[1]高 静,边步荣,高彦东,等.茶氨酸处理脑缺血后大鼠海马血红素加氧酶-1表达及细胞凋亡的影响研究[J].陕西医学杂志,2019,(7):823-826，841.
　GAO Jing,BIAN Burong,GAO Yandong,et al.Effects of theanine tostconditioning for cerebral ischemic rats on hippocampal HO-1 expression and apoptosis[J].,2019,(7):823-826，841.
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茶氨酸处理脑缺血后大鼠海马血红素加氧酶-1表达及细胞凋亡的影响研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2019年7期
页码:
823-826，841
栏目:
基础研究
出版日期:
2019-07-05

文章信息/Info

Title:
Effects of theanine tostconditioning for cerebral ischemic rats on hippocampal HO-1 expression and apoptosis
文章编号:
DOI:10.3969/j.issn.1000-7377.2019.07.002
作者:
高 静1; 边步荣1; 高彦东1; 薛荣亮
1.延安大学第二附属医院（陕西省榆林市第一医院）麻醉科（榆林 719000）；2.西安交通大学第二附属医院麻醉科（西安 710004）
Author(s):
GAO Jing; BIAN Burong; GAO Yandong; et al.
Department of Anesthesiology，The Second Affliated Hospital of Yan’an University-the First Hospital of Yulin(Yulin 719000)
关键词:
脑; 缺血再灌注损伤; 血红素加氧酶-1; 细胞凋亡; 茶氨酸; 动物实验
Keywords:
Key words Cerebral; Ischemia reperfusion injury; HO-1; Apoptosis; Theanine; Animal experiment
分类号:
R743.31
文献标志码:
A
摘要:
摘 要 目的：探讨茶氨酸对大鼠的脑保护作用是通过上调血红素加氧酶-1（HO-1）的表达来实现，还是通过自身抗氧化应激和细胞凋亡的能力来实现。方法： 健康清洁雄性SD大鼠270只，体重180～220 g，采用随机数字表法，将大鼠分为三组（n90）：假手术组（S组），脑缺血再灌注组（IR组），茶氨酸组（TH组），TH组大鼠全脑缺血3 min后尾静脉注射25%茶氨酸溶液4 ml/kg（1000 mg/kg），其余两组分别尾静脉注射生理盐水4 ml/kg。采用四血管阻塞法建立大鼠全脑缺血再灌注模型。分别于再灌注后2,6,12,24,48 h处死，HE染色观察海马形态学变化；IHC染色、实时定量PCR检测HO-1海马表达变化；TUNEL染色、流式细胞仪观察海马细胞凋亡变化。结果： 与S组比较，IR组和TH组海马形态学遭到破坏，HO-1表达增加（P＜0.05），细胞凋亡上升（P＜0.01）；与IR组比较，TH组海马形态学得到改善，各个时间点HO-1表达增加得到抑制（P＜0.05），细胞凋亡减低（P＜0.01）。结论： 茶氨酸的脑保护作用不是通过上调HO-1表达来实现的，而是通过自身的抗氧化应激和细胞凋亡能力来实现。
Abstract:
Abstract Objective:A discussion about the protective effect of theanine on rat brain is achieved by up regulate the expression of HO-1，or through the way of anti-oxidative stress and apotosis.Methods:270 healthy，clean，male SD rats，weighed from 180g~220g，were assigned to three groups randomly. sham operation group (S group)，cerebral ischemia reperfusion group (IR group)，theanine treated group (TH group). Rats in TH group with intravenous injection of 25% theanine solution 4ml/kg（1000mg/kg）when the time of cerebral ischemia reperfusion. The other two groups were with intravenous injection of saline 4ml/kgrespectively.Four vessels occlusion method was used to establish the model of global cerebral ischemia reperfusion. Rats were put to death after 2h，6h，12h，24h，48h of reperfusion，and then observed the histological and cytological changes of hippocampal formation by HE staining，the expression of HO-1 by real-time PCR and observed apoptosis in hippocampal by immunohistochemical staining and TUNEL staining respectively. Results:Compared to S group，the morphology of hippocamal in IR and TH group was destructed，the expression of HO-1 was increased (P＜0.05)，cell apoptosis was increased （P＜0.01）;compared to IR group，the morphology of TH group in hippocampal was improved (P＜0.05)，the increase of HO-1 expression was inhibited (P＜0.05)，cell apoptosis was decreased （P＜0.01）. Conclusion:The protective effect of theanin was achieved by its own effect of against oxidative stress and apoptosis，but not through upregulated the expression of HO-1. 

参考文献/References:

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更新日期/Last Update: 2019-07-12