[1]刘 颖,李远辙,周声毅,等.脉冲电磁场调控SREBP1c及氧化应激改善db/db小鼠肝脂肪变性研究*[J].陕西医学杂志,2019,(9):1107-1110，1114.
　LIU Ying,LI Yuanzhe,ZHOU Shengyi,et al.Pulsed electromagnetic fields improves hepatic steatosis 〖JZ〗in db/db mice by SREBP1c and oxidative stress[J].,2019,(9):1107-1110，1114.
点击复制

脉冲电磁场调控SREBP1c及氧化应激改善db/db小鼠肝脂肪变性研究*
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2019年9期
页码:
1107-1110，1114
栏目:
基础研究
出版日期:
2019-09-05

文章信息/Info

Title:
Pulsed electromagnetic fields improves hepatic steatosis 〖JZ〗in db/db mice by SREBP1c and oxidative stress
文章编号:
DOI:〖HT5K〗10.3969/j.issn.10007377.2019.09.001
作者:
刘 颖1; 2; 李远辙2; 周声毅2; 阮俊勇2; 罗二平2; 景 达2; 翟明明2△
1.解放军陆军防化学院（北京 100113）；2.空军军医大学军事生物医学工程学系（西安 710032）
Author(s):
LIU Ying; LI Yuanzhe; ZHOU Shengyi; et al.
Institute of NBC Defense（Beijing 100113）
关键词:
脉冲电磁场; 非酒精性脂肪性肝病; 固醇调节元件结合蛋白1; 氧化应激
Keywords:
Pulsed electromagnetic fields; Nonalcoholic fatty liver disease; Sterol regulatory elementbinding proteins 1; Oxidative stress
分类号:
R737.31
文献标志码:
A
摘要:
摘 要 〖HT5K〗目的：探究脉冲电磁场（PEMF）改善db/db小鼠肝脏脂肪变性的潜在机制，以期为PEMF用于改善非酒精性脂肪性肝病（NAFLD）提供实验依据。方法：通过C57BL/KsJ db/db小鼠构建NAFLD模型，随机分为PEMF组和NAFLD组，对照组由8只C57BL/KsJ小鼠组成。PEMF组每天施加外源性PEMF（20 Gs，1 h）刺激，持续12周，其他组不做任何处理。实验结束后检测空腹血糖、血清胰岛素，计算HOMAIR；检测肝脏甘油三酯、丙二醛、还原性谷胱甘肽及氧化型谷胱甘肽、谷胱甘肽过氧化物酶；检测SREBP1c蛋白表达量。结果： PEMF组胰岛素抵抗水平降低约25%；丙二醛及氧化型谷胱甘肽水平显著下降，而还原性谷胱甘肽与谷胱甘肽过氧化酶水平明显上升；SREBP1c的蛋白表达量较NAFLD组减少约35%左右；脂滴数量和大小均显著降低；肝脏TG含量降低至（4.49±0.86） mmol/gprot。结论：PEMF通过调控SREBP1c及氧化应激改善db/db小鼠肝脏脂肪变性。
Abstract:
Abstract 〖WT5BZ〗〖HT5”SS〗〖JP2〗Objective：The potential mechanism for pulsed electromagnetic fields (PEMF) improves the liver steatosis in db/db mice was investigated. Methods：The 8 C57BL/KsJ mice were as control group and 16 C57BL/KsJ db/db mice were randomly divided into PEMF group and NAFLD group. The PEMF group was given exposure of 15.38 Hz frequency and 20 Gs field intensity 1 h/d for 12 weeks. Then the blood glucose and insulin levels were detected to calculate the HOMAIR. The levels of triglyceride (TG)，malonaldehyde (MDA)，glutathione (GSH) and oxidized glutathione(GSSG) and glutathione peroxidase(GSHPx) were determinated. The expression of SREBP1c protein was determinated. Results：Compared with NAFLD group，the HOMAIR of PEMF group decreased 25% (P＜0.05). Besides，liver steatosis was also ameliorated. Further explorations revealed that PEMF could reduce the expression of SREBP1c and increase the expression of GSHPx and consequently suppress oxidative stress. Conclusion：PEMF attenuates oxidative stress and suppresses the expression of SREBP1c to ameliorate liver steatosis in db/db mice. 〖JP〗

参考文献/References:

[1] 〖ZK（#〗延 华，张粉利，高艳琼，等. 非酒精性脂肪肝与同型半胱氨酸水平及胰岛素抵抗的关系探讨[J] . 陕西医学杂志,2017,46(10):14511452.
[2] 杨蕊旭，范建高. 非酒精性脂肪性肝病的流行特征[J] . 传染病信息,2018，(2):101104.
[3] Ottani V，Monti M G，Morocutti M，et al. Influence of pulsed electromagnetic fields on regenerating rat liver after partial hepatectomy[J] . Journal of Anatomy,1984,139 ( Pt 2)(Pt 2):253263.
[4] 〖JP2〗崔 红，赵美香，刘 毅. 脉冲电磁场对db/db鼠胰岛素抵抗的改善作用[J] . 实用医药杂志,2016，(12):11041107.〖JP〗
[5] Kopprasch S，Srirangan D，Bergmann S，et al. Association between systemic oxidative stress and insulin resistance/sensitivity indices  the PREDIAS study[J] . Clinical Endocrinology,2016,84(1):4854.
[6] Satapati S，Kucejova B，Duarte J A G，et al. Mitochondrial metabolism mediates oxidative stress and inflammation in fatty liver[J] . Journal of Clinical Investigation,2015,125(12):44474462.
[7] Keane K N，Cruzat V F，Carlessi R，et al. Molecular events linking oxidative stress and inflammation to insulin resistance and βcell dysfunction[J] . Oxidative Medicine and Cellular Longevity,2015,2015:115.
[8] 俞泽元，赵荣荣，盖大伟，等. SREBP1c、GRP94在非酒精性脂肪肝中的表达及意义[J] . 中国现代医学杂志,2014,24(17):2327.〖ZK)〗〖JY〗〖HT〗(下转第1114页)〖FL)〗
[9] 〖ZK(#〗Xu J L，Li L Y，Wang Y Q，et al. Hepatocytespecific deletion of BAP31 promotes SREBP1c activation，promotes hepatic lipid accumulation，and worsens IR in mice[J] . J Lipid Res,2018,59(1):3547.〖ZK)〗
[10] 〖ZK(#〗牛海艳，李 海，丁玉平. 非酒精性脂肪性肝病的治疗进展[J] . 临床肝胆病杂志,2018，(2):433437.
[11] 李 玲. 2型糖尿病并发非酒精性脂肪肝与胰岛素抵抗关系的研究[J] . 陕西医学杂志,2014,43(5):575576.
[12] Yuan J，Xin F，Jiang W. Underlying signaling pathways and therapeutic applications of pulsed electromagnetic fields in bone repair[J] . Cellular Physiology and Biochemistry,2018,46(4):15811594.
[13] Ma M，Quan Y，Li Y，et al. Bidirectional modulation of insulin action by reactive oxygen species in 3T3L1 adipocytes[J] . Molecular Medicine Reports,2018,18(1):807814.
[14] Sun W. Effects of B vitamins overload on plasma insulin level and hydrogen peroxide generation in rats[J] . The Chinese Journal of Physiology,2017,60(4):207214.
[15] Anderson E J，Lustig M E，Boyle K E，et al. Mitochondrial H2O2 emission and cellular redox state link excess fat intake to insulin resistance in both rodents and humans[J] . Journal of Clinical Investigation,2009,119(3):573581.
[16] Ferre P，Foufelle F. Hepatic steatosis：a role for de novo lipogenesis and the transcription factor SREBP1c[J] . Diabetes，Obesity & Metabolism,2010,12（2）:8392.
[17] Guo R，Liong E C，So K F，et al. Beneficial mechanisms of aerobic exercise on hepatic lipid metabolism in nonalcoholic fatty liver disease[J] . Hepatobiliary & Pancreatic Diseases International,2015,14(2):139144.

相似文献/References:

[1]刘玲娇,余湘尤,虢小丽.西安市职业人群非酒精性脂肪性肝病患病率及其危险因素调查研究[J].陕西医学杂志,2021,50(1):107.[doi:DOI:10.3969/j.issn.1000-7377.2021.01.029]
　LIU Lingjiao,YU Xiangyou,GUO Xiaoli.Prevalence and risk factors of nonalcoholic fatty liver disease among occupational population in Xi'an[J].,2021,50(9):107.[doi:DOI:10.3969/j.issn.1000-7377.2021.01.029]
[2]李 婧,李小凤,闫 妮,等.胰岛素抵抗代谢评分在中老年人群非酒精性脂肪性肝病筛查中的价值研究[J].陕西医学杂志,2023,52(6):688.[doi:DOI:10.3969/j.issn.1000-7377.2023.06.011]
　LI Jing,LI Xiaofeng,YAN Ni,et al.Value of metabolic score for insulin resistance in screening non-alcoholic fatty liver disease in middle-aged and elderly people[J].,2023,52(9):688.[doi:DOI:10.3969/j.issn.1000-7377.2023.06.011]
[3]赵梦溪,罗 斌,吕建瑞,等.隐丹参酮治疗小鼠非酒精性脂肪性肝病效果及机制研究[J].陕西医学杂志,2023,52(9):1135.[doi:DOI:10.3969/j.issn.1000-7377.2023.09.005]
　ZHAO Mengxi,LUO Bin,LYU Jianrui,et al.Effect and mechanism of cryptotanshinone on nonalcoholic fatty liver disease in mice[J].,2023,52(9):1135.[doi:DOI:10.3969/j.issn.1000-7377.2023.09.005]
[4]梁 娜,张泽天,孙晓冉,等.低密度脂蛋白相关受体蛋白6基因多态性与非酒精性脂肪性肝病发病关系研究[J].陕西医学杂志,2023,52(9):1241.[doi:DOI:10.3969/j.issn.1000-7377.2023.09.030]
　LIANG Na,ZHANG Zetian,SUN Xiaoran,et al.Relationship between low-density lipoprotein receptor-related protein 6 gene polymorphism and occurrence of nonalcoholic fatty liver disease[J].,2023,52(9):1241.[doi:DOI:10.3969/j.issn.1000-7377.2023.09.030]

备注/Memo

备注/Memo:
*国家自然科学基金资助面上项目（81471806） 陕西省重点研发计划项目（2018SF176）
更新日期/Last Update: 2019-09-24