[1]汪 潇,张 斌△,Hesham Al-Sharani(也门),等.miR-23a、miR-146a在种植体周围炎龈沟液中的表达及临床意义*[J].陕西医学杂志,2020,49(2):135-139.
　WANG Xiao,ZHANG Bin,Hesham Al-Sharani,et al.Expression and clinical significance of miR-23a and miR-146a in gingival crevicular fluid with peri-implantitis[J].,2020,49(2):135-139.
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miR-23a、miR-146a在种植体周围炎龈沟液中的表达及临床意义*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年2期
页码:
135-139
栏目:
基础研究
出版日期:
2020-02-05

文章信息/Info

Title:
Expression and clinical significance of miR-23a and miR-146a in gingival crevicular fluid with peri-implantitis
文章编号:
DOI:〖HT5K〗10.3969/j.issn.10007377.2020.02.002
作者:
汪 潇; 张 斌△; Hesham Al-Sharani(也门); 白 雪
锦州医科大学附属第一医院口腔科(锦州 121000)
Author(s):
WANG Xiao; ZHANG Bin; Hesham Al-Sharani; et al.
Department of Stomatology,First Affiliated Hospital of Jinzhou Medical University(Jinzhou 121000)
关键词:
种植体周围炎;  龈沟液;  微小RNA-23a;  微小RNA-146a;  龈沟炎症;  相关性
Keywords:
Peri-implantitis;  Gingival crevicular fluid;  miRNA-23a;  miRNA-146a;  Gingival crevicular inflammation;  Correlation
分类号:
R781.41
文献标志码:
A
摘要:
目的:探究miR-23a、miR-146a在种植体周围炎患者龈沟液中的表达水平及临床意义。方法:选取56例种植体周围炎患者作为种植体周围炎组,同时选取47例健康体检者作为对照组,检测所有受试者菌斑指数(PLI)、探诊深度(PD)、附着水平(CAL)、龈沟出血指数(SBI)。收集龈沟液并采用Elisa法检测其中白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平,采用实时定量PCR法分析其中miR-23a、miR-146a水平。结果:与对照组相比,种植体周围炎组龈沟液中miR-23a、miR-146a表达,TNF-α、IL-1β、IL-6水平,PD、CAL、PLI、SBI水平均显著升高(P<0.05)。miR-23a、miR-146a与TNF-α、IL-1β、IL-6、PD、CAL、PLI、SBI均呈显著正相关(P<0.05)。Logistic多因素回归分析表明,IL-6、PD、CAL、PLI、miR-23a、miR-146a均为影响种植体周围炎发生的独立危险因素。ROC分析显示,miR-23a诊断种植体周围炎的AUC为0.835(95%CI:0.762～0.914); miR-146a的AUC为0.865(95%CI:0.783～0.956)。结论:miR-23a、miR-146a在种植体周围炎患者龈沟液中表达上调与种植体周围炎炎症水平、疾病程度相关,在种植体周围炎发生中具有一定的预测价值。
Abstract:
Objective:To investigate the expression and clinical significance of miR-23a and miR-146a in gingival crevicular fluid of patients with peri-implantitis.Methods:56 patients with peri-implantitis were selected as peri-implantitis group,and 47 healthy people were selected as control group.Plaque index(PLI),depth of exploration(PD),adhesion level(CAL),gingival sulcus hemorrhage index(SBI)were measured in all subjects.Gingival crevicular fluid were collected,in which the levels of IL-1β,TNF-α and IL-6 were detected by Elisa method,and the levels of miR-23a and miR-146a were analyzed by real-time quantitative PCR.Results:Compared with the control group,the expression levels of miR-23a,miR-146a,TNF-α,IL-1β,IL-6 in gingival crevicular fluid,and PD,CAL,PLI and SBI in peri-implantitis group were significantly increased(P<0.05).miR-23a and miR-146a were positively correlated with TNF-α,IL-1β,IL-6,PD,CAL,PLI and SBI(P<0.05).Logistic multivariate regression analysis showed that IL-6,PD,CAL,PLI,miR-23a and miR-146a were independent risk factors for peri-implantitis.The AUC of miR-23a was 0.835(95% CI,0.762 to 0.914),and that of miR-146a was 0.865(95% CI,0.783 to 0.956).Conclusions:The up-regulation of miR-23a and miR-146a in gingival crevicular fluid of patients with peri-implantitis is related to the inflammation level and disease degree of peri-implantitis,and has certain predictive value in the occurrence of peri-implantitis.

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备注/Memo

备注/Memo:
*辽宁省科学技术计划项目(2018223053)
更新日期/Last Update: 2020-03-25