[1]霍彦龙,郝璞珩,田 道 △.丙泊酚抑制缺氧/复氧HUVECs细胞中miR15b表达 及细胞凋亡的实验研究*[J].陕西医学杂志,2019,(2):144-147.
　HUO Yanlong,HAO Puheng,TIAN Dao..Propofol inhibits miR15b expression and apoptosis in hypoxia/reoxygenation HUVECs[J].,2019,(2):144-147.
点击复制

丙泊酚抑制缺氧/复氧HUVECs细胞中miR15b表达 及细胞凋亡的实验研究*
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2019年2期
页码:
144-147
栏目:
基础研究
出版日期:
2020-02-05

文章信息/Info

Title:
Propofol inhibits miR15b expression and apoptosis in hypoxia/reoxygenation HUVECs
文章编号:
DOI: 10.3969/j.issn.10007377.2019.02.002
作者:
霍彦龙1; 2; 郝璞珩3; 田 道1; 4 △
1.延安大学附属医院（延安716000）;2.陕西省吴起县人民医院麻醉科（吴起717600）; 3.西安医学院第一附属医院神经外科（西安710077）;4. 陕西省荣誉军人康复医院麻醉科（渭南714200）
Author(s):
HUO Yanlong;  HAO Puheng; TIAN Dao.
Affiliated Hospital of Yan’an University(Yan’an 716000)
关键词:
丙泊酚; 微RNAs; 抗凋亡蛋白; 细胞凋亡; 缺氧/复氧细胞模型; 血管内皮细胞
Keywords:
Key words Propofol; MicroRNAs; Antiapoptotic protein; Apoptosis; Hypoxia/reoxygenation cell model; Vascular endothelial cells
分类号:
R915
文献标志码:
A
摘要:
摘 要 〖JP2〗目的：研究丙泊酚在缺氧/复氧细胞模型中对微小RNA15b（miR15b）及血管内皮细胞凋亡的影响。方法：建立缺氧/复氧细胞模型［人脐静脉内皮细胞(HUVECs）］，设置实验组、对照组、空白组，其中实验组HUVECs缺氧/复氧培养24 h后，加入丙泊酚（150 μmol/L）干预培养6 h；实时定量PCR（RTqPCR）检测各组HUVECs 中miR15b 的表达情况；RTqPCR、Westernblot检测各组HUVECs 中miR15b靶基因B淋巴细胞瘤2 (bcl2)的表达情况；流式细胞术检测各组细胞凋亡能力。结果：RTqPCR结果显示，实验组miR15b的表达水平低于对照组（P<0.05）；RTqPCR及Westernblot结果显示，实验组bcl2在mRNA和蛋白水平的表达均高于对照组（P<0.05）；实验组HUVECs的细胞凋亡率低于对照组（P<0.05）。结论：丙泊酚能够抑制缺氧/复氧HUVECs细胞中miR15b的表达，并影响其靶基因抗凋亡蛋白bcl2的表达，从而降低细胞凋亡率。
Abstract:
Abstract Objective：To investigate the effect of Propofol on expression of microRNA15b (miR15b) and vascular endothelial cells apoptosis in hypoxia/reoxygenation cell model. Methods: The hypoxia/reoxygenation cell model ［human umbilical vein endothelial cells(HUVECs)］ was established. The experimental group, the control group and the blank group were set up. HUVECs were incubated with hypoxia/reoxygenation for 24h, then treated with 150 μmol/L Propofol for 6h. The expression of miR15b in each group of HUVECs was detected by realtime quantitative PCR (RTqPCR). The expression of B lymphoblastoma2 (bcl2) expression were detected by RTqPCR and Westernblot. Flow cytometry was used to detect apoptosis in each group. Results: RTqPCR results showed that the expression level of miR15b in the experimental group was lower than that in the control group (P<0.05). The results of RTqPCR and Westernblot showed that the expression of bcl2 mRNA and protein in the experimental group were higher than that in the control (P<0.05). The apoptosis rate of HUVECs in experimental group was lower than that in control group (P<0.05). Conclusion: Propofol can inhibit the expression of miR15b in hypoxia/reoxygenation HUVECs and affect the expression of its target gene bcl2（antiapoptotic protein, thus reducing the apoptosis rate.

参考文献/References:

\[1\] 熊 超,刘 力,魏继承.全身麻醉药物对心肌细胞自噬作用的影响\[J\].临床麻醉学杂志,2017,33（11）：11371140.
\[2\] 常建华,张世平,王 臻,等.丙泊酚联合七氟烷对扁桃体切除术患儿麻醉苏醒期血流动力学及躁动情绪影响研究\[J\].陕西医学杂志,2018,47（7）：908911.
\[3\] Ha JH, Noh HS, Shin IW, et al. Mitigation of H2O2induced autophagic cell death by propofol in H9c2 cardiomyocytes\[J\]. Cell Biol Toxicol, 2012, 28 (1)：1929. 
\[4\] 赖永旭,李艳萍.MicroRNA184靶向作用EPB41L5抑制肾细胞癌存活与迁移研究\[J\].陕西医学杂志,2017,46（3）：294297.
\[5\] 周 斌,徐国海,罗振中.miRNA与神经病理性疼痛的关系研究进展\[J\].临床麻醉学杂志,2014,30（9）：931933.
\[6\] 刘军政,张书艳,高建强.骨髓间充质干细胞对软骨诱导分化过程中MicroRNA调控机制\[J\].陕西医学杂志,2017,46（8）：989991.
\[7\] Yin C,Wang X,Kukreja CR, et al. Endogenous microRNAs induced by heatshock reduce myocardial infarction following ischemiareperfusion in mice \[J\]. FEBS Letters, 2008, 582 (30)：41374142. 
\[8\] Chen Z, Hu Z, Liu Z, et al.Differential microRNA profiling in a cellular hypoxia reoxygenation model upon posthypoxic propofol treatment reveals alterations in autophagy signaling network \[J\]. Oxid Med Cell Longev, 2013, 2013 (6):378484. 
\[9\] 黄玉民.MicroRNA15b下调Bcl2促进耐药肺癌细胞凋亡\[J\].临床肺科杂志，2013，18（11）：20702072.〖ZK)〗
\[10\] 卢 悦,王晶晶,梁广彬,等.microRNA20b调节PPARγ抑制缺氧/复氧诱导的内皮细胞凋亡\[J\].中国中西医结合外科杂志,2017,23（3）：283286.
\[11\] 胡 喆,梁就庆,王晶晶,等.丙泊酚通过诱导自噬保护血管紧张素Ⅱ引起细胞损伤\[J\].广东医科大学学报，2017，35（4）：333341.
\[12\] 徐桂萍,后晓超.丙泊酚与七氟醚对结直肠癌根治术患者缺氧诱导因子1水平的影响\[J\].国际麻醉学与复苏杂志，2017，38（11）：967992.
\[13\] 〖JP3〗Noh HS, Shin IW, Ha JH, et al. Propofol protects the autophagic cell death induced by the ischemia_reperfusion injury in rats \[J\]. Mol Cells, 2010,30 (5) :455460. 〖JP〗
\[14\] Delbridge LMD, Mellor KM, Taylor DJ, et al. Myocardial stress and autophagy: mechanisms and potential therapies \[J\]. Nat Rev Cardiol, 2017,14 (7): 412425. 
\[15\] 金 丹,周 烈,刘启祥,等.miR15b在恶性黑色素瘤A375细胞中的表达及其对细胞转移和侵袭的影响\[J\].医学研究杂志,2017,46（7）：156159.
\[16\] 高 伟,张 硕,梁左迪,等.右美托咪定预处理对内毒素血症大鼠海马区Bcl2、Bax表达及认知功能的影响\[J\].国际麻醉学与复苏杂志,2017,38（9）：773776.

相似文献/References:

[1]张智勇,郑伟,常虎林,等.miR-182在胆囊癌中的表达及其对胆囊癌细胞增殖、侵袭能力的影响[J].陕西医学杂志,2017,(06):689.
[2]杨永斌,杨秀梅.芬太尼复合丙泊酚麻醉在小儿支气管异物取出术中的应用效果[J].陕西医学杂志,2019,(7):882.
　YANG Yongbin,YANG Xiumei..Effect of fentanyl combined with propofol anesthesia on removal of foreign bodies in children[J].,2019,(2):882.
[3]叶 姣.丙泊酚与七氟醚剂量配伍对合并轻度认知功能障碍的老年股骨颈骨折〖JZ〗患者围术期氧化应激反应及术后认知功能的影响*[J].陕西医学杂志,2019,(10):1298.
　YE Jiao..Effects of propofol and sevoflurane on perioperative oxidative stress and postoperative cognitive〖JZ〗 function in elderly patients with femoral neck fracture complicated with mild cognitive impairment[J].,2019,(2):1298.
[4]梁伟华,蒋 淼.丙泊酚减低EZH2对miR-34a抑制效应与肠癌细胞增殖侵袭能力变化的研究[J].陕西医学杂志,2022,51(10):1191.[doi:DOI:10.3969/j.issn.1000-7377.2022.10.004]
　LIANG Weihua,JIANG Miao.Relationship between propofol reducing inhibitory effect of EZH2 on miR-34a and proliferation,invasion of colorectal cancer cells[J].,2022,51(2):1191.[doi:DOI:10.3969/j.issn.1000-7377.2022.10.004]
[5]王岩英,周进国,刘晓宁,等.基于环磷酸腺苷/蛋白激酶A/环磷酸腺苷反应元件结合蛋白通路探究丙泊酚对局灶性脑缺血再灌注大鼠神经功能改善机制[J].陕西医学杂志,2024,(4):455.[doi:DOI:10.3969/j.issn.1000-7377.2024.04.005]
　WANG Yanying,ZHOU Jinguo,LIU Xiaoning,et al.Mechanism of propofol improving neurological function in rats with focal cerebral ischemia-reperfusion based on cAMP/PKA/CREB pathway[J].,2024,(2):455.[doi:DOI:10.3969/j.issn.1000-7377.2024.04.005]

备注/Memo

备注/Memo:
*陕西省教育厅科学研究计划项目（16JK1656）
更新日期/Last Update: 2019-03-13