[1]武卫党,郭继文△,张丹凤,等.五味子乙素改善糖酵解反应大鼠心肌缺血再灌注损伤实验研究*[J].陕西医学杂志,2020,49(1):12-15.
　WU Weidang,GUO Jiwen,ZHANG Danfeng,et al.Schisandrin B improves myocardial ischemiareperfusion injury by improving glycolysis[J].,2020,49(1):12-15.
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五味子乙素改善糖酵解反应大鼠心肌缺血再灌注损伤实验研究*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年1期
页码:
12-15
栏目:
基础研究
出版日期:
2020-01-05

文章信息/Info

Title:
Schisandrin B improves myocardial ischemiareperfusion injury by improving glycolysis
文章编号:
DOI:10.3969/j.issn.10007377.2020.01.003
作者:
武卫党; 郭继文△; 张丹凤; 耿蓬勃; 路 艳; 石 磊
西电集团医院（西安 710077）
Author(s):
WU Weidang; GUO Jiwen; ZHANG Danfeng; et al.
XD Group Hospital(Xi’an 710077)
关键词:
心肌缺血; 再灌注损伤; 五味子乙素; 血流动力学; 氧化应激; 大鼠
Keywords:
Key words Myocardial; Ischemiareperfusion injury; Schisandrin B; Hemodynamics; Oxidative stress; Rats
分类号:
R332
文献标志码:
A
摘要:
摘 要 目的：探讨五味子乙素在大鼠离体心脏缺血/再灌注损伤模型中的保护作用及对心肌糖酵解反应的调节作用。方法：采用Langendorff离体心脏灌流方法，建立心肌缺血/再灌注体外模型，通过血流变仪考察心肌血流动力学指标；采用酶标仪检测心肌组织中超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSHPx）活性及丙二醛（MDA）含量，检测心肌组织中半胱氨酸天冬氨酸蛋白酶3（Caspase3）活性，ATP含量及乳酸含量。结果：五味子乙素对缺血/再灌注后大鼠离体心脏血流动力学具有显著影响，可显著升高左室发展压（LVDP）、左室内压上升/ 下降速率（±dp/dt）和心率（HR）；同时明显降低心肌组织中氧化应激反应的发生，减少MDA 含量，升高SOD及GSHPx抗氧化酶活性，此外可明显抑制心肌组织中Caspase3，同时升高心肌组织中ATP及乳酸含量，但以上作用与糖酵解抑制联合使用后显著降低。结论：五味子乙素可提高心肌糖酵解水平，抑制心肌缺血再灌注导致的氧化应激反应，减少心肌细胞线粒体损伤的发生，恢复心肌能量供应并改善缺血再灌注心脏血流动力学。
Abstract:
Abstract Objective：To investigate the protective effect of schisandrin B on isolated rat heart model of ischemiareperfusion injury and its regulation on myocardial glycolysis. Methods：An in vitro model of myocardial ischemia/reperfusion was established by Langendorff method. The hemodynamic parameters of myocardium were measured by hemorheology instrument. The activities of superoxide dismutase(SOD),glutathione peroxidase(GSHPx) and the content of malondialdehyde(MDA) in myocardium were measured by enzyme labeling instrument. The activity of cysteine aspartate protease(caspase3),ATP content and lactic acid content in myocardium were measured. Results：Schisandrin B had a significant effect on the hemodynamics of isolated rat heart after ischemia/reperfusion,which could significantly increase left ventricular development pressure(LVDP),left ventricular pressure rise/fall rate(±dp/dt) and heart rate(HR). at the same time,it could significantly reduce the occurrence of oxidative stress response in myocardial tissue,reduce MDA content,increase SOD and GSHPx antioxidant enzyme activity. in addition,it could significantly inhibit caspase3,increase the contents of ATP and lactate in cardiac tissue,but the above effects were significantly reduced in combination with glycolysis inhibition. Conclusion：Schisandrin B can improve the level of myocardial glycolysis,inhibit the oxidative stress response caused by myocardial ischemiareperfusion,reduce the occurrence of myocardial mitochondrial damage,restore the energy supply of myocardium and improve the hemodynamics of myocardial ischemiareperfusion. 

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备注/Memo

备注/Memo:
*国家自然科学基金青年科学基金资助项目（81503327）
更新日期/Last Update: 2020-02-17