[1]徐 博,苏军涛,钟 烨,等.外源性Apelin13对重症急性胰腺炎大鼠治疗作用及其机制研究*[J].陕西医学杂志,2019,(2):148-152.
　XU Bo,SU Juntao,ZHONG Ye,et al.Effect and mechanism of exogenous apelin13 on severe acute pancreatitis in rats[J].,2019,(2):148-152.
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外源性Apelin13对重症急性胰腺炎大鼠治疗作用及其机制研究*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2019年2期
页码:
148-152
栏目:
基础研究
出版日期:
2020-02-05

文章信息/Info

Title:
Effect and mechanism of exogenous apelin13 on severe acute pancreatitis in rats
文章编号:
DOI: 10.3969/j.issn.10007377.2019.02.003
作者:
徐 博1; 苏军涛1; 钟 烨1; 崔明新1; 王长友1; 陈建立2; 王晓涛1; 田 炜2; 张国志1△
1.华北理工大学附属医院普外科（唐山 063000）;2.华北理工大学医学实验中心（唐山 063000）
Author(s):
XU Bo; SU Juntao; ZHONG Ye;  et al.
Department of General Surgery,  Affiliated Hospital, North China University of Science and Technology（Tangshan 063000 )
关键词:
急性坏死性胰腺炎; 治疗;  一氧化氮; 一氧化氮合酶; 外源性Apelin13; 机制
Keywords:
Key words 〖JP3〗Acute necrotizing pancreatitis; Therapy; Nitric oxide; Nitric oxide synthase; Apelin13; Mechanism
分类号:
R657.5
文献标志码:
A
摘要:
摘 要 目的：探究NO在重症急性胰腺炎(SAP)损伤中的作用机制,并明确外源性Apelin13对SAP的治疗效果。方法：通过开腹胰胆管注射5%牛磺胆酸钠制作SAP大鼠模型。应用随机数表法将60只SPF级SD大鼠随机分三组，每组20只，分别为空白组、SAP组、治疗组。其中空白组分离胃十二指肠，找到胰胆管，不做其他处理；SAP组及治疗组按照SAP动物模型制作标准进行,治疗组在造模同时通过尾静脉注射外源性Apelin13,SAP组给予注射等量生理盐水。分别于造模后3、6、12、24、48 h采集下腔静脉血同时取其胰腺组织。通过ELISA法检测大鼠血清AMS、IL6、TNFα、NO浓度,通过蛋白免疫印记检测胰腺组织iNOS、eNOS表达情况，胰腺组织HE染色明确胰腺组织病理损伤。结果：同一观察点治疗组与空白组相比,AMS、IL6、TNFα、NO均增高（P＜0.05）,iNOS呈高表达、eNOS则呈现低表达（P＜0.05），胰腺组织损伤较明显。同一观察点SAP组与空白组相比，AMS、IL6、TNFα、NO均明显增高（P＜0.05），iNOS呈高表达、eNOS则呈现低表达（P＜0.05），胰腺组织损伤更显著。同一观察点治疗组与SAP组相比，血清淀粉酶、IL6、TNFα、NO均降低（P＜0.05），iNOS呈低表达、eNOS则呈现高表达（P＜0.05），胰腺组织损伤较轻。结论：外源性Apelin13对大鼠重症急性胰腺炎的治疗有较明显效果，其机制可能是通过上调eNOS表达、下调iNOS表达从而调节体内NO含量。
Abstract:
Abstract Objective：To explore the mechanism of action of NO in SAP injury and to determine the therapeutic effect of exogenous Apelin13 on SAP. Methods：SAP rat model was made by injecting 5% sodium taurocholate into open pancreaticobiliary ducts. 60 SPFgrade SD rats were randomly divided into 3 groups using random number table, 20 in each group, namely blank group, SAP group and treatment group. The blank group separated the gastroduodenum and found the pancreaticobiliary tube, and no other treatment was performed. The SAP group and the treatment group were performed according to the SAP animal model. The treatment group was injected with exogenous Apelin13 through the tail vein. The SAP group was given an injection of the same amount of physiological saline. At the same time, 3, 6, 12, 24 and 48 h after modeling, the inferior vena cava blood was collected and the pancreatic tissue was taken. The concentration of AMS, IL6, TN Fα and NO in rats serum were detected by ELISA. The expression of iNOS and eNOS in pancreas was detected by Western blot. Histopathological lesions of pancreas were confirmed by HE staining. Results：Compared with the blank group, AMS, IL6, TNFαand NO in treatment group were all increased in the same observation point(P<0.05), iNOS was highly expressed, and eNOS was lowly expressed (P<0.05). The damage was obvious. At the same observation point, compared with the blank group, the SAP group had significantly higher AMS, IL6, TNFα and NO (P<0.05), high iNOS expression, and low eNOS expression (P<0.05). Damage was more pronounced. The treatment group compared with SAP group, AMS, IL6, TNFα and NO were decreased in the same observation group (P<0.05), iNOS was low, and eNOS was high (P<0.05).The damage is lighter. Conclusion：Exogenous Apelin13 has a significant effect on the treatment of severe acute pancreatitis in rats. The mechanism may beto upregulate the expression of eNOS and down〖LM〗 regulate the expression of iNOS to regulate NO content in vivo.

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备注/Memo

备注/Memo:
*河北省政府资助临床医学优秀人才培养和基础课题研究 项目计划（361036） 
更新日期/Last Update: 2019-03-13