[1]韩 乐,陈文娟△,雷光焰,等.microRNA21、microRNA25在肺癌组织中的表达及意义*[J].陕西医学杂志,2019,(12):1596-1600.
　HAN Le,CHEN Wenjuan,LEI Guangyan,et al.Expression and significance of microRNA21 and microRNA25 in lung cancer tissues[J].,2019,(12):1596-1600.
点击复制

microRNA21、microRNA25在肺癌组织中的表达及意义*
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2019年12期
页码:
1596-1600
栏目:
基础研究
出版日期:
2019-12-05

文章信息/Info

Title:
Expression and significance of microRNA21 and microRNA25 in lung cancer tissues
文章编号:
DOI:10.3969/j.issn.10007377.2019.12.005
作者:
韩 乐1; 陈文娟2△; 雷光焰1; 赵 征2
1.陕西省肿瘤医院胸外科 (西安 710061)；2.陕西省肿瘤医院内三科 (西安 710061)
Author(s):
HAN Le; CHEN Wenjuan; LEI Guangyan; et al.
Department of Chest,Shaanxi Cancer Hospital（Xi’an 710061）
关键词:
microRNA21; microRNA25; 肺癌; 基因表达; 癌旁组织
Keywords:
Key words microRNA21; microRNA25; Lung cancer; Gene expression; Significance; Adjacent tissues
分类号:
R392.3
文献标志码:
A
摘要:
摘 要 目的：探讨microRNA21、microRNA25在肺癌组织中的表达及意义。 方法：选取肺癌患者85例，采用实时荧光定量PCR方法检测患者癌组织标本（研究组）及其癌旁组织（对照组）中microRNA21、microRNA25的表达水平，分析microRNA21、microRNA25表达水平及其与临床病理特征的关系，探讨其表达水平与患者预后关系，分析microRNA21、microRNA25在肺癌组织中表达的相关性。 结果：研究组microRNA21、microRNA25 mRNA相对表达水平显著高于对照组，差异有统计学意义（P＜0.05）；microRNA21、microRNA25表达水平与肺癌患者TNM分期有关(P＜0.05)，与Ⅰ、Ⅱ期患者相比，Ⅲ、Ⅳ期患者癌组织中microRNA21、microRNA25表达水平显著上调(P＜0.05)，而microRNA21、microRNA25表达水平与患者性别、年龄、是否吸烟、肿瘤大小、病理类型、淋巴结转移无明显相关性(P＞0.05)；KaplanMeier分析结果显示，肺癌患者癌组织中microRNA21高表达组中位生存期27.96个月（95% CI：26.79~29.14），5年生存率26.47%，microRNA21低表达组中位生存期37.42个月（95% CI：33.34~41.51），5年生存率76.47%，microRNA21高表达组中位生存期显著短于低表达组，差异有统计学意义(Logrank χ2=10.53，P＜0.05)；microRNA25高表达组中位生存期32.85个月（95% CI：31.28~34.42），5年生存率24.59%，microRNA25低表达组中位生存期45.22个月（95% CI：42.20~48.24），5年生存率66.67%，microRNA25高表达组中位生存期显著短于低表达组，差异有统计学意义(Logrank χ2=17.31，P＜0.05)；Spearman相关性分析结果显示，肺癌组织中microRNA21表达与microRNA25表达呈显著正相关(r=0.645，P＜0.05)。 结论：microRNA21、microRNA25在肺癌组织中表达显著上调，其表达水平与患者临床分期有关，且肺癌组织中microRNA21表达与microRNA25表达呈显著正相关。
Abstract:
Abstract Objective：To explore the expression and significance of microRNA21 and microRNA25 in lung cancer tissues.Methods：Eightyfive patients with lung cancer were enrolled in this study.Realtime quantitative PCR was used to detect the expression levels of microRNA21 and microRNA25 in human cancer tissue specimens (study group) and adjacent tissues (control group).The expression levels of microRNA21 and microRNA25 and their relationship with clinicopathological features were analyzed,and the relationship between expression levels and patient prognosis was explored,and the correlation between microRNA21 expression and microRNA25 expression in lung cancer tissues was analyzed.Results：The relative expression levels of microRNA21 and microRNA25 mRNA in study group were significantly higher than those in control group (P＜0.05).The expression levels of microRNA21 and microRNA25 were correlated with TNM staging of lung cancer patients (P＜0.05).Compared with patients with stage I and II,the expression levels of microRNA21 and microRNA25 were significantly upregulated in cancer tissues of stage III and IV patients (P＜0.05),but the expression levels of microRNA21 and microRNA25 were not significantly correlated with gender,age,smoking or not,tumor size,pathological types and lymph node metastasis (P＞0.05).KaplanMeier analysis showed that the median survival time and 5year survival rate of microRNA21 〖LM〗highexpression group among lung cancer patients were 27.96 months (95 % CI：26.79~29.14) and 26.47%,and the median survival time and 5year survival rate of microRNA21 lowexpression group were 37.42 months (95% CI：33.34~41.51) and 76.47%,and the median survival time in microRNA21 highexpression group was significantly shorter than that in low expression group (χ2=10.53,P＜0.05).The median survival time and 5year survival rate in microRNA25 highexpression group were 32.85 months (95% CI：31.28~34.42) and 24.59%,and the median survival time and 5year survival rate in microRNA25 lowexpression group were 45.22 months (95% CI：42.20~48.24) and 66.67%,and the median survival time in microRNA25 highexpression group was significantly shorter than that in lowexpression group (χ2=17.31,P＜0.05).Spearman correlation analysis results showed microRNA21 expression in lung cancer tissues was significantly positively correlated with microRNA25 expression (r=0.645,P＜0.05).Conclusion：The expressions of microRNA21 and microRNA25 are significantly upregulated in lung cancer tissues,and their expression levels are related to clinical staging of patients.The expression of microRNA21 in lung cancer tissues is positively correlated with the expression of microRNA25.

参考文献/References:

[1] Yang S,Bin L,Fei L,et al.Diagnostic microRNA biomarker discovery for nonsmallcell lung cancer adenocarcinoma by integrative bioinformatics analysis[J] .BioMed Research International,2017,2017(1):19.
[2] 全细云,李 明,陈栋良,等.胃癌组织中microRNA214和MGAT5表达及预后的相关性分析[J] .陕西医学杂志,2019,48(1):119122.
[3] Zhao D,Han W,Liu X,et al.MicroRNA128 promotes apoptosis in lung cancer by directly targeting NIMArelated kinase 2[J] .Thoracic Cancer,2017,8(4):304311.
[4] Su Z,Chen D,Li Y,et al.MicroRNA184 functions as tumor suppressor in renal cell carcinoma[J] .Experimental and Therapeutic Medicine,2015,9(3):961966.
[5] Haiql B,Vanas V,Setinek U,et al.Expression of microRNA21 in nonsmall cell lung cancer tissue increases with disease progression and is likely caused by growth conditional changes during malignant transformation[J] .International Journal of Oncology,2014,44(4):13251334.
[6] Meng X,Xiao C,Zhao Y,et al.Metaanalysis of microarrays:diagnostic value of microRNA21 as a biomarker for lung cancer[J] .Int J Biol Markers,2015,30(3):282285.
[7] Li BS,Zuo QF,Zhao YL,et al.MicroRNA25 promotes gastric cancer migration,invasion and proliferation by directly targeting transducer of ERBB2,1 and correlates with poor survival[J] .Oncogene,2015,34(20):25562565.
[8] Zhao Z,Liu J,Wang C,et al.MicroRNA25 regulates small cell lung cancer cell development and cell cycle through cyclin E2[J] .International Journal of Clinical and Experimental Pathology,2014,7(11):77267734.
[9] 张 强,曹 磊,钟 健,等.微小RNA25在肺癌患者中的临床诊断和应用价值[J] .癌症进展,2017,15(11):13021304.
[10] 王 伟,陈传好.MicroRNA在肿瘤治疗中的研究进展[J] .基因组学与应用生物学,2017,36(7):229234.
[11] 王 波.激活AMPK通过下调微小RNA21水平抑制肺癌细胞增殖及侵袭能力[J] .新医学,2018,49(12):6570.
[12] Li Y,Chen Y,Li J,et al.Codelivery of microRNA21 antisense oligonucleotides and gemcitabine using nanomedicine for pancreatic cancer therapy[J] .Cancer Science,2017,108(7):14931503.
[13] Wang D,Fan Z,Liu F,et al.HsamiR21 and HsamiR29 in tissue as potential diagnostic and prognostic biomarkers for gastric cancer[J] .Cellular Physiology and Biochemistry,2015,37(4):14541462.
[14] 肖 焘,揭志刚.microRNA21在胃癌研究中的进展[J] .重庆医学,2018,47(20):9598.
[15] 唐 曦,胡 娅,徐炎华,等.MicroRNA21、microRNA137和microRNA498在肺腺癌患者血清中表达情况及其临床意义[J] .中国现代医学杂志,2018,28(4):3944.
[16] 张轩斌,彭 飞.microRNA21在非小细胞肺癌患者体内外周血和癌组织中的表达水平及其通过调节PTEN蛋白参与抑癌的机制[J] .中国老年学杂志,2017,37(22):55715573.
[17] Hong Z,Chen KH,Dasgupta A,et al.MicroRNA138 and microRNA25 downregulate mitochondrial calciumUniporter,causing the pulmonary arterial hypertension cancer phenotype[J] .American Journal of Respiratory and Critical Care Medicine,2017,195(4):515529.
[18] Chen X,Ba Y,Ma L,et al.Characterization of microRNAs in serum：a novel class of biomarkers for diagnosis of cancer and other diseases[J] .Cell Research,2008,18(10):9971006.
[19] Chen Z,Wu Y,Meng Q,et al.Elevated microRNA25 inhibits cell apoptosis in lung cancer by targeting RGS3[J] .In Vitro Cellular & Developmental BiologyAnimal,2016,52(1):6267.
[20] Guo Q,Zhang H,Zhang L,et al.MicroRNA21 regulates nonsmall cell lung cancer cell proliferation by affecting cell apoptosis via COX19[J] .International Journal of Clinical and Experimental Medicine,2015,8(6):88358841.
[21] Xiang J,Hang JB,Che JM,et al.MiR25 is upregulated in nonsmall cell lung cancer and promotes cell proliferation and motility by targeting FBXW7[J] .International Journal of Cinical and Experimental Pathology,2015,8(8):91479153.

备注/Memo

备注/Memo:
*陕西省卫生健康委员会科研项目（2018D032）
更新日期/Last Update: 2019-12-19