[1]柯东平,毛俊倩,郭甲民,等.SCNN1B与胃癌细胞上皮间质转化相关性实验研究*[J].陕西医学杂志,2019,(9):1115-1118.
　KE Dongping,MAO Junqian,GUO Jiamin,et al.Research on the correlation between SCNN1B and〖JZ〗 epithelialmesenchymal transformation in gastric cancer cells[J].,2019,(9):1115-1118.
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SCNN1B与胃癌细胞上皮间质转化相关性实验研究*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2019年9期
页码:
1115-1118
栏目:
基础研究
出版日期:
2019-09-05

文章信息/Info

Title:
Research on the correlation between SCNN1B and〖JZ〗 epithelialmesenchymal transformation in gastric cancer cells
文章编号:
DOI:〖HT5K〗10.3969/j.issn.10007377.2019.09.003
作者:
柯东平; 毛俊倩; 郭甲民; 马龙安△
陕西省肿瘤医院（西安 710061）
Author(s):
KE Dongping; MAO Junqian; GUO Jiamin; et al.
Shaanxi Provincial Cancer Hospital（Xi’an 710061）
关键词:
胃癌; SCNN1B基因; 上皮间质转化; SGC7901细胞; 钙粘附蛋白E
Keywords:
Key words 〖WT5BZ〗〖HT5”SS〗Gastric cancer; SCNN1B gene; Epithelialmesenchymal transformation; SGC7901 cell; Ecadherin
分类号:
R737.31
文献标志码:
A
摘要:
摘 要 〖HT5K〗目的：探讨SCNN1B基因与人胃癌细胞上皮间质转化的相关性。方法：构建SCNN1B过表达载体，稳定转染人胃癌SGC7901细胞。于裸鼠右前肢皮下接种细胞，制备胃癌裸鼠移植瘤模型。3周后处死裸鼠，观察移植瘤生长情况。分别采用Realtime PCR、Western blot、免疫组化检测移植瘤组织中SCNN1B、Ecadherin，Ncadherin及Vimentin表达情况。结果：SCNN1B过表达抑制胃癌移植瘤增长，提高Ecadherin表达水平，降低Ncadherin和Vimentin表达水平。结论：过表达SCNN1B能够抑制胃癌细胞的生长及上皮间质转化。
Abstract:
Abstract 〖WT5BZ〗〖HT5”SS〗Objective：To investigate the correlation between SCNN1B gene and epithelialmesenchymal transformation of human gastric cancer cells. Methods：SCNN1B overexpression vectors were constructed and the vectors were stably transfected into human gastric cancer SGC7901 cells. The nude mice were injected subcutaneously with cells to establish a transplanted tumor model of human gastric carcinoma. After three weeks，nude mice were executed and growth situation of cancer xenografts in the nude mice was observed. The expression of SCNN1B，Ecadherin，Ncadherin and Vimentin in transplanted tumors were detected by Realtime PCR，Western blot and immunohistochemistry. Results：Overexpression of SCNN1B inhibited the growth of gastric cancer xenografts as well increased the expression of Ecadherin and decreased the expression of Ncadherin and Vimentin. Conclusion：SCNN1B overexpression can inhibit the gastric cancer cell growth and epithelial mesenchymal transition.

参考文献/References:

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备注/Memo

备注/Memo:
*陕西省重点科技创新项目（2014KCT24）
更新日期/Last Update: 2019-09-24