[1]苏建淳,张国义,卢秋霞,等.miR-124在鼻咽癌组织中的表达及其与预后关系研究*[J].陕西医学杂志,2020,49(2):144-147.
　SU Jianchun,ZHANG Guoyi,LU Qiuxia,et al.Expression of miR-124 in nasopharyngeal carcinoma and its relationship with prognosis[J].,2020,49(2):144-147.
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miR-124在鼻咽癌组织中的表达及其与预后关系研究*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年2期
页码:
144-147
栏目:
基础研究
出版日期:
2020-02-05

文章信息/Info

Title:
Expression of miR-124 in nasopharyngeal carcinoma and its relationship with prognosis
文章编号:
DOI:〖HT5K〗10.3969/j.issn.10007377.2020.02.004
作者:
苏建淳; 张国义; 卢秋霞; 全 强
广东省佛山市第一人民医院鼻咽放疗二科(佛山528000)
Author(s):
SU Jianchun; ZHANG Guoyi; LU Qiuxia; et al.
The Second Department of Nasopharynx Radiotherapy,the First People's Hospital of Foshan City,Guangdong Province(Foshan 528000)
关键词:
鼻咽癌;  微小RNA;  实时荧光定量PCR;  远处转移;  预后;  机制
Keywords:
Nasopharyngeal carcinoma;  MicroRNA;  RT-qPCR;  Distant metastasis;  Prognosis;  Mechanism
分类号:
R739.62
文献标志码:
A
摘要:
目的:探讨miR-124在鼻咽癌组织中的表达及其与预后的关系。方法:收集62例鼻咽癌患者活检标本,以同期62例慢性鼻咽炎患者为对照组。采用实时荧光定量PCR(RT-qPCR)方法检测miR-124在两组组织中的表达情况,分析其表达强度与鼻咽癌转移和预后的关系。结果:鼻咽癌组织中miR-124低表达,与患者年龄、性别无关; 初治时有转移组miR-124表达量显著低于无转移组(P<0.05); TNM分期Ⅲ-Ⅳ期晚期患者miR-124 的表达显著低于Ⅰ-Ⅱ期早期患者(P<0.05); 无瘤生存期(DFS)≥3年组患者miR-124表达量明显高于DFS<3年组(P<0.05)。转染miR-124 siRNA显著提高miR-124在人鼻咽癌CNE-1细胞表达水平,能够显著抑制鼻咽癌细胞的增殖、迁移及侵袭(P<0.05)。 结论:miR-124可能抑制鼻咽癌转移,并对预后有一定影响,其相关分子机制有待深入研究。
Abstract:
Objective:To investigate the expression of miR-124 in nasopharyngeal carcinoma and its relationship with prognosis. Methods:Biopsy specimens of 62 patients with nasopharyngeal carcinoma were collected,and 62 patients with chronic nasopharyngitis were selected as the control group. The expression of miR-124 in the two groups was detected by RT-qPCR. The relationship between the expression intensity and the metastasis and prognosis of nasopharyngeal carcinoma was analyzed. Results:The expression of miR-124 was down-regulated in nasopharyngeal carcinoma,which was not related to the age and sex of the patients. The expression of miR-124 in the metastatic group was significantly lower than that in the non-metastasis group(P<0.05). The expression of miR-124 in patients with advanced TNM stage Ⅲ to Ⅳ was significantly lower than that in patients with stage Ⅰ to Ⅱ(P<0.05). The expression of miR-124 in DFS≥3 years group was significantly higher than that in DFS<3 years group(P<0.05). Transfection of miR-124 siRNA significantly increased the expression of miR-124 in human nasopharyngeal carcinoma CNE-1 cells and inhibited the proliferation,migration and invasion of nasopharyngeal carcinoma cells(P<0.05). Conclusion:MiR-124 may inhibit the metastasis of nasopharyngeal carcinoma and have a certain influence on the prognosis. The related molecular mechanism needs further study.

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备注/Memo

备注/Memo:
*广东省佛山市自筹经费类科技计划项目(2018AB003211)
更新日期/Last Update: 2020-03-25