[J].陕西医学杂志,2016,45(11):1456-1458.
[18] Gurkan AC,Arisan ED,Yerlikaya PO,et al.Inhibition of autophagy enhances DENSpm-induced apoptosis in human colon cancer cells in a p53 independent manner[J].Cellular Oncology,2018,41(3):297-317.
[19] YangY,Li J,Jin L,et al.Independent correlation between Ki67 index and circulating tumor cells in the diagnosis of colorectal cancer[J].Anticancer Research,2017,37(8):4693-4700.
[20] 贺亚敏,高丽丽,李 惠,等.结肠癌组织中Bmi-1和p53的表达及意义[J].现代肿瘤医学,2017(2):111-114.
[21] 石文静,谭佳妮,沈卫星,等.消癌解毒方对结肠癌的抑制作用及机制探讨[J].中华中医药杂志,2018(1):69-72.
[22] Lee SC,Kim KH,Kim OH,et al.Determination of optimized oxygen partial pressure to maximize the liver regenerative potential of the secretome obtained from adipose-derived stem cells[J].Stem Cell Research &Therapy,2017,8(1):181.
[23] 董玲玲,玉素甫·买买提,郭雅文,等.沉默信息调节因子2同源蛋白1在甲状腺乳头状癌组织中的表达及其临床意义[J].临床外科杂志,2017,25(3):204-207.

相似文献/References:

[1]王 雄,张月飞,赵东雄,等.初诊结肠癌患者血小板相关指标与临床分期关系分析[J].陕西医学杂志,2019,(12):1676.
[2]冯 婷,刘 霞,高立勇.叉头框转录因子O亚族3a对结肠癌SW620细胞的影响及机制研究[J].陕西医学杂志,2021,50(9):1068.[doi:DOI:10.3969/j.issn.1000-7377.2021.09.007]
　FENG Ting,LIU Xia,GAO Liyong.Effect and mechanism of FOXO3a on colon cancer SW620 cells[J].,2021,50(3):1068.[doi:DOI:10.3969/j.issn.1000-7377.2021.09.007]
[3]顾 勇,杨 艳,符 翠,等.白藜芦醇对结肠癌恶性生物学行为的抑制作用研究[J].陕西医学杂志,2021,50(9):1072.[doi:DOI:10.3969/j.issn.1000-7377.2021.09.008]
　GU Yong,YANG Yan,FU Cui,et al.Inhibitory effect of resveratrol on malignant biological behavior of colon cancer[J].,2021,50(3):1072.[doi:DOI:10.3969/j.issn.1000-7377.2021.09.008]
[4]余 亮,郭 建,周国强,等.结肠癌患者血清微小RNA-193a-5p和微小RNA-139-5p表达检测及其对预后的影响[J].陕西医学杂志,2023,52(6):745.[doi:DOI:10.3969/j.issn.1000-7377.2023.06.024]
　YU Liang,GUO Jian,ZHOU Guoqiang,et al.Expression of serum miR-193a-5p and miRNA-139-5p in patients with colon cancer and their effects on prognosis[J].,2023,52(3):745.[doi:DOI:10.3969/j.issn.1000-7377.2023.06.024]
[5]徐 敏,王彦舟,张 华.全身炎性反应指数联合控制营养状况评分对结肠癌患者预后的预测价值[J].陕西医学杂志,2023,52(11):1558.[doi:DOI:10.3969/j.issn.1000-7377.2023.11.023]
　XU Min,WANG Yanzhou,ZHANG Hua.Predictive value of SIRI combined with CONUT score in prognosis of patients with colon cancer[J].,2023,52(3):1558.[doi:DOI:10.3969/j.issn.1000-7377.2023.11.023]
[6]王瑜崴,李 濛,康 博,等.结肠癌组织微小RNA-3607-3p、细胞周期蛋白E2表达及其与患者术后5年预后关系研究[J].陕西医学杂志,2024,(8):1124.[doi:DOI:10.3969/j.issn.1000-7377.2024.08.026]
　WANG Yuwei,LI Meng,KANG Bo,et al.Expression of microRNA-3607-3p and cyclin E2 in colon cancer tissues and their relationship with 5-year prognosis of patients after surgery[J].,2024,(3):1124.[doi:DOI:10.3969/j.issn.1000-7377.2024.08.026]
[7]谭 蕊,李 论,罗 登.飞燕草素调控RhoA/ROCK信号通路对结肠癌细胞生物学行为及血管生成拟态形成的影响[J].陕西医学杂志,2024,(11):1468.[doi:DOI:10.3969/j.issn.1000-7377.2024.11.006]
　TAN Rui,LI Lun,LUO Deng.Effects of delphinidin on the biological behavior and vasculogenic mimicry formation of colon cancer cells by regulating the RhoA/ROCK signaling pathway[J].,2024,(3):1468.[doi:DOI:10.3969/j.issn.1000-7377.2024.11.006]

备注/Memo

备注/Memo:
*四川省卫生健康委员会科研课题(1100478)；川北医学院校级科研发展计划项目( CBY18-A-ZD13)
更新日期/Last Update: 2020-03-25