肺泡灌洗液高通量测序检测在肺部疾病诊疗中的作用研究进展*-《陕西医学杂志》

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文章编号:: DOI:10.3969/j.issn.10007377.2019.12.045

作者:: 付慧1; 陈鹏1; 庞健健2△; 1西安医学院基础医学部生化与分子教研室（西安 710021）；2西安市胸科医院肺结核二科（西安 710100）

关键词:: 肺部感染; 肺泡灌洗液; 高通量测序; 非编码RNA; 循环游离DNA; 细胞外囊泡

分类号:: R3923

文献标志码:: A

摘要:: 摘要在呼吸系统疾病中，需要临床生物标志物用于诊断和评估对治疗的反应及判断预后。BALF包含微生物的核酸、游离DNA，RNA、外泌体等，能更全面的反映肺部的整体情况。NGS可以同时测定几百万甚至上亿条DNA或者RNA序列，利用NGS检测气管肺泡灌洗液，在诊断病原微生物方面，具有快速、不用培养的优点，并在肿瘤相关DNA与RNA的检测方面也有着其他基因学手段无法比拟的优势。肺部不断暴露于来自口咽和其他来源的各种微生物群落，回顾和综合通过BALF检测在健康和不同疾病状态的肺部微生物群落的现状。利用NGS检测BALF在肺部感染特殊病原菌的诊断方面的应用也日渐成熟。BALF中的非编码RNA以及cfDNA的检测可以用来协助诊断肺部疾病及判断疾病的发生发展，并且发现使用BALF EV DNA的液体活检是具有组织特异性的并且极其敏感。应用高通量测序检测肺泡灌洗液的特殊指标，所需时间短，可反复取材，是临床用来诊断、评估、预测肺部疾病，包括各类型感染性疾病及肺部肿瘤的一种新方法。

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[1]付慧,陈鹏,庞健健△.肺泡灌洗液高通量测序检测在肺部疾病诊疗中的作用研究进展[J].陕西医学杂志,2019,(12):1742-封3.

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肺泡灌洗液高通量测序检测在肺部疾病诊疗中的作用研究进展

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