[1]陈宏月,李兴江△.甲状腺全切除术对甲状腺癌患者血清血管内皮生长因子、可溶性Fas受体、可溶性Fas受体配体mRNA表达的影响研究[J].陕西医学杂志,2020,49(1):96-98,108.
　CHEN Hongyue,LI Xingjiang..Effect of total thyroidectomy on mRNA expression levels of VEGF,sFas and sFasL in serum of patients with thyroid cancer[J].,2020,49(1):96-98,108.
点击复制

甲状腺全切除术对甲状腺癌患者血清血管内皮生长因子、可溶性Fas受体、可溶性Fas受体配体mRNA表达的影响研究
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年1期
页码:
96-98,108
栏目:
临床研究
出版日期:
2020-01-05

文章信息/Info

Title:
Effect of total thyroidectomy on mRNA expression levels of VEGF,sFas and sFasL in serum of patients with thyroid cancer
文章编号:
DOI:10.3969/j.issn.10007377.2020.01.026
作者:
陈宏月1; 李兴江2△
1.锦州医科大学齐齐哈尔医学院附属第一医院研究生培养基地(齐齐哈尔161000)； 2.齐齐哈尔医学院附属第一医院(齐齐哈尔161000)
Author(s):
CHEN Hongyue; LI Xingjiang.
Graduate Education Base， the First Affiliated Hospital of Qiqihar Medical College，Jinzhou Medical University(Qiqihar 161000)
关键词:
甲状腺癌; 甲状腺全切除术; 血管内皮生长因子; 可溶性Fas受体; 可溶性Fas受体配体
Keywords:
Key words Thyroid cancer; Total thyroidectomy; Vascular endothelial growth factor; Soluble fas receptor; Soluble fas receptor ligand
分类号:
R736.1
文献标志码:
A
摘要:
摘 要 目的：研究甲状腺全切除术对甲状腺癌患者血清血管内皮生长因子（VEGF）、可溶性Fas受体（sFas）、可溶性Fas受体配体（sFasL） mRNA表达的影响。方法：纳入拟行甲状腺全切除术治疗的甲状腺癌患者40例作为研究对象。分别于术前1 d以及术后1 d采集所有受试者的静脉血10 ml，采用反转录聚合酶链反应（RTPCR）法检测VEGF、sFas、sFasL mRNA表达水平，以酶联免疫吸附法检测血清白细胞介素6（IL6）、白细胞介素8（IL8）、肿瘤坏死因子α（TNFα）水平，并作相关性分析。结果：手术后患者血液样本中VEGF、sFas、sFasL mRNA表达水平分别为（0.54±0.02）、（0.38±0.02）、（0.37±0.02），显著低于手术前的（0.76±0.01）、（0.60±0.04）、（0.59±0.04）（均P＜0.05）。手术后患者血清白细胞介素6（IL6）、白细胞介素8（IL8）、肿瘤坏死因子α（TNFα）水平分别为（173.29±16.93）pg/ml、（0.57±0.01）ng/L、（1.39±0.03）ng/L，显著低于手术前的（321.28±24.82）pg/ml、（2.71±0.02）ng/L、（3.41±0.32）ng/L（均P＜0.05）。经Pearson相关性分析可得：甲状腺癌患者血液中VEGF、sFas、sFasL mRNA水平与血清IL6、IL8、TNFα水平均呈正相关关系（均P＜0.05）。结论：甲状腺全切除术可显著下调甲状腺癌患者的VEGF、sFas、sFasL mRNA表达水平，同时下调IL6、IL8、TNFα水平。
Abstract:
Abstract Objective:To study the effect of total thyroidectomy on mRNA expression levels of vascular endothelial growth factor (VEGF),soluble Fas receptor (sFas) and soluble Fas receptor ligand (sFasL) in serum of patients with thyroid cancer. Methods:40 patients with thyroid cancer planned to undergo total thyroidectomy were enrolled as study subjects. 10 ml of venous blood was collected from all subjects one day before surgery and one day after surgery,and the mRNA expression levels of VEGF,sFas,and sFasL were measured by RTPCR. The levels of IL6,IL8,and TNFα were detected by enzymelinked immunosorbent assay，and correlation analysis was performed. Results:The mRNA expression levels of VEGF,sFas,and sFasL in the blood samples of patients after surgery were (0.54±0.02),(0.38±0.02),and (0.37±0.02),respectively,which were significantly lower than those before surgery (0.76±0.01),(0.60±0.04),and (0.59±0.04) (all P＜0.05). The levels of serum IL6,IL8,and TNFα were (173.29±16.93) pg/ml,(0.57±0.01) ng/L,and (1.39±0.03) ng/L after surgery,respectively,which were significantly lower than those before surgery (321.28±24.82) pg/ml,(2.71±0.02) ng/L,and (3.41±0.32) ng/L (all P＜0.05). Pearson correlation analysis showed that the mRNA expression levels of VEGF,sFas and sFasL in the blood of patients with thyroid cancer were positively correlated with the levels of serum IL6,IL8 and TNFα (all P＜0.05). Conclusion:Total thyroidectomy can significantly downregulate the mRNA expression levels of VEGF,sFas and sFasL,as well as the levels of IL6,IL8 and TNFα in patients with thyroid cancer.

参考文献/References:

[1] Eccher A,Girolami I,D’Errico A,et al.management of thyroid nodules in deceased donors with comparison between fine needle aspiration and intraoperative frozen section in the setting of transplantation[J].Prog Transplant,2019,29(4):316320.
[2] Gao L,Lin Y,Jiang Y,et al.Ultrasound characteristics of cervical lesions in patients with radioiodine refractory differentiated thyroid cancer: A strobecompliant article[J].Medicine(Baltimore),2019,98(45):1787617877.
[3] 曾 瑶,孔双喜,徐惠丽.重楼皂苷Ⅰ通过Fas/FasL信号通路增强顺铂对非小细胞肺癌A549细胞增殖及侵袭的抑制作用[J].医学分子生物学杂志,2018,15(5):283288.〖JY〗(下转第108页)〖FL)〗
[4] 沈浩元,邓春燕,戴一菲,等.Fas、FasL蛋白与乳腺癌相关性的临床研究[J].中国现代普通外科进展,2017,20(12):948951.
[5] 罗 冬,周定明,江明万,等.双歧杆菌三联活菌片对溃疡性结肠炎患者免疫功能及Fas/FasL表达的影响[J].现代生物医学进展,2017,17(13):25312533.
[6] 王慧礼,汤延华,丁 振,等.Fas/FasL基因多态性与肝细胞癌易感性的关系[J].临床肿瘤学杂志,2017,22(10):913918.
[7] Pandiyan B,Merrill SJ.A model of the cost of delaying treatment of Hashimotos thyroiditis: thyroid cancer initiation and growth[J].Math Biosci Eng,2019,16(6):80698091.
[8] Gholve C,Kumarasamy J,Damle A,et al.Comparison of serum thyroglobulin levels in differentiated thyroid cancerpatients using inhouse developed radioimmunoassay and immunoradiometric procedures[J].Indian J Clin Biochem,2019,34(4):465471.
[9] Jendrzejewski JP,Sworczak K,Comiskey DF,et al.Clinical implications of GWAS variants associated with differentiated thyroid cancer[J].Endokrynol Pol,2019,70(5):423429.
[10] 王玉玉,颜天铭,王志遥,等.小鼠抗人B72单克隆抗体的制备及对8266细胞表面Fas及FasL的诱导作用[J].中华微生物学和免疫学杂志,2019,39(1):5056.
[11] 张 晶,董 燕,胡宝玲,等.3种神经细胞保护措施对缺氧缺血性脑损伤新生大鼠脑组织Fas/FasL表达的影响[J].中国中西医结合急救杂志,2019,26(1):1115.
[12] 黄垂名,张小波,彭小旅.lncRNA MAGI2AS3调控Fas/FasL通路抑制神经母细胞瘤的生长[J].中国免疫学杂志,2019,35(16):19811986.
[13] 谭涵宇,李建超,彭 俊,等.蛴螬不同途径给药对干性年龄相关性黄斑变性模型Caspase3、FasL、TNFα、NFκB表达的影响[J].湖南中医药大学学报,2018,38(5):499503.
[14] 金 平.不同剂量氟比洛芬酯注射液对分化型甲状腺癌术后镇痛效果和炎症因子水平的影响[J].蚌埠医学院学报,2017,42(6):761763.
[15] 李亚南,李利梅,张秀真,等.甲状腺全切除术对大鼠甲状腺癌组织的生长因子、凋亡因子、免疫调节因子的影响[J].中华实验外科杂志,2018,35(12):22352237.

相似文献/References:

[1]谢新静,李忠原.实时剪切波弹性成像联合甲状腺球蛋白抗体检测在甲状腺癌诊断中的应用研究[J].陕西医学杂志,2021,50(2):154.[doi:DOI:10.3969/j.issn.1000-7377.2021.02.007]
　XIE Xinjing,LI Zhongyuan.Application of real-time shear wave elastography combined with Tg-Ab detection in diagnosis of thyroid cancer[J].,2021,50(1):154.[doi:DOI:10.3969/j.issn.1000-7377.2021.02.007]
[2]林松荣,张 磊,罗金现,等.DNA甲基化修饰在甲状腺癌中的研究进展[J].陕西医学杂志,2021,50(2):254.[doi:DOI:10.3969/j.issn.1000-7377.2021.02.033]
　LIN Songrong,ZHANG Lei,LUO Jinxian,et al.Research progress of DNA methylation in thyroid cancer[J].,2021,50(1):254.[doi:DOI:10.3969/j.issn.1000-7377.2021.02.033]
[3]石维超,张少波.甲状腺癌病理微血管密度与颈部淋巴结转移相关性研究[J].陕西医学杂志,2022,51(11):1449.[doi:DOI:10.3969/j.issn.1000-7377.2022.11.031]
　SHI Weichao,ZHANG Shaobo.Correlation between pathological microvessel density and cervical lymph node metastasis in thyroid cancer[J].,2022,51(1):1449.[doi:DOI:10.3969/j.issn.1000-7377.2022.11.031]
[4]王媛媛,朱 静,刘娜娜,等.溶质载体家族1成员5及谷氨酰胺酶2对人甲状腺癌细胞体外恶性行为的影响及机制研究[J].陕西医学杂志,2023,52(3):344.[doi:DOI:10.3969/j.issn.1000-7377.2023.03.023]
　WANG Yuanyuan,ZHU Jing,LIU Nana,et al.Effect of solute carrier family 1 member 5 and glutaminases 2 on malignant behavior of human thyroid cancer cells in vitro and its mechanism[J].,2023,52(1):344.[doi:DOI:10.3969/j.issn.1000-7377.2023.03.023]

更新日期/Last Update: 2020-02-17