[1]张 慧,陈景志,陈建立△,等.DCC和MKK4基因在结直肠癌组织中的表达及临床意义*[J].陕西医学杂志,2019,(8):968-971.
　ZHANG Hui,CHEN Jingzhi,CHEN Jianli,et al.Expression and clinical significance of DDC and MKK4 genes in colorectal cancer tissue[J].,2019,(8):968-971.
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DCC和MKK4基因在结直肠癌组织中的表达及临床意义*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2019年8期
页码:
968-971
栏目:
基础研究
出版日期:
2019-08-05

文章信息/Info

Title:
Expression and clinical significance of DDC and MKK4 genes in colorectal cancer tissue
文章编号:
DOI:10.3969/j.issn.1000-7377.2019.08.002
作者:
张 慧; 陈景志; 陈建立△; 张 琪; 张国志
华北理工大学附属医院普外科（唐山 063000）
Author(s):
ZHANG Hui ; CHEN Jingzhi; CHEN Jianli ; et al.
Affiliated Hospital of North China University of Technology（Tangshan 063000）
关键词:
结直肠癌; 直肠癌缺失基因; 丝裂原活化蛋白激酶激酶4; 肿瘤分化程度; 淋巴转移; 远处转移
Keywords:
Key words Colorectal cancer; Delected in colorectal carcinoma; Mitogen-activated protein kinase kinase 4;  Tumor division; Lymphatic metastasis; Remote transfer
分类号:
R735.3
文献标志码:
A
摘要:
摘 要 目的：探讨结直肠癌缺失基因（DCC）和丝裂原活化蛋白激酶激酶4（MKK4）在人结直肠癌组织中的表达及临床意义。方法：应用逆转录聚合酶链反应（RT-PCR）法、蛋白质印记（Western-blot）法、免疫组织化学法，对60例结直肠癌组织及对应癌旁组织中的DCC、MKK4基因表达水平进行检测，并结合患者临床病理因素分析DCC、MKK4表达与临床病理特征的关系。结果：结直肠癌组织中DCCmRNA、MKK4mRNA基因表达水平均低于癌旁组织（P＜0.05）;结直肠癌组织中DCC、MKK4蛋白表达水平均低于癌旁组织（P＜0.05）；结直肠癌组织中DCC、MKK4蛋白表达的阳性率低于癌旁组织（P＜0.05）；结合患者临床病理参数发现，DCC、MKK4蛋白表达水平均与肿瘤分化程度、淋巴转移、远处转移有显著相关性（P＜0.05）。结论:DCC、MKK4是结直肠癌的抑制基因，在结直肠癌的发生、发展及转移等过程中发挥一定作用。
Abstract:
Abstract Objective: To investigate the expression and clinical significance of delected in colorectal carcinoma (DCC) and Mitogen-activated pro tein kinase kinase 4 (MKK4) in human colorectal cancer. Methods: The expression levels of DCC and MKK4 genes in 60 colorectal cancer tissues and corresponding adjacent tissues were detected by RT-PCR,Western-blot and immunohistochemistry. The relationship between DCC,MKK4 and clinicopathological characteristics was analyzed in combination with clinical and pathological factors. Results: The results of RT-PCR showed that the expression of DCC,MKK4 in colorectal cancer tissues were lower than those in adjacent tissues (P＜0.05). Western-blot showed that the expression levels of DCC,MKK4 in colorectal cancer tissues were significantly lower than those in adjacent tissues (P＜0.05). Immunohistochemistry showed that the expression levels of DCC,MKK4 in colorectal cancer tissues were lower than those in adjacent tissues (P＜0.05). According to the clinical case factors, the expression levels of DCC/MKK4 were significantly correlated with the degree of differentiation, lymphatic metastasis and distant metastasis (P＜0.05). Conclusion: DCC,MKK4 the suppressor genes of colorectal cancer and play a role in the occurrence, development and metastasis of colorectal cancer.

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备注/Memo

备注/Memo:
*中国煤炭工业协会科学技术研究指导性计划项目（MTKJ2017-331）
更新日期/Last Update: 2019-08-13